Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-25T22:09:32.369Z Has data issue: false hasContentIssue false
  • English
  • Français

Interaction between embryos and culture conditions during in vitro development of bovine early embryos

Published online by Cambridge University Press:  01 May 2008

Yoshikazu Nagao ,
Rumi Iijima  and
Kazuhiro Saeki
Yoshikazu Nagao*
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi 321–4415, Japan.
Rumi Iijima
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi 321–4415, Japan.
Kazuhiro Saeki
Affiliation:
Research Institute of Biology-Oriented Science and Technology, Kinki University, Wakayama 649–6493, Japan.
*
All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. Tel/Fax: +81 285 84 1321. e-mail: ynagao@cc.utsunomiya-u.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

Various factors such as embryo density and substances in the medium can influence embryo development in vitro. These factors and the embryos probably interact with each other, however the interactions are not fully understood. To investigate the interactions, we examined the effects of the number of embryos, drop size, oxygen concentration and glucose and inorganic phosphate in the medium during protein-free culture of bovine IVM/IVF embryos. In Experiment 1, different numbers of embryos were cultured in a 50 μl drop of medium. The frequencies of blastocyst development in the groups with 25, 50 and 100 embryos per drop were higher than in the other groups. One, five and 25 embryos were cultured in different drop sizes (Experiment 2), a 50 µl drop of medium at different O2 concentrations (Experiment 3) and a 50 µl drop of medium excluding glucose and/or inorganic phosphate (Experiment 4). In Experiment 2, the size of the medium drops did not improve blastocyst development. In Experiment 3, the highest frequency of blastocyst development for one, five and 25 embryos per drop was obtained at 1, 2.5 and 5% O2, respectively. In Experiment 4, blastocyst development for one and five embryos per drop were improved in the medium excluded inorganic phosphate. These results indicate that there is a cooperative interaction among embryos during culture and that this interaction may be mediated by reduction of toxic factors in the medium. At low embryo density, reduced oxygen concentration or the exclusion of inorganic phosphate enhanced blastocyst development.

