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Modulation of phosphatidylinositol 3-kinase activity during in vitro oocyte maturation increases the production of bovine blastocysts

Published online by Cambridge University Press:  03 August 2020

Janaína Leite Pereira ,
Alinne Glória Curcio ,
Laura Mathias Barroso ,
Edgar Mauricio Mogollón-Waltero ,
Helga Fernandes Gomes ,
Roger Cardoso Maia ,
Kelen Salaroli Viana ,
Maria Clara Caldas Bussiere Open the ORCID record for Maria Clara Caldas Bussiere [Opens in a new window] ,
Diego Fernando Dubeibe Marin  and
Angelo José Burla Dias Open the ORCID record for Angelo José Burla Dias [Opens in a new window]
Janaína Leite Pereira
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Alinne Glória Curcio
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Laura Mathias Barroso
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Edgar Mauricio Mogollón-Waltero
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Helga Fernandes Gomes
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Roger Cardoso Maia
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Kelen Salaroli Viana
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Maria Clara Caldas Bussiere
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Diego Fernando Dubeibe Marin
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
Angelo José Burla Dias*
Affiliation:
Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil
*
Author for correspondence: Angelo José Burla Dias. Avenida Alberto Lamego, 2000. CEP: 28013-602. Campos dos Goytacazes, Rio de Janeiro, Brazil. Tel: +55 22 27397285. E-mail: angeloburlauenf@gmail.com
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Summary

This study aimed to evaluate the effect of regulating phosphatidylinositol 3-kinase (PI3K) activity on the kinetics of oocyte nuclear maturation and the blastocyst rate. To evaluate oocyte viability, nuclear maturation rate and in vitro embryo production, cumulus–oocyte complexes (COCs) were maintained for 0, 10 min, 6 h or 22 h in TCM 199 medium supplemented with 20 nM wortmannin, an inhibitor of PI3K. After each period, COCs were transferred to the same medium without wortmannin and kept under the same conditions until completion of 22 h of in vitro maturation (IVM). To evaluate the effect of time on progression of nuclear maturation, COCs cultivated with 20 nM wortmannin was maintained for 22, 28 or 34 h of IVM. To determine the effect of wortmannin on the activity of maturation-promoting factor (MPF), COCs were kept under IVM conditions in the presence of the inhibitor for 0, 1, 3, 6, or 8 h. Exposure of COCs to wortmannin decreased (P < 0.05) the percentage of oocytes that reached metaphase II (MII) up to 22 h, MPF activity and reduced PI3K activity by 30%. However, after 28 and 34 h, 70% of oocytes reached the MII stage in the presence of inhibitor Moreover, COCs matured in the presence of wortmannin showed an increase (P < 0.05) in the blastocyst rate. These findings suggested that the regulation of the PI3K activity during IVM of bovine COCs interfered with the meiotic progression due to control of MPF activity, positively affecting the blastocyst rate.

Keywords

EmbryoKinasesMeiotic progressionMPFPI3K/Akt pathway
Type
Research Article
Information
Zygote , Volume 28 , Issue 5 , October 2020 , pp. 371 - 376
Copyright
© Cambridge University Press 2020

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