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Oviductal and endometrial mRNA expression of implantation candidate biomarkers during early pregnancy in rabbit

Published online by Cambridge University Press:  29 November 2013

Ayman Moustafa Saeed ,
María de los Desamparados Saenz de Juano ,
Francisco Marco Jiménez  and
José Salvador Vicente
Ayman Moustafa Saeed
Affiliation:
Animal Production Research Institute, Animal Biotechnology Department. Giza, Egypt.
María de los Desamparados Saenz de Juano
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, 46022-Valencia, Spain.
Francisco Marco Jiménez
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, 46022-Valencia, Spain.
José Salvador Vicente*
Affiliation:
Reproduction Biotechnology Laboratory, Institute of Science and Animal Technology (ICTA) at the Polytechnic University of Valencia, C/Camino de Vera s/n, 46022 Valencia, Spain Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, 46022-Valencia, Spain.
*
All correspondence to: José Salvador Vicente. Reproduction Biotechnology Laboratory, Institute of Science and Animal Technology (ICTA) at the Polytechnic University of Valencia, C/Camino de Vera s/n, 46022 Valencia, Spain. Tel: +34 96 3879754. Fax: +34 96 3877439. e-mail address: jvicent@dca.upv.es
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Summary

Prenatal losses are a complex problem. Pregnancy requires orchestrated communication between the embryo and the uterus that includes secretions from the embryo to signal pregnancy recognition and secretion and remodelling from the uterine epithelium. Most of these losses are characterized by asynchronization between embryo and uterus. To better understand possible causes, an analysis was conducted of gene expression of a set of transcripts related to maternal recognition and establishment of rabbit pregnancy (uteroglobin, SCGB1A1; integrin α1, ITGA1; interferon-γ, IFNG; vascular endothelial growth factor, VEGF) in oviduct and uterine tissue at 16, 72 or 144 h post-ovulation and insemination. In the oviduct tissue, a significant decrease in the level of SCGB1A1 mRNA expression was observed from 144 h post-ovulation. In the case of ITGA1, the transcript abundance was initially lower, but mRNA expression increased significantly at 72 and 144 h post-ovulation. For IFNG, a huge decrease was observed from 16 to 72 h post-ovulation. Finally, no significant differences were observed in the VEGF transcript. For the endometrium, the results showed a significant decline in the level of SCGB1A1 mRNA expression from 16 to 144 h post-ovulation induction. The highest levels of ITGA1 transcript were detected at 144 h, followed by the 16 h group and lower at 72 h post-ovulation. For IFNG there were no significant differences among post-ovulation induction times. Finally, it was possible to observe that VEGF mRNA abundance was present at low levels at 16 h post-ovulation and remained low at 72 h, but increased at 144 h. The functional significance of these observations may provide new insights into the maternal role in prenatal losses.

Keywords

EndometriumImplantation biomarkersOviductRabbit
Type
Research Article
Information
Zygote , Volume 23 , Issue 2 , April 2015 , pp. 288 - 296
Copyright
Copyright © Cambridge University Press 2013 

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