Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 51
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Herrera, Carolina 2016. Clinical Applications of Preimplantation Genetic Testing in Equine, Bovine, and Human Embryos. Journal of Equine Veterinary Science, Vol. 41, p. 29.

    Ottolini, Christian S. Rogers, Shaun Sage, Karen Summers, Michael C. Capalbo, Antonio Griffin, Darren K. Sarasa, Jonas Wells, Dagan and Handyside, Alan H. 2015. Karyomapping identifies second polar body DNA persisting to the blastocyst stage: implications for embryo biopsy. Reproductive BioMedicine Online, Vol. 31, Issue. 6, p. 776.

    Palini, Simone De Stefani, Silvia Primiterra, Mariangela and Galluzzi, Luca 2015. Pre-implantation genetic diagnosis and screening: now and the future. Gynecological Endocrinology, Vol. 31, Issue. 10, p. 755.

    Bormann, Charles L. 2014. Yen & Jaffe's Reproductive Endocrinology.

    Montag, Markus Liebenthron, Jana and Köster, Maria 2014. A Practical Guide to Selecting Gametes and Embryos.

    Wang, Ze Nan Ang, Wei Tech Zhao, Su and Teo, Tat Joo 2014. 2014 13th International Conference on Control Automation Robotics & Vision (ICARCV). p. 1218.

    Ajduk, Anna and Zernicka-Goetz, Magdalena 2013. Quality control of embryo development. Molecular Aspects of Medicine, Vol. 34, Issue. 5, p. 903.

    Montag, M. Toth, B. and Strowitzki, T. 2013. Präimplantationsdiagnostik – PID. Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, Vol. 56, Issue. 12, p. 1670.

    Veiga, Anna Boiso, Irene and Belil, Itziar 2013. Textbook of Assisted Reproductive Technologies.

    Harper, Joyce C. and SenGupta, Sioban B. 2012. Preimplantation genetic diagnosis: State of the ART 2011. Human Genetics, Vol. 131, Issue. 2, p. 175.

    Thornhill, Alan R. Ottolini, Christian and Handyside, Alan H. 2012. Textbook of Assisted Reproductive Techniques, Volume One: Laboratory Perspectives.

    Veiga, Anna and Belil, Itziar 2012. Textbook of Assisted Reproductive Techniques, Volume One: Laboratory Perspectives.

    Harton, G. L. Magli, M. C. Lundin, K. Montag, M. Lemmen, J. and Harper, J. C. 2011. ESHRE PGD Consortium/Embryology Special Interest Group--best practice guidelines for polar body and embryo biopsy for preimplantation genetic diagnosis/screening (PGD/PGS). Human Reproduction, Vol. 26, Issue. 1, p. 41.

    Iwarsson, E. Malmgren, H. and Blennow, E. 2011. Preimplantation genetic diagnosis: twenty years of practice. Seminars in Fetal and Neonatal Medicine, Vol. 16, Issue. 2, p. 74.

    de Rycke, Martine and Sermon, Karen 2010. Molecular Diagnostics.

    Taylor, Tyl H. Gilchrist, Janice W. Hallowell, Susan V. Hanshew, Kelly K. Orris, John J. Glassner, Michael J. and Wininger, J. David 2010. The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage. Journal of Assisted Reproduction and Genetics, Vol. 27, Issue. 11, p. 663.

    De Vos and, Anick and Van Steirteghem, André 2009. Yen & Jaffe's Reproductive Endocrinology.

    Spits, Claudia and Sermon, Karen 2009. PGD for monogenic disorders: aspects of molecular biology. Prenatal Diagnosis, Vol. 29, Issue. 1, p. 50.

    Lanzendorf, Susan E. Ratts, Valerie S. Moley, Kelle H. Goldstein, Jerald S. Dahan, Michael H. and Odem, Randall R. 2007. A randomized, prospective study comparing laser-assisted hatching and assisted hatching using acidified medium. Fertility and Sterility, Vol. 87, Issue. 6, p. 1450.