Keywords

Bovine embryoCulture mediumDensityDevelopmentOxygen
Type
Research Article
Information
Zygote , Volume 16 , Issue 2 , May 2008 , pp. 127 - 133
Copyright
Copyright © Cambridge University Press 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brackett, B.G. & Oliphant, G. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12, 260–74.CrossRefGoogle ScholarPubMed
Carolan, C., Lonergan, P., Khatir, H. & Mermillod, P. (1996). In vitro production of bovine embryos using individual oocytes. Mol. Reprod. Dev. 45, 145–50.3.0.CO;2-T>CrossRefGoogle ScholarPubMed
Corps, A.N., Brigstock, D.R., Littlewood, C.J. & Brown, K.D. (1990). Receptors for epidermal growth factor and insulin-like growth factor-I on preimplantation trophoderm of the pig. Development 110, 221–7.CrossRefGoogle ScholarPubMed
Fischer, B. & Bavister, B.D. (1993). Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J. Reprod. Fertil. 99, 673–9.CrossRefGoogle ScholarPubMed
Fujita, T., Umeki, H., Shimura, H., Kugumiya, K. & Shiga, K. (2006). Effects of group culture and ebbryo-culture conditioned medium on development of bovine embryos. J. Reprod. Dev. 52, 137–42.CrossRefGoogle ScholarPubMed
Gardner, H.G. & Kaye, P.L. (1991). Insulin increases cell numbers and morphorogical development in mouse pre-implantation embryos in vitro. Reprod. Fertil. Dev. 3, 79–91.CrossRefGoogle Scholar
Gopichandran, N. & Leese, H.J. (2006). The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction 131, 269–77.CrossRefGoogle ScholarPubMed
Houghton, F.D., Thompson, J.G., Kennedy, C.J. & Leese, H.J. (1996). Oxygen consumption and energy metabolism of the early mouse embryo. Mol. Reprod. Dev. 44, 476–85.3.0.CO;2-I>CrossRefGoogle ScholarPubMed
Ikeda, K., Takahashi, Y. & Katagiri, S. (2000). Effects of medium change on the development of in vitro matured and fertilized bovine oocytes cultured in medium containing amino acids. J. Vet. Med. Sci. 62, 121–3.CrossRefGoogle ScholarPubMed
Iritani, A. & Niwa, K. (1977). Capacitation of bull spermatozoa and fertilization in vitro of cattle follicular oocytes matured in culture. J. Reprod. Fertil. 50, 119–21.CrossRefGoogle ScholarPubMed
Kane, M.T. (1983). Variability in different lots of commercial bovine serum albumin affects cell multiplication and hatching of rabbit blastocysts in culture. J. Reprod. Fertil. 69, 555–8.CrossRefGoogle ScholarPubMed
Kane, M.T. & Headon, D.R. (1980). The role of commercial bovine serum albumin preparations in the culture of one-cell rabbit embryos to blastocysts. J. Reprod. Fertil. 60, 469–75.CrossRefGoogle ScholarPubMed
Kato, Y. & Tusnoda, Y. (1994). Effects of the culture density of mouse zygotes on the development in vitro and in vivo. Theriogenology 41, 1315–22.CrossRefGoogle ScholarPubMed
Keefer, C.L., Stice, S.L., Paprocki, A.M. & Golueke, P. (1990). In vitro culture of bovine IVM-IVF embryos: Cooperative interaction among embryos and the role of growth factors. Theriogenology 41, 1323–31.CrossRefGoogle Scholar
Lane, M. & Gardner, K. (1992). Effects of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum. Reprod. 17, 558–62.Google Scholar
Lee, E.S., Fukui, Y., Lee, B.C., Lim, J.M. & Hwang, W.S. (2004). Promoting effects of amino acids added to a chemically defined medium on blastocyst formation and blastomere proliferation of bovine embryos cultured in vitro. Anim. Reprod. Sci. 84, 257–67.Google Scholar
Mass, D.H.A., Storey, B.T. & Mastroianni, L. Jr. (1976). Oxygen tension in the oviduct of the rhesus monkey (Macaca mulatta). Fertil. Steril. 27, 1312–7.Google Scholar
Mastroianni, L. Jr. & Jones, R. (1965). Oxygen tension within the rabbit fallopian tube. J. Reprod. Fertil. 9, 99–102.CrossRefGoogle ScholarPubMed
Mills, R.M. & Brinster, R.L. (1967). Oxygen consumption of preimplantation mouse embryos. Exp. Cell. Res. 47, 337–44.CrossRefGoogle Scholar
Mizushima, S. & Fukui, Y. (2000). Fertilizability and developmental capacity of bovine oocytes cultured individually in a chemically defined maturation medium. Theriogenology 55, 1431–45.CrossRefGoogle Scholar
Nagao, Y., Saeki, K., Hoshi, M. & Kainuma, H. (1994). Effects of oxygen concentration and oviductal epithelial tissue on the development of in vitro matured and fertilized bovine oocytes cultured in protein-free medium. Theriogenology 41, 681–7.Google Scholar
