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Efficacy of dietary vitamin D and its metabolites in poultry - review and implications of the recent studies


Vitamin D plays many important functions in the animal, as it is necessary for proper calcium (Ca) absorption and use in different biological processes. The aim of several recent experiments with poultry was to evaluate the efficacy of different forms and levels of vitamin D3 in the diets for broiler chickens and laying hens. The results of these studies are presented and discussed in this review paper. Based on these results, the modern high-performing poultry requirement for supplemental vitamin D to maximise mineral digestibility, performance and immunity indices, bone health, and eggshell quality is about 3,000 IU/kg, i.e. much higher than NRC (1994) recommendations. The results of several recent poultry studies have shown that 25-hydroxycholecalciferol (25-OH-D3) is more efficient in commercial poultry nutrition than the basic form of vitamin D3 (cholecalciferol).

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A. ATENCIO , H.M. EDWARDS and G.M. PESTI (2005a) Effect of the level of cholecalciferol supplementation of broiler breeder hen diets on the performance and bone abnormalities of the progeny fed diets containing various levels of calcium or 25-cholecalciferol. Poultry Science 84: 1539-1603.

A. ATENCIO , G.M. PESTI and H.M. EDWARDS (2005b) Twenty-five hydroxycholecalciferol as a cholecalciferol substitute in broiler breeder hen diets and its effect on the performance and general health of the progeny. Poultry Science 84: 1277-1285.

A. ATENCIO , H.M. EDWARDS , G.M. PESTI and G.O. WARE (2006) The vitamin D3 requirement of broiler breeders. Poultry Science 85: 674-692.

A. BAR , V. RAZAPHKOVSKY , E. VAX and I. PLAVNIK (2003) Performance and bone development in broiler chickens given 25-hydroxycholecalciferol. British Poultry Science 44: 224-233.

L.C. BROWNING and A.J. COWIESON (2014) Vitamin D fortification of eggs for human health. Journal of the Science of Food and Agriculture 94: 1389-1396.

L.C. BROWNING and A.J. COWIESON (2015) Interactive effects of vitamin D3 and strontium on performance, nutrient retention, and bone mineral composition in laying hens. Journal of the Science of Food and Agriculture 95: 1080-1087.

S. COLET , R.G. GARCIA , I.C.L. ALMEIDA PAZ , F.R. CALDARA , R. BORILLE , A.F.B. ROYER , I.A. NAAS and S. SGAVIOLI (2015) Bone characteristics of broilers supplemented with vitamin D. Revista Brasileira de Ciência Avícola 17: 325-332.

V. DUARTE , C.S. MINAFRA , F.R.D. SANTOS and F.D.S. PERIM (2015) Inclusion of canthaxanthin and 25-hydroxycholecalciferol in the diet of broiler breeders on performance and incubation parameters. Ciência Rural 45: 2050-2055.

H. EBRAHIMI , F. SHARIATMADARI and M.A. KARIMI TORSHIZI (2016) Dietary supplementation and in ovo injection of 1α-OHD3 in a low-calcium and low-phosphorous diets for broilers. Journal of Applied Animal Research 44: 113-117.

H.M. EDWARDS , R.B. SHIRLEY , W.B. ESCOE and G.M. PESTI (2002) Quantitative evaluation of 1-alpha-hydroxycholecalciferol as a cholecalciferol substitute for broilers. Poultry Science 81: 664-669.

C.A. FRITTS and P.W. WALDROUP (2005) Comparison of cholecalciferol and 25-hydroxycholecalciferol in broiler diets designed to minimize phosphorus excretion. The Journal of Applied Poultry Research 14: 156-166.

A.F. GARCIA , A.E. MURAKAMI , C.R.D.A. DUARTE , I. ROJAS , K.P. PICOLI and M. PUZOTTI (2013) Use of Vitamin D3 and its metabolites in broiler chicken feed on performance, bone parameters and meat quality. >Asian-Australasian Journal of Animal Sciences 26: 408-415.

G. GÓMEZ-VERDUZCO , R. MORALES-LÓPEZ and E. AVILA-GOZÀLEZ (2013) Use of 25-hydroxycholecalciferol in diets of broiler chickens: effects on growth performance, immunity and bone calcification. The Journal of Poultry Science 50: 60-64.

J. GREEN and M.E. PERSIA (2012) The effects of feeding high concentrations of cholecalciferol, phytase, or their combination on broiler chicks fed various concentrations of nonphytate phosphorus. The Journal of Applied Poultry Research 21: 579-587.

J.C. HAN , G. CHEN , J. WANG , J. ZHANG , H.X. QU , C. ZHANG , Y. YAN and Y.H. CHENG (2016) Evaluation of relative bioavailability of 25-hydroxycholecalciferol to cholecalciferol for broiler chickens. Asian-Australasian Journal of Animal Sciences 29: 1145-1151.