    Mukaida, Tetsunori and Takahashi, Katsuhiko 2007. Vitrification in Assisted Reproduction.


Laser blastocyst biopsy for preimplantation diagnosis in the human

  • A. Veiga (a1), M. Sandalinas (a1), M. Benkhalifa (a1), M. Boada (a1), M. Carrera (a1), J. Santaló (a1), P.N. Barri (a1) and Y. Ménézo (a1)
  • DOI:
  • Published online: 01 September 2008

A new methodology for blastocyst biopsy that uses a 1.48μm in diode laser is described. Trophectoderm cells are biopsied after laster zona drilling and culture, fixed and processed for fluorescent in situ hybridisation (FISH) analysis. Preliminary results on the efficiency of the procedure and blastocyst recovery rate are promising. Blastocyst laser biopsy is a useful tool in preimplantation genetic diagnosis (PGD) as it allows a more reliable diagnosis and widens the diagnostic possibilities on account of the higher number of cells obtained in the biopsy.

Corresponding author
A. Veiga, Reproductive Medicine Service, Department of Obstetrics and Gynaecology, Institut Universitari Dexeus, Barcelona, Spain.
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

M. Benkhalifa , Y. Ménézo , L. Janny , et al., (1996). Cytogenetics of uncleaved oocytes and arrested zygotes in IVF programmes. J. Assist. Reprod. Genet. 13, 140–8.

S.A. Carson , W.L. Gentry & A.L. Smith (1993). Trophectoderm microbiopsy in murine blastocysts: comparison of four methods. J. Assist. Reprod. Genet. 10, 427–33.

I. Findlay , P. Ray , P. Quirke , A. Rutherford & R. Lilford (1995). Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis. Mol. Hum. Reprod. 10, 1609–18.

M. Germond D. Nocera , A. Senn , K. Rink , G. Delacrétaz , T. Pedrazzini & J.P. Hornung (1996). Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 μm diode laser beam. Hum. Reprod. 11, 1043–8.

A. Handyside , E. Kontogianni , K. Hardy (1990). Pregnancies from biopsied human preimplantation embryos sexed with a Y-specific DNA amplification. Nature 344, 768–70.

A. Handyside , J. Lesko , J. Tarín , R. Winston & M. Hughes (1992). Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis. N. Eng. J. Med. 327, 905–9.

L. Janny & Y. Ménézo (1994). Evidence of a strong paternal effect on human preimplantation embryo development and blastocyst formation. Mol. Reprod. Dev. 38, 3642.

L. Janny & Y. Ménézo (1996). Maternal age effect on early human embryonic development and blastocyst formation. Mol. Reprod. Dev. 45, 31–7.

Y. Ménézo & M. Benkhalifa (1995). Cytogenetics and cryobiology of human cocultured embryos: a 3 year experience. J. Assist. Reprod. Genet. 12, 3540.

Y. Ménézo , A. Hazout , M. Dumont , (1992). Coculture of embryos on Vero cells and transfer at the blastocyst stage. Hum. Reprod. 7 (Suppl. 1), 101–6.

S. Munné , H.U.G. Weier , J. Grifo & J. Cohen (1994). Chromosome mosaicism in human embryos. Biol. Reprod. 51, 373–9.

K. Rink , C. Delacrétaz , R.P. Salathé , A. Senn , D. Nocera , M. Germond , P. De Grandi & S. Fakan (1996). Non-contact microdrilling of mouse zona pellucida with an objective delivered 1.48 μm diode laser. Laser Surg. Med. 18, 5262.

A. Veiga , M.J. Torelló , M. Sandalinas , A. Busquets , G. Calderon & P.N. Barri (1995). Optimization of implantation in the in-vitro fertilization laboratory. Hum. Reprod. 10 Suppl. 2. 98106.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
Please enter your name
Please enter a valid email address
Who would you like to send this to? *