Skip to main content
×
Home
    • Aa
    • Aa

Biochemical and functional characterisation of eggshell matrix proteins in hens

  • Y. Nys (a1), J. Gautron (a1), M. D. McKee (a2), J. M. Garcia-Ruiz (a3) and M. T. Hincke (a4)...
Abstract

The eggshell of the hen is a highly ordered, mineralised structure deposited within an acellular milieu – the uterine fluid secreted by the distal oviduct. Spherulitic crystal growth is initiated by deposition of calcium carbonate on aggregates of organic material present on the outer surface of the eggshell membranes. Gel electrophoresis reveals a complex array of proteins in uterine fluid and eggshell extracts. The eggshell matrix proteins can be classified as egg white proteins (lysozyme, ovalbumin, ovotransferrin, clusterin), bone protein (osteopontin), or proteins specific to the uterus and eggshell (ovocleidins-17 and –116; ovocalyxins-32 and –36). Eggshell extracts, uterine fluid and purified fractions are able to modify the morphology of calcite crystals in vitro. In young hens the breaking strength of the eggshell is inversely related to the degree of calcite orientation. Conversely, reduced strength in eggshell from aged hens coincides with a high variability in crystallographic texture. In guinea fowl the exceptional mechanical properties of the eggshell are explained by an increase in the amount of eggshell produced and particular features of the crystallographic texture. These observations suggest that the eggshell matrix influences the process of crystal growth by controlling size, shape and orientation of calcite crystals. This structural control probably contributes in a substantial manner to the mechanical properties of eggshell.

Copyright
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

L. Addadi and S. Weiner (1992) Control and design principles in biological mineralization. Angewandte Chemie (International Edition in English) 31: 153169

A. Ar , H. Rahn and C.V. Paganelli (1979) The avian egg: mass and strength. Condor 81: 331337

J.L. Arias , D.J. Fink , S.Q. Xiao , A.H. Heuer and A.I. Caplan (1993) Biomineralization and eggshells: cell-mediated acellular compartments of mineralized extracellular matrix. International Review of Cytology 145: 217250

R.G. Board (1982) Properties of avian eggshells and their adaptative value. Biological Review 57: 128

D.A. Carrino , J.P. Rodriguez and A.I. Caplan (1997) Dermatan sulfate proteoglycans from the mineralized matrix of the avian eggshell. Connective Tissue Research 36: 175193

S.D. Chowdhury (1990) Shell membrane system in relation to lathyrogen toxicity and copper deficiency. World's Poultry Science Journal 46: 153169

J.E. Dennis , S.Q. Xiao , M. Agarval , D.J. Fink , A.H. Heuer and A.I. Caplan (1996) Microstructure of matrix and mineral components of eggshells from white leghorn chicken (Gallus gallus). Journal of Morphology 228: 287306

J.M. Dominguez-Vera , J. Gautron , J.M. Garcia-Ruiz and Y. Nys (2000) The effect of avian uterine fluid on the growth behavior of calcite crystals. Poultry Science 79: 901907

M.S. Fernandez , M. Araya and J.L. Arias (1997) Eggshells are shaped by a precise spatio temporal arrangement of sequentially deposited macromolecules. Matrix Biology 16: 1320

M.S. Fernandez , A. Moya , L. Lopez and J.L. Arias (2001) Secretion pattern, ultrastructure and function of extracellular matrix molecules involved in eggshell formation. Matrix Biology 19: 793803

J. Gautron , M. Bain , S. Solomon and Y. Nys (1996) Soluble matrix of hen's eggshell extracts changes in vitro the rate of calcium carbonate precipitation and crystal morphology. British Poultry Science 37: 853866

J. Gautron , M.T. Hincke and Y. Nys (1997) Precursor matrix proteins in the uterine fluid change with stages of eggshell formation in hens. Connective Tissue Research 36: 195210

J. Gautron , M.T. Hincke , M. Panhéleux , J. Garcia-Ruiz , T. Boldicke and Y. Nys (2001a) Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer. Connective Tissue Research 42: 113

J. Gautron , M.T. Hincke , K. Mann , M. Panhéleux , M. Bain , M.D. McKee , S.E. Solomon and Y. Nys (2001b) Ovocalyxin-32, a novel chicken eggshell matrix protein: isolation, amino acid sequencing, cloning and immunocytochemical localization. Journal of Biological Chemistry 276: 3924339252

