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Developmental competence of ovine oocyte following vitrification: effect of oocyte developmental stage, cumulus cells, cytoskeleton stabiliser, FBS concentration, and equilibration time

Published online by Cambridge University Press:  14 August 2012

Abolfazl Shirazi ,
Fatemeh Taheri ,
Hassan Nazari ,
Maryam Norbakhsh-nia ,
Ebrahim Ahmadi  and
Banafsheh Heidari
Abolfazl Shirazi
Affiliation:
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Shahid Beheshti University, P.O. Box: 19615-1177, Tehran, Iran. Research Institute of Animal Embryo Technology, Shahrekord University, P. O. Box: 115, Shahrekord, Iran.
Fatemeh Taheri
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P. O. Box: 115, Shahrekord, Iran.
Hassan Nazari
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P. O. Box: 115, Shahrekord, Iran.
Maryam Norbakhsh-nia
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P. O. Box: 115, Shahrekord, Iran.
Ebrahim Ahmadi
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, P. O. Box: 115, Shahrekord, Iran.
Banafsheh Heidari*
Affiliation:
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Shahid Beheshti University, P.O. Box: 19615-1177, Tehran, Iran.
*
All correspondence to: Banafsheh Heidari. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Shahid Beheshti University, P.O. Box: 19615-1177, Tehran, Iran. Tel: +98 21 22404144. Fax: +98 21 22432021. e-mail: ban_heidari@yahoo.com or b.heidari@avicenna.ac.ir
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Summary

The aim of the present study was to examine the effects of fetal bovine serum (FBS) concentration, equilibration time, and oocyte pre-treatment with cytochalasin B (CCB) on subsequent development of vitrified-warmed ovine immature (GVCOCs) and matured (MII) oocytes with (MIICOCs) or without cumulus cells (MIIDOs). In Experiment 1, the effects of FBS concentrations (10 and 20%) during the vitrification-warming procedure were examined. Survival rates after warming were not different between GVCOCs, MIICOCs and MIIDOs oocytes. After in vitro fertilization, rate of cleaved embryos in MIICOCs group at the presence of 20%FBS was higher than MIIDOs and GVCOCs groups. In Experiment 2, the effects of equilibration times (5, 7, and 10 min) were examined. There was no difference in survival rate of vitrified-warmed oocytes equilibrated at different times. Although, the rate of cleavage in MIICOCs and MIIDOs oocytes equilibrated for 10 and 7 min, respectively, was higher than 5 min equilibrated MIIDOs and 7 and 10 min equilibrated GVCOCs oocytes. In Experiment 3, the effects of oocyte pre-treatment with CCB were examined. Despite the insignificant difference in survival rate of vitrified-warmed ovine immature and matured oocytes, the rates of cleavage in CCB pretreated groups were significantly lower than untreated groups. Moreover, the blastocysts were only derived from those cumulus enclosed vitrified-warmed germinal vesicle (GV) and MII oocytes that had been exposed to 10% FBS in the absence of CCB. In conclusion, the presence of cumulus cells, 10% FBS, and the omission of CCB were beneficial for post-warming development of vitrified ovine oocytes.

Keywords

Cumulus cellsCytoskeleton stabilizerOvine oocyteVitrification

Information

Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 165 - 173
Copyright
Copyright © Cambridge University Press 2012 

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