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Effects of oviductal fluid and heparin on fertility and characteristics of porcine spermatozoa

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Billy N. Day ,
Joon-Gyo Lim ,
Hoon Taek Lee  and
Kil Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Billy N. Day
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
Joon-Gyo Lim
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Hoon Taek Lee
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Kil Saeng Chung*
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
*
Dr Kil-Saeng Chung, Department of Animal Sciences, Kon-Kuk University, Kwangjin Gu, Mojin-dong, Seoul 133-701, Korea. Fax: 822-455-5305.
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Summary

The objective of this study was to determine the effects of oviductal fluid and heparin on sperm penetration and the characteristics of spermatozoa. The addition of oviductal fluid and heparin to the fertilisation medium decreased sperm penetration and the mean number of spermatozoa in penetrated eggs. The number of spermatozoa firmly bound to zona pellucida was also decreased in the presence of oviductal fluid and heparin. Chlortetracycline (CTC) fluorescence patterns were used to determine the incidence of capacitation and the acrosome reaction. The proportion of capacitated and acrosome-free spermatozoa increased when spermatozoa were exposed for 1.5 and 3 h to oviductal fluid and heparin. In contrast heparin alone did not increase the number of capacitated spermatozoa at these time points. These results suggest that factor(s) in oviductal secretions reduce polyspermic fertilisation and the number of spermatozoa that will penetrate porcine oocytes. The reduction of polyspermic penetration by oviductal secretions may be due to a reduced number of spermatozoa in the fertilisation medium with an intact acrosome.

Keywords

Acrosome reactionIn vitro fertilisationOviductal fluidPigPolyspermy
Type
Article
Information
Zygote , Volume 5 , Issue 1 , February 1997 , pp. 61 - 65
Copyright
Copyright © Cambridge University Press 1997

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