Nagao, Y., Saeki, K., Hoshi, M. & Nagai, M. (1995). Early development of bovine embryos. J. Reprod. Dev. 41, j29–j36.CrossRefGoogle Scholar
Nagao, Y., Saeki, K., Hoshi, M., Takahashi, Y. & Kanagawa, H. (1995). Effects of water quality on in vitro fertilization and development of bovine oocytes in protein-free medium. Theriogenology 44, 433–44.CrossRefGoogle ScholarPubMed
Nasr-Esfhani, M.H., Aitken, J.R. & Jonson, M.H. (1990). Hydrogen peroxide levels in mouse oocytes and early cleavage stage embryos developed in vitro and in vivo. Development 109, 501–7.CrossRefGoogle Scholar
O'Neill, C. (1985). Partial characterisation of the embryo-derived platelet-activating factor in mice. J. Reprod. Fertil. 75, 375–80.CrossRefGoogle ScholarPubMed
O'Neill, C. (1997). Evidence for the requirement of autodrine growth factors for development of mouse preimplantation embryo in vitro. Biol. Reprod. 56, 229–37.CrossRefGoogle Scholar
Orsi, N.S. & Leese, H.J. (2004). Ammonium exposure and pyruvate affect the amino acid metabolism of bovine blastocyts in vitro. Reproduction 127, 131–40.Google Scholar
Paria, B.C. & Dey, S.K. (1990). Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. Proc. Natl. Acad. Sci. USA. 87, 4761–5.CrossRefGoogle ScholarPubMed
Petters, R.M., Johnson, B.H., Reed, M.L. & Archibong, A. E. (1990). Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. J. Reprod. Fertil. 89, 269–75.CrossRefGoogle ScholarPubMed
Pinyopummintr, T. & Bavister, B.D. (1991). In vitro-matured/in vitro-fertilized bovine oocytes can develop into morulae/blastocysts in chemically defined, protein-free culture media. Biol. Reprod. 45, 736–42.CrossRefGoogle ScholarPubMed
Quinn, P. & Harlow, G.M. (1978). The effect of oxygen on the development of preimplantation mouse embryos in vitro. J. Exp. Zool. 206, 73–80.CrossRefGoogle ScholarPubMed
Rappolee, D.A, Brenner, C.A., Schultz, R., Mark, D., & Werb, Z. (1988). Developmental expression of PDGF, TGF-α and TGF-β genes in preimplantation mouse embryos. Science 241, 1823–5.CrossRefGoogle ScholarPubMed
Rexroad, C.E. Jr. (1989). Co-culture of domestic animal embryos. Theirogenology 31, 105–14.CrossRefGoogle Scholar
Ribarov, S.R. & Benov, L.C. (1981). Relationship between the hemolytic action of heavy metals and lipid peroxidation. Biochem. Biophys. Acta 640, 721–26.Google Scholar
Saeki, K., Hoshi, M., Leibfried-Rutledge, M.L. & First, N.L. (1990a). In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone. Theriogenology 34, 1035–9.CrossRefGoogle Scholar
Saeki, K., Nagao, Y., Utaka, K. & Ishimori, H. (1990b). Maturation of bovine oocytes by co-culture with rabbit preovulatory follicle cells. Jpn. J. Zootech. Sci. 61, 89–90.Google Scholar
Salahuddin, S., Ookutsu, S., Goto, K., Nakanishi, Y. & Nagata, Y. (1995). Effects of embryo density and co-culture of unfertilized oocytes on embryonic development of in-vitro fertilized mouse embryos. Hum. Reprod. 10, 2382–5.CrossRefGoogle ScholarPubMed
Schini, S.A. & Bavister, B.D. (1988). Two-cell block to development of cultured hamster embryos is caused by inorganic phosphate and glucose. Biol. Reprod. 39, 1183–92.CrossRefGoogle ScholarPubMed
Seshagiri, P.B. & Bavister, B.D. (1989). Phosphate is required for inhibition by glucose of development of hamster 8-cell embryos in vitro. Biol. Reprod. 40, 607–14.CrossRefGoogle ScholarPubMed
Sirard, M.A., Parrish, J.J., Ware, C.B., Leibfried-Rutledge, M.L. & First, N.L. (1988). The culture of bovine oocytes to obtain developmentally competent embryos. Biol. Reprod. 39, 546–52.CrossRefGoogle ScholarPubMed
Thompson, J.G.E., Simpson, A.C., Pugh, P.A., Donnelly, P.E. & Tervit, H.R. (1990). Effects of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. J. Reprod. Fertil. 89, 573–8.CrossRefGoogle ScholarPubMed
Watson, A., Hogan, A., Hahnel, A., Wiemer, K.E. & Schultz, G.A. (1992). Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo. Mol. Reprod. Dev. 31, 87–95.Google Scholar
Yang, B.K., Yang, X. & Foote, R.H. (1993). Effects of growth factors on morula and blastocyst development of in vitro matured and in vitro fertilized bovine oocytes. Theriogenology 40, 521–30.CrossRefGoogle ScholarPubMed