No CrossRef data available.
Article contents
FGF18 modulates CTGF mRNA expression in cumulus–oocyte complexes and early bovine embryos: preliminary data
Published online by Cambridge University Press: 18 August 2021
Summary
The Hippo pathway is involved in the proliferation of intrafollicular cells and in early embryonic development, mainly because effectors of this pathway are key transcription regulators of genes such as CTGF and CYR61, which are involved in cell proliferation. Recent studies by our group found that fibroblast growth factor 18 (FGF18) is present in the fallopian tube during early embryonic development, leading to the hypothesis that FGF18 may have a role during embryonic development. Therefore, the aim of the following study was to determine whether FGF18 modulates the expression of Hippo pathway target genes, CTGF and CYR61, during oocyte maturation and early embryonic development. Three experiments were carried out, with in vitro maturation (IVM) of cumulus–oocyte complexes (COCs) and embryo culture. In experiment one, FGF18 (100 ng/ml) induced an increase (P < 0.05) in CTGF gene expression at 12 h post-exposure. In experiment two, FGF18 (100 ng/ml) induced a reduction (P < 0.05) in CTGF expression at 3 h post-exposure. In the third experiment, day 7 embryos exposed to FGF18 during oocyte IVM expressed greater CTGF mRNA abundance, whereas FGF18 exposure during embryo in vitro embryo culture did not alter CTGF expression in comparison with untreated controls. The preliminary data presented here show that FGF18 modulates CTGF expression in critical periods of oocyte nuclear maturation, cumulus expansion and early embryonic development in cattle.
Keywords
- Type
- Research Article
- Information
- Copyright
- © The Author(s), 2021. Published by Cambridge University Press
References
Boopathy, GTK and Hong, W (2019). Role of Hippo pathway–YAP/TAZ signaling in angiogenesis. Front Cell Dev Biol 7, 49.CrossRefGoogle ScholarPubMed
Böttcher, RT and Niehrs, C (2005). Fibroblast growth factor signaling during early vertebrate development. Endocr Rev 26, 63–77.CrossRefGoogle ScholarPubMed
da Silva, RB, Yang, MY, Caixeta, ES, Castilho, AC, Amorim, RL, Price, CA, Fortune, JE and Buratini, J (2019). Fibroblast growth factor 18 regulates steroidogenesis in fetal bovine ovarian tissue in vitro
. Mol Reprod Dev 86, 166–74.CrossRefGoogle ScholarPubMed
Higaki, S, Kishi, M, Koyama, K, Nagano, M, Katagiri, S, Takada, T and Takahashi, Y (2017). Early germinal vesicle breakdown is a predictor of high preimplantation developmental competent oocytes in mice. Zygote 25, 41–8.Google ScholarPubMed
Hyttel, P, Viuff, D, Fair, T, Laurincik, J, Thomsen, PD, Callesen, H, Vos, PL, Hendriksen, PJ, Dieleman, SJ, Schellander, K, Besenfelder, U and Greve, T (2001). Ribosomal RNA gene expression and chromosome aberrations in bovine oocytes and preimplantation embryos. Reproduction 122, 21–30.Google ScholarPubMed
Jiang, Z, Guerrero-Netro, HM, Juengel, JL and Price, CA (2013). Divergence of intracellular signaling pathways and early response genes of two closely related fibroblast growth factors, FGF8 and FGF18, in bovine ovarian granulosa cells. Mol Cell Endocrinol 375(1–2), 97–105.Google ScholarPubMed
Krupska, I, Bruford, EA and Chaqour, B (2015). Eyeing the Cyr61/CTGF/NOV (CCN) group of genes in development and diseases: Highlights of their structural likenesses and functional dissimilarities. Hum Genomics 9, 24.Google ScholarPubMed
Liu, Q, Zhang, J, Wen, H, Feng, Y, Zhang, X, Xiang, H, Cao, Y, Tong, X, Ji, Y and Xue, Z (2018). Analyzing the transcriptome profile of human cumulus cells related to embryo quality via RNA sequencing. Biomed Res Int 2018, 9846274.Google ScholarPubMed
Lorthongpanich, C and Issaragrisil, S (2015). Emerging role of the hippo signaling pathway in position sensing and lineage specification in mammalian preimplantation embryos. Biol Reprod 92, 143.Google ScholarPubMed
Ocón-Grove, OM, Cooke, FN, Alvarez, IM, Johnson, SE, Ott, TL and Ealy, AD (2008) Ovine endometrial expression of fibroblast growth factor (FGF) 2 and conceptus expression of FGF receptors during early pregnancy. Domest Anim Endocrinol 34, 135–45.CrossRefGoogle ScholarPubMed
Ohashi, S, Naito, K, Sugiura, K, Iwamori, N, Goto, S, Naruoka, H and Tojo, H (2003). Analyses of mitogen-activated protein kinase function in the maturation of porcine oocytes. Biol Reprod 68, 604–9.CrossRefGoogle ScholarPubMed
Pfaffl, MW (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acid Res 29, e45.CrossRefGoogle ScholarPubMed
Pincus, G and Enzmann, EV (1935). The comparative behavior of mammalian eggs in vivo and in vitro: I. The activation of ovarian eggs. J Exp Med 62, 665–75.Google ScholarPubMed
Portela, VM, Machado, M, Buratini, J Jr, Zamberlam, G, Amorim, RL, Goncalves, P and Price, CA (2010). Expression and function of fibroblast growth factor 18 in the ovarian follicle in cattle. Biol Reprod 83, 339–46.Google ScholarPubMed
Sánchez, F and Smitz, J (2012). Molecular control of oogenesis. Biochim Biophys Acta 1822, 1896–912.CrossRefGoogle ScholarPubMed
Sanz-Ezquerro, JJ, Münsterberg, AE and Stricker, S (2017). Editorial: Signaling pathways in embryonic development. Front Cell Dev Biol 5, 76.Google ScholarPubMed
Serrano, I, McDonald, PC, Lock, F, Muller, WJ and Dedhar, S (2013). Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase. Nat Commun 4, 2976.Google ScholarPubMed
Tian, XC, Lonergan, P, Jeong, BS, Evans, AC and Yang, X (2002). Association of MPF, MAPK, and nuclear progression dynamics during activation of young and aged bovine oocytes. Mol Reprod Dev 62, 132–8.CrossRefGoogle Scholar
Yu, FX and Guan, KL (2013). The Hippo pathway: Regulators and regulations. Genes Dev 27, 355–71.CrossRefGoogle ScholarPubMed