Hostname: page-component-7c8c6479df-nwzlb Total loading time: 0 Render date: 2024-03-28T09:52:48.471Z Has data issue: false hasContentIssue false

Efficiency of different incubation systems for the in vitro production of bovine embryos

Published online by Cambridge University Press:  12 September 2018

Camila M. Cavalcanti
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
Iana S. Campelo
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
Mirelly M.A.S. Silva
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
João V.S. Albuquerque
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
Luciana M. Melo
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
Vicente J.F. Freitas*
Affiliation:
Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil.
*
*All correspondence to: Vicente José de Figueirêdo Freitas. Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil. Tel: +55 8531019861. E-mail: vicente.freitas@uece.br

Summary

This study aimed to compare the efficiency of different incubation systems for in vitro embryo production in bovine. Oocytes/embryos were cultured in three incubators: conventional – CONV, mini bench – MINI and portable – PORT. After in vitro maturation (IVM), oocytes were verified for maturation rate. The remaining structures were submitted to in vitro fertilization and culture to verify cleavage (day 2) and blastocyst (day 7) rates. Reactive oxygen species (ROS) were evaluated in post-IVM oocytes and embryos (days 2 and 7) using arbitrary fluorescence units (AFUs). No significant difference (P>0.05) was observed for maturation rate. The CONV system (74.0%) produced the highest cleavage rate (P<0.05) when compared with PORT (59.5%), but similar (P>0.05) to MINI (65.0%). The same pattern and differences were observed for blastocyst rate: CONV (33.3%), MINI (32.3%) and PORT (21.9%). ROS levels were not different (P>0.05) in post-IVM oocytes: CONV (35.6±4.5), MINI (29.4±4.0) and PORT (35.6±4.5). For day-2 embryos, ROS levels were higher (P<0.05) in MINI (44.2±3.1) in comparison with CONV (27.7±3.7) and PORT (33.3±3.2). No significant difference (P>0.05) was observed in blastocysts. In conclusion, although it produced high ROS levels at day 2 of culture, the MINI system was as efficient as the CONV system for blastocyst production. This option may be an interesting and economical for the in vitro embryo industry.

