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Expression of mRNA and protein localization of epidermal growth factor and its receptor in goat ovaries

Published online by Cambridge University Press:  01 May 2006

José R.V. Silva ,
Robert van den Hurk  and
José R. Figueiredo
José R.V. Silva*
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. Faculty of Veterinary Medicine, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
Robert van den Hurk
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
José R. Figueiredo
Affiliation:
Faculty of Veterinary Medicine, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
*
All correspondence to: J.R.V. Silva, Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, PPGCV, Av. Paranjana, 1700, CEP 60740-000, Fortaleza, CE, Brazil. Tel: +55 85 31019852. Fax: +55 85 31019859. e-mail: jroberto@uece.br
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Summary

To examine the possibility that epidermal growth factor (EGF) and its receptor (EGF-R) are expressed throughout folliculogenesis, we studied the presence and distribution of EGF and EGF-R in goat ovaries. Ovaries of goats were collected and either fixed in paraformaldehyde for immunohistochemical localization of proteins, or used for the isolation of follicles, luteal cells and ovarian surface epithelium to study mRNA expression for EGF and EGF-R, using the reverse transcriptase polymerase chain reaction. EGF protein and mRNA were found in primordial, primary and secondary follicles as well as in small and large antral follicles and in surface epithelium, but in corpora lutea only the protein could be detected. Antral follicles expressed EGF mRNA in oocyte, cumulus, mural granulosa and theca cells. For EGF-R, both protein and mRNA were present at all stages of follicular development and in all antral follicular compartments. EGF-R protein and mRNA were also found in corpora lutea and surface epithelium. It is concluded that EGF and its receptor are expressed in goat ovarian follicles at all stages of follicle development, in corpora lutea, and in ovarian surface epithelium.

Keywords

EGFEGF receptorFolliclesGoatOocyteOvary

Information

Type
Research Article
Information
Zygote , Volume 14 , Issue 2 , May 2006 , pp. 107 - 117
Copyright
Copyright © Cambridge University Press 2006

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