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Linolenic acid improves oocyte developmental competence and decreases apoptosis of in vitro-produced blastocysts in goat

Published online by Cambridge University Press:  20 November 2015

Arash Veshkini ,
Ali Akbar Khadem ,
Abdollah Mohammadi-Sangcheshmeh ,
Ali Asadi Alamouti ,
Masoud Soleimani  and
Eduardo L. Gastal
Arash Veshkini
Affiliation:
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran. Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran.
Ali Akbar Khadem
Affiliation:
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
Abdollah Mohammadi-Sangcheshmeh*
Affiliation:
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Theran P.O.Box: 11365/7117, Iran. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
Ali Asadi Alamouti
Affiliation:
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
Masoud Soleimani
Affiliation:
Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
Eduardo L. Gastal
Affiliation:
Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA.
*
All correspondence to: Abdollah Mohammadi-Sangcheshmeh. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Theran P.O.Box: 11365/7117, Iran. Fax: +98 21 36040907. E-mail: amohammadis@ut.ac.ir
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Summary

The effects of α-linolenic acid (ALA) on developmental competence of oocytes in goats were evaluated in this study. Initially, the level of ALA in small and large antral follicles was determined to be in a range of 0.018–0.028 mg/ml (64.6–100.6 μM, respectively). In vitro maturation was performed in the presence of various concentrations (10, 50, 100, or 200 μM) of ALA. Cumulus expansion, meiotic maturation, levels of intracellular glutathione (GSH), embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), number of total and apoptotic cells in blastocyst, and expression of Bax, Bcl-2, and p53 genes in blastocyst cells were determined. Compared with the control, no improvement was observed in cumulus expansion in ALA-treated groups. At 50 μM concentration, ALA increased meiotic maturation rate but had no effect on GSH level. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher rate of blastocyst formation was observed, and these embryos had a higher total cell number and a lower apoptotic cell number. Expression analyses of genes in blastocysts revealed lesser transcript abundances for Bax gene, and higher transcript abundances for Bcl-2 gene in 50 μM ALA group. Expression of p53 gene was also less observed in ALA-treated blastocysts. Our results show that ALA treatment at 50 μM during in vitro maturation (IVM) had a beneficial effect on maturation of goat oocytes and this, in turn, stimulated embryonic development and regulated apoptotic gene expression.

Keywords

Apoptosis genesBlastocystGSH levelGoat oocyteα-Linolenic acid
Type
Research Article
Information
Zygote , Volume 24 , Issue 4 , August 2016 , pp. 537 - 548
Copyright
Copyright © Cambridge University Press 2015 

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