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Production of mouse by inter-strain inner cell mass replacement

  • Yue-Liang Zheng (a1) (a2), Man-Xi Jiang (a1), Ying-Chun OuYang (a1), Qing-Yuan Sun (a1) and Da-Yuan Chen (a1)...

The developmental ability of reconstructed blastocysts from C57BL/6 strain mouse inner cell masses (ICMs) and Kunming strain mouse trophoblasts was assessed. The procedure of ICM replacement was as follows: C57BL/6 ICMs were separated from the blastocysts using immunosurgery. A slit was made in the zona pellucida of a Kunming blastocyst to allow its ICM to extrude. The C57BL/6 ICM was injected into the Kunming blastocoele, and the extruded Kunming ICM was cut off. The reconstructed blastocysts were able to re-expand (77%) and hatch (27.3%) in vitro. A total of 64 reconstructed blastocysts and 124 Kunming blastocysts were co-transferred into the uteri of 11 pseudopregnant Kunming mice, and an ICM replacement offspring was born. The results indicate that reconstructed embryos obtained by inter-strain ICM replacement have the ability to develop to term. This technique may provide a method to solve the pregnancy failure in interspecific cloning.

Corresponding author
All correspondence to: D.-Y. Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. Tel: 8610 62560528. Fax: 8610 62565689. e-mail:
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  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
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