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Freezing injuries in the embryos of Piaractus mesopotamicus

Published online by Cambridge University Press:  23 August 2010

Darci Carlos Fornari ,
Ricardo Pereira Ribeiro ,
Danilo Pedro Streit Jr ,
Lauro Vargas ,
Nelson M. Lopera Barrero  and
Gentil Vanini de Moraes
Darci Carlos Fornari*
Affiliation:
Departamento Zootecnia–Universidade Estadual de Maringá, Av. Colombo, 5790-Campus Universitário, CEP: 87020-900 Maringá, Paraná, Brasil.
Ricardo Pereira Ribeiro
Affiliation:
Universidade Estadual de Maringá (UEM) – Depto. de Zootecnia–Maringá, Paraná State, Brasil.
Danilo Pedro Streit Jr
Affiliation:
Universidade Federal do Rio Grande do Sul – UFRGS, Brasil.
Lauro Vargas
Affiliation:
Universidade Estadual de Maringá (UEM) – Depto. de Zootecnia–Maringá, Paraná State, Brasil.
Nelson M. Lopera Barrero
Affiliation:
Universidade Estadual de Maringá (UEM) – Grupo de Pesquisa PeixeGen
Gentil Vanini de Moraes
Affiliation:
Universidade Estadual de Maringá (UEM) – Depto. de Zootecnia–Maringá, Paraná State, Brasil.
*
All correspondence to: Darci Carlos Fornari. Departamento Zootecnia–Universidade Estadual de Maringá, Av. Colombo, 5790-Campus Universitário, CEP: 87020-900 Maringá, Paraná, Brasil. Tel: +55 44 3261 8969. e-mail: darci.peixegen@gmail.com
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Summary

Cryopreservation of mammal embryos has been technically feasible for many years, but morphological injuries still persist in fish embryos during cryopreservation. Thus, the objective of the present study was to describe these freezing injuries in Piaractus mesopotamicus embryos. Two hundred and twenty-five embryos were collected at the post-gastrula stage and assigned into four treatments of sucrose at 8.5, 17.0, 25.0 or 34.0% plus 9.0% methanol. The control was prepared with distilled water only. The gradual decrease in the temperature was 0.5°C/min. After the seeding stage, the fish embryos were stored in liquid nitrogen at −33°C. Thereafter, they were thawed for evaluating per cent hatching, and the samples collected from every treatment were submitted to scanning electron microscopy for morphological analysis. The micrographic images showed that there was substantial alterations in embryo morphology under the highest concentrations of sucrose. These solutions did not prevent the formation of ice crystals, which lead to deformities and killed the embryos, but the observed reduced level of morphological structure in these embryos when treated with 17.0% sucrose plus 9.0% methanol is a compelling argument for additional studies.

Keywords

CryoprotectantMethanolMorphologicalPacuReproductionSucrose
Type
Research Article
Information
Zygote , Volume 19 , Issue 4 , November 2011 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 2010

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