J.C. HAN , Y.L. WANG , H.X. QU , F. LIANG , J.L. ZHANG , C.X. SHI , X.S. DONG and Y.H. CHENG (2012) One alpha-hydroxycholecalciferol improves growth performance, tibia quality, and meat color of broilers fed calcium-and phosphorus-deficient diets. Asian-Australasian Journal of Animal Sciences 25: 267-271.

J.C. HAN , X.D. YANG , T. ZHANG , H. LI , W.L. LI , Z.Y. ZHANG and J.H. YAO (2009) Effects of 1α-hydroxycholecalciferol on growth performance, parameters of tibia and plasma, meat quality, and type IIb sodium phosphate cotransporter gene expression of one-to twenty-one-day-old broilers. Poultry Science 88: 323-329.

K.C. HUTTON , M.A. VAUGHN , G. LITTA , B.J. TURNER and J.D. STARKEY (2014) Effect of vitamin D status improvement with 25-hydroxycholecalciferol on skeletal muscle growth characteristics and satellite cell activity in broiler chickens. Journal of Animal Science 92: 3291-3292.

S. JIANG , Z. JIANG , K. YANG , F. CHEN , C. ZHENG and L. WANG (2015) Dietary vitamin D3 requirement of Chinese yellow-feathered broilers. Poultry Science 94: 2210-2220.

S. KAPPELI , S.G. GEBHARDT-HENRICH , E. FROHLICH , A. PFULG , H. SCHAUBLIN and M.H. STOFFEL (2011b) Effects of housing, perches, genetics, and 25-hydroxycholecalciferol on keel bone deformities in laying hens. Poultry Science 90: 1637-1644.

K. KESHAVARZ (2003) A comparison between cholecalciferol and 25-OH-cholecalciferol on performance and eggshell quality of hens fed different levels of calcium and phosphorus. Poultry Science 82: 1415-1422.

S.H. KHAN , R. SHAHID , A.A. MIAN , R. SARDAR and M.A. ANJUM (2010) Effect of the level of cholecalciferol supplementation of broiler diets on the performance and tibial dyschondroplasia. Journal of Animal Physiology and Animal Nutrition 94: 584-593.

J. KORELESKI and S. SWIATKIEWICZ (2005a) Efficacy of different levels of a cholecalciferol 25-OH-derivative in diets with two limestone forms in laying hen nutrition. Journal of Animal and Feed Sciences 14: 305-315.

J. KORELESKI and S. SWIATKIEWICZ (2005b) Efficacy of different limestone particle size and 25-hydroxycholecalciferol in broiler diets. Journal of Animal and Feed Sciences 14: 705-714.

M.F. LEDWABA and K.D. ROBERSON (2003) Effectiveness of twenty-five-hydroxycholecalciferol in the prevention of tibial dyschondroplasia in Ross cockerels depends on dietary calcium level. Poultry science 82: 1769-1777.

P. MATTILA , T. ROKKA , K. KONKO , J. VALAJA , L. ROSSOW and E.L. RYHANEN (2003) Effect of cholecalciferol-enriched hen feed on egg quality. Journal of Agricultural and Food Chemistry 51: 283-287.

P. MATTILA , J. VALAJA , L. ROSSOW , E. VENALANEN and T. TUPASELA (2004) Effect of vitamin D2- and D3- enriched diets on egg vitamin D content, production, and bird condition during an entire production period. Poultry Science 83: 433-440.

P.H. MATTILA , E. VALKONEN and J. VALAJA (2011) Effect of different vitamin D supplementations in poultry feed on vitamin D content of eggs and chicken meat. Journal of Agricultural and Food Chemistry 59: 8298-8303.

A. MORRIS , R. SHANMUGASUNDARAM , M.S. LILBURN and R.K. SELVARAJ (2014) 25-Hydroxycholecalciferol supplementation improves growth performance and decreases inflammation during an experimental lipopolysaccharide injection. Poultry Science 93: 1951-1956.

A. MORRIS , R. SHANMUGASUNDARAM , J. MCDONALD and R.K. SELVARAJ (2015) Effect of in vitro and in vivo 25-hydroxyvitamin D treatment on macrophages, T cells, and layer chickens during a coccidia challenge. Journal of Animal Science 93: 2894-2903.

I.D.A. NAAS , M.D.S. BARACHO , L.G.F. BUENO , D.J. DE MOURA , R.D.A. VERCELINO and D.D. SALGADO (2012) Use of vitamin D to reduce lameness in broilers reared in harsh environments. Revista Brasileira de Ciência Avícola 14: 165-172.

G.R. NASCIMENTO DO , A.E. MURAKAMI , A.Q.F.M. GUERRA , I.C. OSPINAS-ROJAS , M.F.Z. FERREIRA and J.C. FANHANI (2014) Effect of different vitamin D sources and calcium levels in the diet of layers in the second laying cycle. Revista Brasileira de Ciência Avícola 16: 37-42.

