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Eremomastax speciosa potentializes the PMSG-inducing effect on some physiological and biochemical parameters in PMSG-primed immature rats

Published online by Cambridge University Press:  12 August 2020

Gildas Tetaping Mbemya Open the ORCID record for Gildas Tetaping Mbemya [Opens in a new window] ,
Marie Stéphanie Goka Chekem ,
Landry Lienou Lienou ,
Njina Nguedia Sylvain Open the ORCID record for Njina Nguedia Sylvain [Opens in a new window] ,
Jiatsa Nathalie Donfack Open the ORCID record for Jiatsa Nathalie Donfack [Opens in a new window] ,
Denise Damasceno Guerreiro Open the ORCID record for Denise Damasceno Guerreiro [Opens in a new window] ,
Jacques Romain Njimou ,
Ana Paula Ribeiro Rodrigues Open the ORCID record for Ana Paula Ribeiro Rodrigues [Opens in a new window]  and
Phélix Bruno Telefo
Gildas Tetaping Mbemya*
Affiliation:
State University of Ceará, Faculty of Veterinary Sciences, LAMOFOPA, Fortaleza, CE, Brazil University of Dschang, Faculty of Science, Department of Biochemistry, URBPMAN, Dschang, Cameroon
Marie Stéphanie Goka Chekem
Affiliation:
University of Dschang, Faculty of Science, Department of Biochemistry, URBPMAN, Dschang, Cameroon
Landry Lienou Lienou
Affiliation:
University of Dschang, Faculty of Science, Department of Biochemistry, URBPMAN, Dschang, Cameroon
Njina Nguedia Sylvain
Affiliation:
University of Dschang, Faculty of Science, Department of Biochemistry, URBPMAN, Dschang, Cameroon
Jiatsa Nathalie Donfack
Affiliation:
Free University of Brussels, Research Laboratory on Human Reproduction, Brussels, Belgium
Denise Damasceno Guerreiro
Affiliation:
State University of Ceará, Faculty of Veterinary Sciences, LAMOFOPA, Fortaleza, CE, Brazil
Jacques Romain Njimou
Affiliation:
University of Ngaoundéré, School of Chemical Engineering and Mineral Industries, Ngaoundéré, Cameroon
Ana Paula Ribeiro Rodrigues
Affiliation:
State University of Ceará, Faculty of Veterinary Sciences, LAMOFOPA, Fortaleza, CE, Brazil
Phélix Bruno Telefo
Affiliation:
University of Dschang, Faculty of Science, Department of Biochemistry, URBPMAN, Dschang, Cameroon
*
Author for correspondence: Gildas Tetaping Mbemya. Programa de Pós-Graduação em Biotecnologia (PPGB-RENORBIO-UECE). Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Dr. Silas Munguba, 1700, Campus do Itaperi, Fortaleza – CE – Brasil. CEP: 60 714 903. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840/ URBPMAN, Department of Biochemistry, Faculty of Science, P.O. Box 67, UDs, Dschang, Cameroon. E-mail: gildasmbemya@yahoo.fr
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Summary

The present study evaluated the effect of the aqueous extract from leaves of E. speciosa on some physiological and biochemical parameters of reproduction and the onset of puberty in pregnant mare serum gonadotropin (PMSG)-primed immature female rats. High pressure liquid chromatography (HPLC) was used to quantify the phenolic compounds in the methanol/methylene chloride (1:1) extract, the ethanolic and ethyl acetate fractions and the aqueous residue of E. speciosa. E. speciosa (0, 8, 32 or 64 mg/kg) were administered for 15 days to 24 non-PMSG-primed and 24 primed rats with 0.01 IU of PMSG. At the end of the treatment period, animal were sacrificed and their body, ovarian, uterine weight, ovarian protein or cholesterol level, as well as data on puberty onset were recorded. Of the 16 polyphenolic compounds quantitatively revealed in the extracts and fractions of E. speciosa after HPLC analysis, quercetin, rutin, apigenin and eugenol were the most abundant. Non-primed rats showed a significant increase (P < 0.05) in the uterine relative weight at the dose of 8 mg/kg when compared with the other treatments. The uterine proteins and the ovarian cholesterol (P < 0.05), respectively, showed a reduction at doses of 64 mg/kg and 32 mg/kg in non-primed rats. However in PMSG-primed rats, a significant decrease (P < 0.05) was observed in ovarian cholesterol at 64 mg/kg. In conclusion, E. speciosa potentializes the PMSG-inducing effect on folliculogenesis in PMSG-primed rats.

Keywords

Eremomastax speciosaImmature female ratsInfertilityPMSGPotentialization
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 482 - 488
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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