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Visual thresholds in mice: Comparison of retinal light damage and hypopigmentation

Published online by Cambridge University Press:  02 June 2009

Jennifer M. Hayes  and
Grant W. Balkema
Jennifer M. Hayes
Affiliation:
Biology Department, Boston College, Chestnut Hill
Grant W. Balkema
Affiliation:
Biology Department, Boston College, Chestnut Hill
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Abstract

In previous electrophysiological experiments from hypopigmented animals (mice, rats, rabbits), single-unit recordings from both retinal ganglion axons and cells in the superior colliculus have demonstrated an increase in threshold in the dark-adapted state which is roughly proportional to the animal's ocular melanin concentration. We have examined the thresholds in hypopigmented mice by using a behavioral water maze screening test and found similar threshold elevations to the electrophysiology. In the present study, we investigated the contribution of retinal light damage to the threshold elevation in an albino mouse strain which is relatively resistant to light damage (C57BL/6J c2J/c2J) and mice with profound retinal degeneration (C57BL/6J rd/rd).

Black or albino littermates (C57BL/6J + / c2J or c2J / c2J) were placed in either constant light (350 cd/m2) or dim cycling light (0.001 cd/m2) for 21 days before testing. The normally pigmented animals had thresholds of 1.00 × 10−5 cd/m2 regardless of their light history. The albino mice (c2J/c2J) maintained in constant light had a slight 0.30 log unit elevation compared to their controls that were maintained in dim cycling light 6.3 × 10−4 cd/m2 (similar to previously published reports).

We examined the retinal morphology of representative animals in semi-thin plastic sections. We could not detect any light damage (overall morphology or cell counts in the outer-nuclear layer) in either the normally pigmented animals or the albino mice (c2J/c2J) maintained in dim cycling light. We found extensive light damage in the albino mice (c2J/c2J) maintained in constant light (virtual absence of photoreceptor outersegments) that corresponded to the slight elevation in threshold. We conclude that the elevation in threshold found in albino mice (c2J/c2J) maintained in dim cycling light is not the result of light damage. These results support our previous findings that the sensitivity defect in hypopigmented animals is proportional to the degree of ocular hypopigmentation.

Keywords

HypopigmentationAlbinoAdaptationRetinal degenerationMouse
Type
Research Articles
Information
Visual Neuroscience , Volume 10 , Issue 5 , September 1993 , pp. 931 - 938
Copyright
Copyright © Cambridge University Press 1993

