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The microenvironment created by non-blocking embryos in aggregates may rescue blocking embryos via cell–embryo adherent contacts

Published online by Cambridge University Press:  26 September 2008

Galina G. Sekirina  and
Irina E. Neganova
Galina G. Sekirina*
Affiliation:
Laboratory of cell Morphology, Institute of CytologyRussian Academy of Sciences, Saint Petersburg, Russia
Irina E. Neganova
Affiliation:
Laboratory of cell Morphology, Institute of CytologyRussian Academy of Sciences, Saint Petersburg, Russia
*
Dr Galina G. Sekirina, RAS Institute of Cytology, 4 Tikhoretzky pr., Sankt-Petersburg 194064, Russia. Telephone: (812) 156-3322. Fax: (812) 247-0341.
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Summary

Under our culture conditions, mouse embryos from the BALB/c inbred mouse strain develop successfully in culture only from the late 2-cell stage onwards (so-called 2-cell block), whether or not EDTA is added to the culture medium. (CBA × C57BL) F2 embryos do not exhibit a 2-cell block. Medium conditioned by culture of non-blocking embryos from the 2-cell to the 8-cell stage did not improve the development of blocking embryos, nor did co-culture of blocking and non-blocking embryos, with or without conditioned medium. On the other hand phytohaemagglutinin (PHA)-assisted aggregation of an early 2-cell BALB/c embryo with five surrounding non-blocking F2 embryos (2-cell or 8-cell) or five BALB/c 8-cell embryos allowed the early 2-cell BALB/c embryos to develop into blastocysts within 72 h. Aggregation of blocking BALB/c 2-cell embryos with each other had no ‘rescue’ effect. When blocking and non-blocking 2-cell embryos were aggregated together, an integrated blastocyst was formed; but when the early 2-cell BALB/c embryos were aggregated with non-blocking 8-cell embryos, the blocking embryos formed a separate small blastocyst, which nonetheless retained adherent contact with the non-blocking embryos throughout the culture period. Ultrastructural analysis showed that 2-cell embryos aggregated with the aid of PHA form close adherent cell contacts up to several micrometres in length.

Keywords

Aggregates of embryos/chimerasCulture in vitroG2/M 2-cell blockMouse embryos
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 313 - 324
Copyright
Copyright © Cambridge University Press 1995

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