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Double activation improves rabbit freeze–thawed oocytes developmental potential

Published online by Cambridge University Press:  14 August 2009

J. Wang ,
L. Cong ,
Z.G. Zhang ,
Y.X. Cao ,
Z.L. Wei ,
P. Zhou ,
J.H. Zhao  and
X.J. He
J. Wang
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
L. Cong*
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Z.G. Zhang
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Y.X. Cao
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Z.L. Wei
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
P. Zhou
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
J.H. Zhao
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
X.J. He
Affiliation:
Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
*
All correspondence to: L. Cong. Reproduction Centre, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. e-mail: conglin1957@163.com
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Summary

Objective: To investigate the effects of various activation methods on freeze–thawed rabbit oocytes developmental potential. Methods: Rabbit oocytes were vitrified by cryoleafs and cryoprotected with ethylene glycol and propanediol. After thawing, the oocytes were fertilized by intracytoplasmic sperm injection (ICSI). Surviving oocytes after ICSI were divided into five groups at random. Group 1: Oocytes (n = 30) activated 1 h after ICSI by calcium ionomycin (I0634); Group 2: Oocytes (n = 26) activated by strontium chloride an hour after ICSI; Group 3: Oocytes (n = 33) activated by I0634 twice; Group 4: Oocytes (n = 28) were activated by strontium chloride twice; Control Group: Inactivated oocytes (n = 39). Blastocysts derived from each group were transplanted to recipient rabbits. Results: Rates of fertilization, cleavage and blastocyst formation of Group 3 were higher than those of Group 1 and Group 2 (81.8% vs 33.3% vs 53.8%, 54.5% vs 16.7% vs 26.9%, p < 0.05; 15.2% vs 3.3% vs 7.7%, p > 0.05). The rabbit transplanted with embryos derived from Group 3 became pregnant. Embryos derived from double activation could implant into endometrium. Conclusion: Double activation may increase freeze–thawed oocytes developmental potential. After activation, oocytes cleavage velocity may be faster than that of oocytes without activation.

Keywords

ActivationIntracytoplasmic sperm injection (ICSI)OocytesRabbit

Information

Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 27 - 32
Copyright
Copyright © Cambridge University Press 2009

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