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Attempts at in vitro fertilization and culture of in vitro matured oocytes in sei (Balaenoptera borealis) and Bryde's (B. edeni) whales

Published online by Cambridge University Press:  01 February 2009

M. M. U. Bhuiyan ,
Y. Suzuki ,
H. Watanabe ,
K. Matsuoka ,
Y. Fujise ,
H. Ishikawa ,
S. Ohsumi  and
Y. Fukui
M. M. U. Bhuiyan
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan.
Y. Suzuki
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan.
H. Watanabe
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan.
K. Matsuoka
Affiliation:
Institute of Cetacean Research, Tokyo 104–0055, Japan.
Y. Fujise
Affiliation:
Institute of Cetacean Research, Tokyo 104–0055, Japan.
H. Ishikawa
Affiliation:
Institute of Cetacean Research, Tokyo 104–0055, Japan.
S. Ohsumi
Affiliation:
Institute of Cetacean Research, Tokyo 104–0055, Japan.
Y. Fukui*
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan. Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan.
*
All correspondence to: Yutaka Fukui. Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555 Hokkaido, Japan. Tel: +81 155 49 5415. Fax: +81 155 49 5593. e-mail: fukui@obihiro.ac.jp
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Summary

The cumulus–oocyte–complexes (COCs) recovery rates with respect to reproductive status per sei (Balaenoptera borealis) and Bryde's (B. edeni) whales were determined in Experiment 1. The number of COCs recovered ranged from 16.0 to 30.6 and from 6.7 to 26.8 per sei and Bryde's whales, respectively. The effects of COCs grades and protein supplementation in embryo culture medium on development of in vitro fertilized (IVF) embryos were evaluated in sei and Bryde's whales in Experiment 2. The COCs were classified into either Grade A (COCs with five or more layers of compact cumulus cells) or Grade B (COCs with less than five layers of compact or expanded cumulus cells) before being cultured for IVM. The cleavage (12.0 to 19.5%), 4-cell (8.0 to 12.0%) and 8-cell (4.0 to 8.0%) formation rates in sei whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either fetal whale serum (FWS)- or bovine serum albumin (BSA)-supplemented medium. The cleavage (4.0 to 14.8%), 4-cell (0.0 to 7.5%) and 8-cell (0.0 to 2.6%) formation rates in Bryde's whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either FWS- or BSA-supplemented medium. The grade B oocytes cultured in FWS-supplemented medium developed to morula stage (1.1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales.

Keywords

In vitro embryo productionOocyte gradeProtein supplementationWhale
Type
Research Article
Information
Zygote , Volume 17 , Issue 1 , February 2009 , pp. 19 - 28
Copyright
Copyright © Cambridge University Press 2008

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