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References

Balkema, G.W. (1988). Elevated dark-adapted thresholds in albino rodents. Investigative Ophthalmology and Visual Science 29, 544–554.Google ScholarPubMed
Balkema, G.W. & Bunt-Milam, A.H. (1982). Cone outer segment shedding in the goldfish retina characterized with the 3H-Fucose technique. Investigative Ophthalmology and Visual Science 23, 319–331.Google ScholarPubMed
Balkema, G.W. & Dräger, U.C. (1991). Impaired visual thresholds in hypopigmented animals. Visual Neuroscience 6, 557–585.CrossRefGoogle ScholarPubMed
Balkema, G.W. & Dräger, U.C. (1990). Origins of uncrossed retinofugal projections in normal and hypopigmented mice. Visual Neuroscience 4, 594–604.CrossRefGoogle ScholarPubMed
Balkema, G.W., Mangini, N.J. & Pinto, L.H. (1983). Discrete visual defects in pearl mutant mice. Science 219, 1085–1087.CrossRefGoogle ScholarPubMed
Balkema, G.W. & Pinto, L.H. (1982). Electrophysiology of retinal ganglion cells in the mouse: A study of a normally pigmented mouse and a congenic hypopigmentation mutant, pearl. Journal of Neurophysiology 48, 968–980.CrossRefGoogle Scholar
Balkema, G.W., Pinto, L.H., Dräger, U.C. & Vanable, J.W. Jr., (1981). Characterization of abnormalities in the visual system of the mutant mouse pearl. Journal of Neuroscience 1, 1320–1329.CrossRefGoogle ScholarPubMed
Cicerone, C.M. & Green, D.G. (1980). Light adaptation within the receptive-field centre of rat retinal ganglion cells. Journal of Physiology 301, 517–534.CrossRefGoogle ScholarPubMed
Creel, D.J., Conlee, J.W. & King, R.A. (1990). Dark adaptation in human albinos. Clinical Vision Science 5, 81–85.Google Scholar
Dräger, U.C. & Olsen, J.F. (1980). Origins of crossed and uncrossed projections in pigmented and albino mice. Journal of Comparative Neurology 191, 383–412.CrossRefGoogle ScholarPubMed
Green, D.G. & Powers, M.K. (1982). Mechanisms of light adaptation in rat retina. Vision Research 22, 209–216.CrossRefGoogle ScholarPubMed
Green, D.G., Herreros-de-Tejada, P. & Glover, M.J. (1992). Night vision in mice. Investigative Ophthalmology and Visual Science 33, 1407.Google Scholar
Green, D.G., Herreros-de-Tejada, P. & Glover, M.J. (1991). Are albino rats night blind? Investigative Ophthalmology and Visual Science 32, 2366–2371.Google ScholarPubMed
Hayes, J.M. & Balkema, G.W. (1993). Elevated dark-adapted thresholds in hypopigmented mice measured with a water maze screening apparatus. Behavior Genetics 23.CrossRefGoogle ScholarPubMed
Herreros-de-Tejada, P., Green, D.G. & Glover, M.J. (1990). Do albino rats have elevated dark-adapted thresholds? Investigative Ophthalmology Visual Science 31, 391.Google Scholar
Herreros-de-Tejada, P., Green, D.G. & Muñoz, C. (1992). Electro-physiological estimates of absolute threshold in rats. Investigative Ophthalmology and Visual Science 33, 1408.Google Scholar
Vail, M.La, Gorrin, G.M., Repaci, M.A., Thomas, L.A. & Ginsberg, H.M. (1987). Genetic regulation of light damage to photoreceptors. Investigative Ophthalmology and Visual Science 28, 1043–1048.Google Scholar
Vail, J.H.La, Nixon, R.A. & Sidman, R.L. (1978). Genetic control of retinal ganglion cell projections. Journal of Comparative Neurology 182, 399–421.Google Scholar
Mangini, N.J., Vanable, J.W., Williams, M.W. & Pinto, L.H. (1985). The optokinetic nystagmus and ocular pigmentation of hypopigmentation mouse mutants. Journal of Comparative Neurology 241, 191–209.CrossRefGoogle ScholarPubMed
Noell, W.K. (1980). There are different kinds of retinal damage in the rat. In The Effects of Constant Light on Visual Processes, ed. Williams, R.P. & Baker, B.N., pp. 357–387. New York: Plenum Press.Google Scholar
Noell, W.K., Walker, V.S., Kang, B.S. & Berman, S. (1966). Retinal damage by light in rats. Investigative Ophthalmology 5, 450–473.Google ScholarPubMed
Powers, M.K. & Green, D.G. (1978). Single retinal ganglion cell responses in the dark-reared rat: Grating acuity, contrast sensitivity, and defocusing. Vision Research 18, 1533–1539.CrossRefGoogle ScholarPubMed
Sanderson, K.J., Guillery, R.W. & Shackelford, R.M. (1974). Congenitally abnormal visual pathways in mink (Mustela vision) with reduced retinal pigment. Journal of Comparative Neurology 154, 225–248.CrossRefGoogle ScholarPubMed
Suzuki, H. & Pinto, L.H. (1986). Response properties of horizontal cells in the isolate retina of wild-type and pearl mutant mice. Journal of Neuroscience 6, 1122–1128.CrossRefGoogle ScholarPubMed