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Fertilization, hatching, and embryogenesis of diploid and triploid eggs of Anabas testudineus (Bloch, 1792)

Published online by Cambridge University Press:  09 October 2018

A. Hassan ,
V.T. Okomoda Open the ORCID record for V.T. Okomoda [Opens in a new window]  and
F.A.B. Sanusi
A. Hassan*
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria
V.T. Okomoda
Affiliation:
School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
F.A.B. Sanusi
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria
*
All correspondence to: A. Hassan. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture, P.M.B. 2373 Makurdi, Nigeria. Tel: +60199360392. E-mail: anuar@umt.edu.my
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Summary

This study investigated the breeding parameters and embryogenic development of diploid and heat shock-induced triploid eggs of Anabas testudineus (Bloch, 1792). To this effect, broodstocks of A. testudineus were induced to spawn using the Ovaprim® hormone. After fertilization, the eggs were divided into two groups and one portion heat shocked at 41°C (for 3 min), at approximately 4 min after fertilization. Results of fertilization, hatchability, as well as the sequence and timing of embryogenic development were collated from three breeding trials. Fertilization percentages were similar in both treatments (≈90%) while hatchability was higher in the diploid eggs (79.56%) than the triploid induced eggs (50.04%). Both treatments had the same sequence of embryogenetic stages; however, the timing of development was significantly delayed in the triploids (i.e. beyond the 2-cell stages) as compared with the observations in the control group (diploid eggs). Consequently, hatching time was 5 h faster in the diploid eggs [i.e. 18 hours post fertilization (hpf)] compared with the triploid induced eggs (23 hpf). The most critical stage of embryonic development in which mass mortality occurred in the different treatments was the somite stage. The status of triploid hatchlings was affirmed using erythrocyte morphology in 2-month-old fingerlings.

Keywords

Climbing perchEmbryogenesisErythrocyte morphologyHeat shockSpawning performance
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 343 - 349
Copyright
© Cambridge University Press, 2018 

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