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Tracer coupling among regenerated amacrine cells in the retina of the goldfish

Published online by Cambridge University Press:  02 June 2009

Peter F. Hitchcock
Peter F. Hitchcock
Affiliation:
Departments of Ophthalmology and Anatomy and Cell Biology, The School of Medicine, The University of Michigan, Ann Arbor
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Abstract

This study sought to characterize the tracer coupling of regenerated amacrine cells in the retina of the goldfish and assess the integration of regenerated neurons into existing retinal circuits. Regeneration of new neurons from injury-induced progenitors was stimulated by surgically excising a small rectangular piece of retina. Several months after regeneration was complete, intracellular injections of Neurobiotin, a gap junction-permeant tracer, were made into single regenerated amacrine cells or nonregenerated (extant) amacrine cells lying outside the regenerated patch. Two groups of amacrine cells were injected: those that in normal retina are tracer coupled and a single type (the radiate amacrine cell) that is not. The data show that regenerated amacrine cells are tracer coupled to each other and to their homologous counterparts outside the patch of regenerated retina. Regenerated radiate cells possess morphologically abnormal dendrites, but these processes can extend out of regenerated retina into surrounding normal retina. Similarly, the dendrites of extant radiate cells, severed by the original lesion, can regenerate into the patch of regenerated retina. These results indicate that in the goldfish retina the cell-specific junctional circuitry present in normal retina is re-created in the regenerated retina, and suggest that regenerated neurons are functionally integrated into the existing retina.

Keywords

Gap junctionsIntracellular injectionsBrain injuryNeuronal regenerationAmacrine cellsTracer coupling
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 463 - 472
Copyright
Copyright © Cambridge University Press 1997

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