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Lipid peroxidation in bull semen influences sperm traits and oxidative potential of Percoll®-selected sperm

Published online by Cambridge University Press:  05 April 2021

Vivian Cardoso Castiglioni Open the ORCID record for Vivian Cardoso Castiglioni [Opens in a new window] ,
Adriano Felipe Perez Siqueira ,
Luana de Cássia Bicudo ,
Tamie Guibu de Almeida ,
Thais Rose dos Santos Hamilton Open the ORCID record for Thais Rose dos Santos Hamilton [Opens in a new window] ,
Leticia Signori de Castro ,
Camilla Mota Mendes ,
Marcílio Nichi ,
João Diego de Agostini Losano Open the ORCID record for João Diego de Agostini Losano [Opens in a new window]  and
José Antônio Visitin
...Show all authors
Vivian Cardoso Castiglioni
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Adriano Felipe Perez Siqueira
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Luana de Cássia Bicudo
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Tamie Guibu de Almeida
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Thais Rose dos Santos Hamilton
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Leticia Signori de Castro
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Camilla Mota Mendes
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Marcílio Nichi
Affiliation:
Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
João Diego de Agostini Losano
Affiliation:
Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
José Antônio Visitin
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
Mayra Elena Ortiz D´Ávila Assumpção*
Affiliation:
Laboratory of Sperm Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
*
Author for correspondence: Mayra Elena Ortiz D´Ávila Assumpção Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87-05508-270, São Paulo, Brazil. Tel: +55 11 30917665. Fax: +55 11 30917412. Email: meoaa@usp.br
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Summary

Although bovine embryo in vitro production (IVP) is a common assisted reproductive technology, critical points warrant further study, including sperm traits and oxidative status of sperm for in vitro fertilization (IVF). Our aim was to evaluate whether the lipid peroxidation index of commercial bull semen is influenced by sperm traits and oxidative status of sperm populations selected using Percoll® gradient. Semen straws from 48 batches from 14 Nelore bulls were thawed individually, analyzed for motility and subjected to Percoll selection. After Percoll, the lipid peroxidation index of the extender was evaluated, whereas selected sperm were analyzed for motility, acrosome and membrane integrity, mitochondrial membrane potential, chromatin resistance and oxidative potential under IVF conditions. Batches were divided retrospectively in four groups according to lipid peroxidation index. Sperm from Group 4 with the lowest index of lipid peroxidation had, after Percoll selection, greater plasma membrane integrity (81.3%; P = 0.004), higher mitochondrial potential (81.1%; P = 0.009) and lower oxidative potential (135.3 ng thiobarbituric acid reactive substances (TBARS)/ml; P = 0.026) compared with Group 1 with highest lipid peroxidation index (74.3%, 73% and 213.1 ng TBARS/ml, respectively). Furthermore, we observed negative correlations for the lipid peroxidation index with motility, membrane integrity and mitochondrial potential, and positive correlations with oxidative potential. In conclusion, oxidative stress in semen straws, as determined using lipid peroxidation in the extender, is associated with sperm traits and their oxidative potential under IVF conditions. These results provided further insights regarding the importance of preventing oxidative stress during semen handling and cryopreservation, as this could affect sperm selected for IVF. Finally, Percoll selection did not completely remove sperm with oxidative markers.

Keywords

Bull spermOxidative stressSperm traitsTBARS
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 476 - 483
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors have contributed equally to this work.

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