Type
Research Article
Copyright
© Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ambrogi, M, Dall’Acqua, PC, Rocha-Frigoni, N, Leão, B Mingoti, GZ (2017) Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: effects on nuclear and cytoplasmic maturation and embryonic development in vitro . Reprod. Domest. Anim. 52, 409421.Google Scholar
Avery, B, Melsted, JK Greve, T (2000) A novel approach for in vitro production of bovine embryos: use of the oxoid atmosphere generating system. Theriogenology 54, 12591268.Google Scholar
Brackett, BG Oliphant, G (1975) Capacitation of rabbit spermatozoa in vitro . Biol. Reprod. 12, 260274.Google Scholar
Ciray, HN, Aksoy, T, Yaramanci, K, Karayaka, I Bahceci, M (2009) In vitro culture under physiologic oxygen concentration improves blastocyst yield and quality: a prospective randomized survey on sibling oocytes. Fertil. Steril. 91, 14591461.Google Scholar
Corrêa, GA, Rumpf, R, Mundim, T.C.D, Franco, MM Dode, MAN (2008) Oxygen tension during in vitro culture of bovine embryos: effect in production and expression of genes related to oxidative stress. Anim. Reprod. Sci. 104, 132142.Google Scholar
Diógenes, MN, Guimarães, A.L.S, Leme, LO, Maurício, MF Dode, MAN (2017) Effect of prematuration and maturation with fibroblast growth factor 10 (FGF10) on in vitro development of bovine oocytes. Theriogenology 102, 190198.Google Scholar
Fatehi, AN, Roelen, BA, Colenbrander, B, Schoevers, EJ, Gadella, BM, Beverst, MM van den Hurk, R (2005) Presence of cumulus cells during in vitro fertilization protects the bovine oocyte against oxidative stress and improves first cleavage but does not affect further development. Zygote 13, 177185.Google Scholar
Ferreira, CR, Saraiva, SA, Catharino, RR, Garcia, JS, Gozzo, FC, Sanvido, GB, Santos, LF, Lo Turco, EG, Pontes, JH, Basso, AC, Bertolla, RP, Sartori, R, Guardieiro, MM, Perecin, F, Meirelles, FV, Sangalli, JR Eberlin, MN (2010) Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 51, 12181227.Google Scholar
Fischer, B Bavister, BD (1993) Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J. Reprod. Fertil. 99, 673679.Google Scholar
Guemra, S, Santo, ES, Zanin, R, Monzani, PS, Sovernigo, TC, Ohashi, OM, Verde Leal, CL Adona, PR (2014) Effect of temporary meiosis block during prematuration of bovine cumulus–oocyte complexes on pregnancy rates in a commercial setting for in vitro embryo production. Theriogenology 81, 982987.Google Scholar
Guérin, P, El Mouatassim, S Menezo, Y (2001) Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175189.Google Scholar
Harvey, AJ, Kind, KL Thompson, JG (2002) REDOX regulation of early embryo development. Reproduction 123, 479486.Google Scholar
IETS (The International Embryo Transfer Society) (2015) Statistics of embryo collection and transfer in domestic farm animals. www.iets.org/pdf/comm_data/IETS_Data_Retrieval_2015_V2.pdf.Google Scholar
Khazaei, M Aghaz, F (2017) Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes. Int. J. Fertil. Steril. 11, 6369.Google Scholar
Lane, M (2001) Mechanisms for managing cellular and homeostatic stress in vitro . Theriogenology 55, 225236.Google Scholar
Lee, M, Grazi, R Seifer, D (2013) Incorporation of the K-Minc incubator and media system into the IVF lab: the future of IVF. J. Clin. Embryol. 13, 2132.Google Scholar
Marinho, LSR, Untura, RM, Morotti, F, Moino, LL, Rigo, AG, Sanches, BV, Pontes, JHF Seneda, MM (2012) Large-scale programs for recipients of in vitro-produced embryos. Anim. Reprod. 9, 323328.Google Scholar
Martín-Romero, FJ, Miguel-Lasobras, EM, Domínguez-Arroyo, JA, González-Carrera, E Álvarez, IS (2008) Contribution of culture media to oxidative stress and its effect on human oocytes. Reprod. Biomed. Online 17, 652661.Google Scholar
Mingoti, GZ, Castro, VSDC, Méo, SC, Barretto, L.S.S Garcia, JM (2011) The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. In Vitro Cell. Dev. Biol. - Animal 47, 361367.Google Scholar
Oliveira, CS, Saraiva, NZ, Cruz, MH, Mazeti, B, Oliveira, LZ, Lopes, FL Garcia, JM (2013) HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction 145, 917.Google Scholar
Pereira, MM, Machado, MA, Costa, FQ, Serapiao, RV, Viana, JH Camargo, LS (2010) Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reprod. Fertil. Dev. 22, 10741082.Google Scholar
Pontes, JHF, Nonato-Junior, I, Sanches, BV, Ereno-Junior, JC, Uvo, S, Barreiros, TRR, Oliveira, JA, Hasler, JF Seneda, MM (2009) Comparison of embryo yield and pregnancy rate between in vivo and in vitro methods in the same Nelore (Bos indicus) donor cows. Theriogenology 71, 690697.Google Scholar
Pontes, JH, Melo-Sterza, FA, Basso, AC, Ferreira, CR, Sanches, BV, Rubin, KC Seneda, MM (2010) Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology 75, 16401646.Google Scholar
Rinaudo, PF, Giritharan, G, Talbi, S, Dobson, AT Schultz, RM (2006) Effects of oxygen tension on gene expression in preimplantation mouse embryos. Fertil. Steril. 86, 12521265.Google Scholar
Sugiyama, S, McGowan, M, Phillips, N, Kafi, M Young, M (2007) Effects of increased ambient temperature during IVM and/or IVF on the in vitro development of bovine zygotes. Reprod. Domest. Anim. 42, 271274.Google Scholar
Thompson, JGE, Simpson, AC, Pugh, PA, Donnelly, PE Tervit, HR (1990) Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. J. Reprod. Fertil. 89, 573578.Google Scholar
Wang, F, Tian, XZ, Zhang, L, He, CJ, Ji, PY, Li, Y, Tan, DX Liu, GS (2014) Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization. Fertil. Steril. 101, 577586.Google Scholar
Yang, HW, Hwang, KJ, Kwon, HC, Kim, HS, Choi, KW Oh, KS (1998) Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum. Reprod. 13, 9981002.Google Scholar
Yuan, YQ, Van Soom, A, Coopman, F.O.J, Mintiens, K, Boerjan, ML, Van Zeveren, A, de Kruif, A Peelman, LJ (2003) Influence of oxygen tension on apoptosis and hatching in bovine embryos cultured in vitro . Theriogenology 59, 15851596.Google Scholar