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Production of buffalo embryos using oocytes from in vitro grown preantral follicles

Published online by Cambridge University Press:  01 February 2008

P.S.P. Gupta ,
H.S. Ramesh ,
B.M. Manjunatha ,
S. Nandi  and
J.P. Ravindra
P.S.P. Gupta*
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
H.S. Ramesh
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
B.M. Manjunatha
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
J.P. Ravindra
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
*
1All correspondence to: P.S.P. Gupta, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India. E-mail: pspgupta2001@yahoo.co.in; pspgupta@hotmail.com
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Summary

The present study examines the use of buffalo preantral follicles as a source of oocytes for in vitro embryo production. Preantral follicles were isolated from abattoir-derived buffalo ovaries and were grown for 100 days in five different culture systems: (1) minimum essential medium (MEM); (2) coconut water; (3) MEM + ovarian mesenchymal cell (OMC) co-culture; (4) MEM + granulosa cell (GC) co-culture; or (5) MEM + cumulus cell (CC) co-culture. Low growth rates for the preantral follicles were observed when follicles were cultured in MEM or coconut water medium. Moderate growth rates were seen for OMC and GC co-cultures, and high rates of growth were observed when follicles were grown in CC co-culture. The survival of preantral follicles was low in the MEM culture (<25%), but was over 75% in the other culture systems. Oocytes were not recovered from the MEM group, while an oocyte recovery rate of 80–100% was observed when the follicles were cultured with coconut water/somatic cells. Transferable embryos could be produced only with the oocytes obtained from preantral follicles grown in the OMC and CC co-culture systems. This study demonstrates, for the first time, that it is possible to produce buffalo embryos by in vitro fertilization of oocytes derived from in vitro grown preantral follicles.

Keywords

BuffaloEmbryoIn vitro culturePreantral follicleSomatic cells
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 57 - 63
Copyright
Copyright © Cambridge University Press 2008

