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Synergistic effect of basic fibroblast growth factor (bFGF) and epidermal growth factor on derivation of camel (Camelus dromedarius) trophoblast stem cells

Published online by Cambridge University Press:  20 June 2019

Faisal A. Alzahrani Open the ORCID record for Faisal A. Alzahrani [Opens in a new window]
Faisal A. Alzahrani*
Affiliation:
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia; and Department of Biological Sciences, Rabigh College of Science and Arts, King Abdulaziz University, Rabigh Branch, Rabigh 21911, Kingdom of Saudi Arabia
*
*Address for correspondence: Faisal A. Alzahrani. Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia; and Department of Biological Sciences, Rabigh College of Science and Arts, King Abdulaziz University, Rabigh Branch, Rabigh 21911, Kingdom of Saudi Arabia. E-mail: faahalzahrani@kau.edu.sa
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Summary

This study aimed to optimize the derivation of trophectoderm from in vitro-produced camel embryos under feeder-free culture conditions using the basement membrane matrix Matrigel. Trophoblastic vesicles were obtained through mechanical microdissection of in vitro-produced camel (Camelus dromedarius) embryos. Supplementing the culture medium with 10 ng/ml of epidermal growth factor and 10 ng/ml fibroblast growth factor improved the attachment and subsequent outgrowths of cultured trophoblastic vesicles when compared with the control group and the groups supplemented individually with each growth factor. The expression levels of pluripotency genes octamer-binding transcription factor 4 (Oct4), sex determining region Y-box 2 (Sox2), myelocytomatosis proto-oncogene (c-Myc) and anti-apoptotic gene B-cell lymphoma 2 (Bcl2) were increased in trophoblastic vesicles supplemented with both growth factors when compared with the control group. Conversely, both growth factors decreased the expression of apoptotic genes tumour protein p53 (p53) and Bcl-2-associated X protein (Bax). To the best of our knowledge, this may be the first report describing the derivation of trophoblast stem cells from in vitro-produced camel embryos.

Keywords

bEGFCamelEmbryoEmbryonic stem cellsEFGTrophoblast
Type
Short Communication
Information
Zygote , Volume 27 , Issue 4 , August 2019 , pp. 255 - 258
Copyright
© Cambridge University Press 2019 

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