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Morphometric of blastomeres in Salmo salar

  • Brian R. Effer (a1), Rubén R. Sánchez (a2), Andrea M. Ubilla (a2), Elías V. Figueroa (a2) and Iván I. Valdebenito (a2)...
Summary

For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of S. salar. From a pool of eggs incubated at 7.5°C, 100 microphotographs of blastodiscs were extracted and analyzed at different incubation periods: 12, 14, 16, 20 or 24 h. Blastodiscs were characterized morphologically after 16, 20 or 24 h incubation, and classified into symmetric or asymmetric groups according to their morphology. The ratio of length (L) versus width (W) of each blastomere was determined, to establish its symmetry. In addition, 20 microphotographs of blastodiscs of normal appearance were analysed morphologically (control blastodisc: CB) for comparison (20 or 24 h). Results show that the first cleavage ends after 16 h of development. Seven categories were established during blastomere characterization: 47% normal (G1); 27% with dispersed margins (G2); 10% unequal (G3); 9% ‘pie-shaped’ (G4); 3% amorphous (G5); 2% three equal blastomeres and one different one (G6); and 2% with eccentric cleavage (G7). Although the incidence of abnormal cleavage in S. salar is uncertain, there is a potential for some asymmetries to be corrected during embryogenesis to generate viable individuals. More studies are necessary to correlate these abnormal cleavage patterns with indicators of quality in the later stages of embryogenesis in this species, to establish a quality assessment tool for gametes and/or embryos in salmonid species.

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Corresponding author
All correspondence to: Brian R. Effer. Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile. E-mail: breeiky@hotmail.com
References
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Akiyama, M., Tero, A. & Kobayashi, R. (2010). A mathematical model of cleavage. J. Theor. Biol. 264, 8494.
Avery, T. & Brown, J. (2005). Investigating the relationship among abnormal patterns of cell cleavage, egg mortality and early larval condition in Limanda ferruginea. J. Fish Biol. 67, 890–6.
Avery, T., Killen, S. & Hollinger, T. (2009). The relationship of embryonic development, mortality, hatching success, and larval quality to normal or abnormal early embryonic cleavage in Atlantic cod, Gadus morhua. Aquaculture 289, 265–73.
Arrau, J., Roblero, L. & Cury, M. (1981). New observations on the onset and duration of the meiotic prophase in the female golden hamster (Mesocricetus auratus). J. Anat. 132, 627–33.
Balinsky, B. (1983). Introducción a la Embriología. Ediciones Omega S.A. Barcelona, 736 pp.
Ballard, W. (1973). Morphogenetic movements in Salmo gairdneri Richardson. J. Exp. Zool. 184, 2748.
Ballard, W. (1985). Normal embryonic stages for salmonid fishes, base don Salmo gairdneri Richardson and Salvelinus fortinalis (Mitchill). J. Exp. Zool. 184, 726.
Billard, R. (1990). Culture of salmonids in fresh water. In Aquaculture vol. 2. (ed. Barnabé, G.). Ellis Horwood, England, pp. 549627.
Bobe, J. & Labbé, C. (2010). Egg and sperm quality in fish. Gen. Comp. Endocr. 165, 535–48.
Bromage, N., Bruce, M., Basavaraja, N. & Rana, K. (1994). Egg quality determinants in finfish: the role of overripening with special reference to the timing of stripping in the Atlantic halibut Hippoglossus hippoglossus. J. World Aquacult. Soc. 25, 1321.
Effer, B., Sanchez, R., Ubilla, A., Figueroa, E. & Valdebenito, I. (2012). Study of the first blastomeres in coho salmon (Oncorhynchus kisutch). Zygote Epub ahead of print doi: 10.1017/S0967199412000202
Giménez, G., Estévez, A., Lahnsteiner, F., Zecevic, B., Bell, J., Henderson, R., Piñera, J. & Sanchez-Prado, J. (2006). Egg quality criteria in common dentex (Dentex dentex). Aquaculture 260, 232–43.
Gorodilov, Y. (1996). Description of the early ontogeny of the Atlantic salmon, Salmon salar, with a novel system of interval (state) identification. Environ. Biol. Fish. 47, 109–27.
Hamoutene, D., Lush, L., Drover, D. & Walsh, A. (2009). Investigation of the temporal effects of spawning season and maternal and parental differences on egg quality in Atlantic cod Gadus morhua L. broodstock. Aquac. Res. 40, 1668–79.
Killeen, J., McLay, H. & Johnston, I. (1999). Development in Salmo trutta at different temperatures, with a quantitative scoring method for intraspecific comparisons. J. Fish Biol. 55, 382404.
Kjørsvik, E., Mangor-Jensen, A. & Holmefjord, I. (1990). Egg quality in fishes. Adv. Mar. Biol. 26, 71113.
Kjørsvik, E., Hoehne-Reitan, K. & Reitan, K.I. (2003). Egg and larval quality criteria as predictive measures for juvenile production in turbot (Scophthalmus maximus L.). Aquaculture 227, 920.
Kopsch, Fr. (1911). Die Entstehung des Dottersackentoblast und die Furchung bei der Forelle (Salmo fario). Archiv für Mikroskopische Anatomie 78, 618–59.
Kunz, Y. (2004). Developmental Biology of Teleost Fishes. Springer, The Netherlands, 638 pp.
Moran, D., Smith, C., Gara, B. & Poortenaar, C.W. (2007). Reproductive behaviour and early development in yellowtail kingfish (Seriola lalandi Valenciennes 1833). Aquaculture 262, 95104.
Pavlov, D. & Emel′yanova, N., (2008). Morphological criteria of egg quality in marine fishes: activation and cleavage of eggs of Zebrasoma scopas (Acanthuridae). J. Ichthyology 48, 533–48.
Pavlov, D. & Moksness, E. (1994). Reproductive biology, early ontogeny, and effect of temperature on development in wolfish: comparison with salmon. Aquacult. Int. 2, 133–53.
Penney, R., Lush, P., Wade, J., Brown, J., Parrish, C. & Burton, M. (2006). Comparative utility of egg blastomere morphology and lipid biochemistry for prediction of hatching success in Atlantic cod, Gadus morhua L. Aquac. Res. 37, 272–83.
Rani, M. (2005). Prediction of Larval Viability Based on Egg Quality Parameters and Early Cleavage Patterns in the Experiments of Triploidy Induction in Atlantic Cod, Gadus morhua L. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in International Fisheries Management. 64 pp. Norwegian College of Fishery Science, University of Tromso-Norway.
Shields, R., Brown, N. & Bromage, N. (1997). Blastomere morphology as a predictive measure of fish egg viability. Aquaculture 155, 112.
Sink, T. & Lochmann, R. (2008). Effects of dietary lipid source and concentration on canned catfish (Ictalurus punctatus) egg biochemical composition, egg and fry production, and egg and fry quality. Aquaculture 283, 6876.
Valdebenito, I., Sanchez, R., Effer, B. & Ubilla, A. (2012). Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss). Zygote 20, 327–31.
Vassallo-Agius, R., Watanabe, T., Yoshizaki, G., Satoh, S. & Takeuchi, Y. (2001). Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs, semen and livers. Fish. Sci. 67, 818–27.
Vásquez, P., Llanos-Rivera, A. & Castro, L. (2010). Anormalidades durante el desarrollo embrionario de sardina común, Strangomera bentincki en el ambiente natural. Rev. Biol. Mar. Oceanogr. 45, 177–85.
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Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
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