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References

Aboul-Ela, M.B.E. (2000). Superovulation in the buffaloes: constraints and manipulation. Buffalo J. 16, 1–20.Google Scholar
Andrade, E.R., Amorim, C.A., Matos, M.H.T., Rodrigues, A.P.R., Silva, J.R.V., Dode, M.A.N. & Figueiredo, J.R. (2002). Evaluation of saline and coconut water solutions in the preservation of sheep preantral follicles in situ. Small Rumin. Res. 43, 235–43.Google Scholar
Andrade, E.R., Seneda, M.M., Alfieri, A.A., Oliveira, J.R., Figueiredo, R. & Toniolli, I. (2005). Effect of indol acetic acid concentration in the activation and growth of ovine preantral follicles. Arq. Bras. Med. Vet. Zootec. 57, 334–39.CrossRefGoogle Scholar
Blume, H., Vale-Filho, V.R., Marques, A.P. Jr & Saturnino, H.M. (1988). Evaluation of coconut water for bovine embryo culture. Arq. Bras. Med. Vet. Zootec. 50, 395–9.Google Scholar
Cecconi, S., Barboni, B., Coccia, M. & Mattioli, M. (1999). In vitro development of sheep preantral follicles. Biol. Reprod. 60, 594–601.CrossRefGoogle ScholarPubMed
Combarnous, Y. & Nunes, F. J. (1995). Sperm extender including indole derivative. International patent no. 2725342: PCT/FR95/01322(FR 94-12122).Google Scholar
Costa, S.H.F., Santos, R.R., Ferreira, M.A.L., Machado, V.P., Rodrigues, A.P.R., Ohashi, O.M. & Figueiredo, J.R. (2002) Preservation of goat preantral follicles in saline or coconut water solution. Brazilian J. Vet. Res. Anim. Sci. 39, 324–30.Google Scholar
Danell, B. (1987). Oestrus behaviour, ovarian morphology and cyclical variation in the follicular system and endocrine pattern in water buffalo heifers. Ph.D Dissertation, Swedish University of Agricultural Sciences, Uppsala, Sweden.Google Scholar
Eppig, J.J. (1991). Intercommunication between mammalian oocytes and companion somatic cells. Mol. Reprod. Dev. 13, 569–74.Google ScholarPubMed
Eppig, J.J. & Schroeder, A.C. (1989) Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilisation in vitro. Biol. Reprod. 41, 268–76.Google Scholar
Guoliang, X., Byskov, A.G. & Andersen, C.Y. (1994). Cumulus cells secrete a meiosis-inducing substance by stimulation with forskolin and dibutyric cyclic adenosine monophosphate. Mol. Reprod. Dev. 39, 17–24.CrossRefGoogle Scholar
Gupta, P.S.P., Nandi, S., Ravindranatha, B.M & Sarma, P.V. (2001a). Isolation of preantral follicles from buffalo ovaries. Vet. Rec. 148, 543–4.Google Scholar
Gupta, P.S.P., Nandi, S., Ravindranatha, B.M. & Sarma, P.V. (2001b). Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro. Reprod. Fertil. Dev. 13, 355–60.Google Scholar
Gupta, P.S.P., Nandi, S., Ravindranatha, B.M. & Sarma, P.V. (2002a). In vitro culture of buffalo (Bubalus bubalis) preantral follicles. Theriogenology 57, 1839–54.Google Scholar
Gupta, P.S.P., Nandi, S., Ravindranatha, B.M. & Sarma, P.V. (2002b). Trypan blue staining to differentiate live and dead buffalo oocytes and its effect on embryo development in vitro. Buffalo J. 18, 321–30.Google Scholar
Gupta, P.S.P., Ramesh, H.S., Nandi, S. & Ravindra, J.P. (2006) Recovery of large preantral follicles from buffalo ovary: effect of season and corpus luteum. Anim. Reprod. Sci. (in press). doi: 10.1016/j.anireprosci.2006.11.011Google Scholar
Gutierrez, C.G., Ralph, J.H., Telfer, E.E., Wilmut, I. & Webb, R. (2000). Growth and antrum formation of bovine preantral follicles in long term culture in vitro. Biol. Reprod. 62, 1322–28.Google Scholar
Huanmin, Z. & Yong, Z. (2000). In vitro development of caprine ovarian preantral follicles. Theriogenology 54, 641–50.Google Scholar
Itoh, T., Kacchi, M., Abe, H., Sendai, Y. & Hoshi, H. (2002). Growth, antrum formation and estradiol production of bovine preantral follicles cultured in a serum free medium. Biol. Reprod. 67, 1099–105.Google Scholar
Kezele, P., Nilsson, E.E. & Skinner, M.K. (2005). Keratinocyte growth factor acts as a mesenchymal factor that promotes ovarian primordial to primary follicle transition. Biol. Reprod. 73, 967–73.Google Scholar
Leong, I.P. & Shui, G. (2002). An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76, 69–75.Google Scholar
Li, R., Philips, D.M. & Mather, J.P. (1995). Activin promotes ovarian follicle development in vitro. Endocrinology 136, 849–56.Google Scholar
Martins, F.S., Santos, R.R., Celestino, J.J.H., Matos, M.H.T., Silva, G A., Ferreira, F.V.A. & Figueiredo, J.R. (2004). Development of goat preantral follicles in coconut water solution. Acta Scientiae Veterinariae 32 (Supplement), 136.Google Scholar
Nandi, S., Ravindranatha, B.M., Gupta, P.S.P. & Sarma, P.V. (2001). Effect of somatic cells monolayer on maturation of buffalo oocytes in vitro. Indian J. Anim. Sci. 71, 936–7.Google Scholar
Nandi, S., Raghu, H.M., Ravindranatha, B.M., Gupta, P.S.P. & Sarma, P.V. (2002). Effect of somatic cell co-culture and synthetic oviductal fluid on development of buffalo embryos in vitro. Online J. Physiol. 1, 26–32.Google Scholar
Nilsson, E.E., Doraiswamy, V. & Skinner, M.K. (2003). Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development. Mol. Reprod. Dev. 66, 237–46.Google Scholar
Parrot, J.A. & Skinner, M.K. (1997). Direct actions of KL on theca cell growth and differentiation during follicle development. Endocrinology 138, 3819–27.CrossRefGoogle Scholar
Prochazka, R., Naggova, E., Brem, G., Schellander, K. & Motlik, J. (1998). Secretion of cumulus expansion enabling factor (CEEF) in porcine follicles. Mol. Reprod. Dev. 49, 141–9.Google Scholar
Santos, S.S., Biondi, F.C., Corderio, M.S., Miranda, M.S., Dantas, J.K., Figuerido, J.R. & Ohashi, O.M. (2006). Isolation, follicular density, and culture of preantral follicles of buffalo fetuses of different ages. Anim. Reprod. Sci. 95, 1–15.Google Scholar
Silva, J.R.V., Lucci, C.M., Carvalho, F.C.A., Bao, S.N., Costa, S.H.F., Santos, R.R & Figueiredo, J.R. (2000). Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro. Theriogenology 54, 809–22.CrossRefGoogle ScholarPubMed
Silva, J.R.V., Van Den Hurk, R., Costa, S.H.F., Andrade, E.R., Nunes, A.P.A., Ferreira, F.V.A., Lobo, R.N.B., & Figueiredo, J.R. (2004). Survival and growth of primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. Anim. Reprod. Sci. 81, 273–86.Google Scholar
Songsasen, N. & Apimeteetumrong, M. (2002). Effects of beta-mercaptoethanol on formation of pronuclei and developmental competence of swamp buffalo oocytes. Anim. Reprod. Sci. 71, 193–202.Google Scholar
Spears, N., Boland, N.I., Murray, A.A. & Gosden, R.G. (1994). Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Hum. Reprod. 9, 527–32.Google Scholar
Vanderstichele, H., Delaey, B., De Winter, J., De Jong, F., Rombauts, I., Verhoeven, G., Dello, C., Van de Voorde, A. & Briers, T. (1994). Secretion of steroids, growth factors, and cytokines by immortalized mouse granulosa cell lines Biol. Reprod. 50, 1190–202.CrossRefGoogle Scholar
Vigne, J.L., Lisa, I., Halburnt, & Skinner, M.K. (1994). Characterization of bovine ovarian surface epithelium and stromal cells: identification of secreted proteins. Biol. Reprod. 51, 1213–21.Google Scholar
Wu, J., Carrel, D.T. & Wilcox, A.L. (2001a). Development of in vitro-matured oocytes from porcine preantral follicles following intracytoplasmic sperm injection. Biol. Reprod. 65, 1579–85.Google Scholar
Wu, J., Emery, B.R. & Carrel, D.T. (2001b). In vitro growth, maturation, fertilization and embryonic development of oocytes from porcine preantral follicles. Biol. Reprod. 64, 375–81.Google Scholar
Wu, M.F., Huang, W.T., Tsay, C., Hsu, H.F., Liu, B.T., Chiou, C.M., Yen, S.C., Cheng, S.P. & Ju, J.C. (2002). The stage-dependent inhibitory effect of porcine follicular cells on the development of preantral follicles. Anim. Reprod. Sci. 73, 73–88.Google Scholar