M.E. PERSIA , M. HIGGINS , T. WANG , D. TRAMPLE and E.A. BOBECK (2013) Effects of long-term supplementation of laying hens with high concentrations of cholecalciferol on performance and egg quality. Poultry Science 92: 2930-2937.

S.V. RAMA RAO , M.V.L.N. RAJU , A.K. PANDA , G. SHYAM SUNDER and R.P. SHARMA (2009) Performance and bone mineralisation in broiler chicks fed on diets with different concentrations of cholecalciferol at a constant ratio of calcium to non-phytate phosphorus. British Poultry Science 50: 528-535.

Z. REN , S. JIANG , Q. ZENG , X. DING , S. BAI , J. WANG , Y LUO , Z. SU , Y. XUAN , B. YAO , F. CISNEROS and K. ZHANG (2016) Effect of dietary canthaxanthin and 25-hydroxycholecalciferol supplementation on the performance of duck breeders under two different vitamin regimens. Journal of Animal Science and Biotechnology 7: 2.

N. SAHIN , T.A. BALCI , O. KUCUK , M.O. SMITH and K. SAHIN (2009) Effects of 25-hydroxycholecalciferol and soy isoflavones supplementation on bone mineralisation of quail. British Poultry Science 50: 709-715.

D. SALVADOR , D.E.D. FARIA , M.R. MAZALLI , D.T. ITO , D.E.D. FARIA FILHO and L.F. ARAÚJO (2009) Vitamins D and C for laying hens at the initial phase of egg production. Revista Brasileira de Zootecnia 38: 887-892.

J.L. SAUNDERS-BLADES and D.R. KORVER (2014) The effect of maternal vitamin D source on broiler hatching egg quality, hatchability, and progeny bone mineral density and performance. The Journal of Applied Poultry Research 23: 773-783.

J.L. SAUNDERS-BLADES and D.R. KORVER (2015) Effect of hen age and maternal vitamin D source on performance, hatchability, bone mineral density, and progeny in vitro early innate immune function. Poultry Science 94: 1233-1246.

M. SANTIAGO , S. DAVID , N. ALEXANDRA , A. EDUARDO and Q.G. JIMMY (2016) Effect of 25-hydroxycholecalciferol (25-OH-D3) on productive performance and bone mineralization in broiler. Open Journal of Animal Sciences 6: 180-184.

Z.W. SUN , L. YAN , J.P. ZHAO , H. LIN and Y.M. GUO (2013) Increasing dietary vitamin D3 improves the walking ability and welfare status of broiler chickens reared at high stocking densities. Poultry Science 92: 3071-3079.

M.R. TATARA , W. KRUPSKI , M. JANKOWSKI , Z. ZDUŃCZYK , J. JANKOWSKI and T. STUDZIŃSKI (2011) Effects of dietary calcium content and vitamin D source on skeletal properties in growing turkeys. British Poultry Science 52: 718-729.

C.A. TORRES , S.L. VIEIRA , R.N. REIS , A.K. FERREIRA , P.X.D. SILVA and F.V.F. FURTADO (2009) Productive performance of broiler breeder hens fed 25-hydroxycholecalciferol. Revista Brasileira de Zootecnia 38: 1286-1290.

K. VIGNALE , E.S. GREENE , J.V. CALDAS , J.A. ENGLAND , N. BOONSINCHAI , P. SODSEE , E.D. POLOCK , S. DRIDI and C.N. COON (2015) 25-hydroxycholecalciferol enhances male broiler breast meat yield through the mTOR pathway. The Journal of Nutrition 145: 855-863.

C.C. WHITEHEAD , H.A. MCCORMACK , L. MCTEIR and R.H. FLEMING (2004) High vitamin D3 requirements in broilers for bone quality and prevention of tibial dyschondroplasia and interactions with dietary calcium, available phosphorus and vitamin A. British Poultry Science 45: 425-436.

R.F. WIDEMAN , J. BLANKENSHIP , I.Y. PEVZNER and B.J. TURNER (2015) Efficacy of 25-OH vitamin D3 prophylactic administration for reducing lameness in broilers grown on wire flooring. Poultry Science 94: 1821-1827.

L. YAO , T. WANG , M. PERSIA , R. L. HORST and M. HIGGINS (2013) Effects of vitamin D3-enriched diet on egg yolk vitamin D3 content and yolk quality. Journal of Food Science 78: C178-C183.

J.G. YARGER , C.A. SAUNDERS , J.L. MCNAUGHTON , C.L. QUARLES , B.W. HOLLIS and R. GRAY (1995) Comparison of dietary 25-hydroxycholecalciferol and cholecalciferol in broiler chickens. Poultry Science 74: 1159-1167.

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World's Poultry Science Journal
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