M.T. Hincke (1995) Ovalbumin is a component of the chicken eggshell matrix. Connective Tissue Research 31: 227233

M.T. Hincke , A.M. Bernard , E.R. Lee , C.P. Tsang and R. Narbaitz (1992) Soluble protein constituents of the domestic fowl's eggshell. British Poultry Science 33:505516

M.T. Hincke , C.P. Tsang , M. Courtney , V. Hill and R. Narbaitz (1995) Purification and immunochemistry of a soluble matrix protein of the chicken eggshell (ovocleidin 17). Calcified Tissue International 56: 578583

M.T. Hincke , J. Gautron , C.P. Tsang , M.D. McKee and Y. Nys (1999) Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116. Journal of Biological Chemistry 274: 3291532923

M.T. Hincke , J. Gautron , M. Panheleux , J.M. Garcia-Ruiz , M.D. McKee and Y. Nys (2000a) Identification and localization of lysozyme as a component of the eggshell membranes and shell matrix. Matrix Biology 19: 443453

G.K. Hunter and H.A. Goldberg (1994) Modulation of crystal formation by bone phosphoproteins: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein. Biochemical Journal 302: 175179

G. Krampitz and G. Graser (1988) Molecular mechanisms of biomineralization in the formation of calcified shells. Angewandte Chemie (International Edition in English) 27: 11451156

L. Lavelin , N. Yarden , S. Ben-Bassat , A. Bar and M. Pines (1998) Regulation of osteopontin gene expression during egg shell formation in the laying hen by mechanical strain. Matrix Biology 17: 615623

K. Mann (1999) Isolation of a glycosylated form of the chicken eggshell protein ovocleidin and determination of the glycosylation site. Alternative glycosylation/phosphorylation at an N-glycosylation sequon. FEBS Letters 463: 1214

M.D. McKee and A. Nanci (1996) Osteopontin: an interfacial extracellular matrix protein in mineralized tissues. Connective Tissue Research 35: 197205

Y. Nys (1987) Progesterone and testosterone elicit increases in the duration of shell formation in domestic hens. British Poultty Science 28: 5768

Y. Nys , J. Zawadzki , J. Gautron and A.D. Mills (1991) Whitening of brown-shelled eggs: mineral composition of uterine fluid and rate of protoporphyrin deposition. Poultry Science 70: 12361245

M. Panheleux , M. Bain , M.S. Fernandez , I. Morales , J. Gautron , J.L. Arias , S.E. Solomon , M. Hincke and Y. Nys (1999a) Organic matrix composition and ultrastructure of eggshell: a comparative study. British Poultry Science 40: 240252

M. Panheleux , O. Kalin , J. Gautron and Y. Nys (1999b) Features of eggshell formation in guinea fowl: kinetics of shell deposition, uterine protein secretion and uterine histology. British Poultry Science 40: 632643

M. Panheleux , Y. Nys , J. Williams , J. Gautron , T. Boldicke and M.T. Hincke (2000) Extraction and quantification by ELISA of eggshell organic matrix proteins (ovocleidin-17, ovalbumin, ovotransferrin) in shell from young and old hens. Poultry Science 79: 580588

M. Pines , V. Knopov and A. Bar (1995) Involvement of osteopontin in egg shell formation in the laying chicken. Matrix Biology 14: 765771

C. Quintana and D. Sandoz (1978) Coquille de l'oeuf de caille: étude ultrastructurale et cristallographique. Calcified Tissue Research 25: 145149

R.M. Sharp and H. Silyn-Roberts (1984) Development of preferred orientation in the eggshell of the domestic fowl. Biophysical Journal 46: 175179

T.M. Wu , J.P. Rodriguez , D.J. Fink , D.A. Carrino , J. Blackwell , A.I. Caplan and A.H. Heuer (1995) Crystallization studies on avian eggshell membranes: implications for the molecular factors controlling eggshell formation. Matrix Biology 14: 507513

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

World's Poultry Science Journal
  • ISSN: 0043-9339
  • EISSN: 1743-4777
  • URL: /core/journals/world-s-poultry-science-journal
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 31 *
Loading metrics...

Abstract views

Total abstract views: 272 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 27th June 2017. This data will be updated every 24 hours.