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Seminal cell-free DNA levels measured by PicoGreen fluorochrome are associated with sperm fertility criteria

Published online by Cambridge University Press:  07 March 2017

F. Costa ,
F. Barbisan ,
C.E. Assmann ,
N.K.F. Araújo ,
A.R. de Oliveira ,
J.P. Signori ,
F. Rogalski ,
B. Bonadiman ,
M. S. Fernandes  and
I.B.M. da Cruz
F. Costa
Affiliation:
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
F. Barbisan
Affiliation:
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
C.E. Assmann
Affiliation:
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
N.K.F. Araújo
Affiliation:
Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
A.R. de Oliveira
Affiliation:
Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
J.P. Signori
Affiliation:
Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
F. Rogalski
Affiliation:
Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
B. Bonadiman
Affiliation:
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
M. S. Fernandes
Affiliation:
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
I.B.M. da Cruz*
Affiliation:
Laboratório Biogenômica. Universidade Federal de Santa Maria.Av Roraima 1000, Prédio 19, Santa Maria-RS 97105900, Brazil Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria-RS, Brazil
*
All correspondence to: Ivana Beatrice Mânica da Cruz. Laboratório Biogenômica. Universidade Federal de Santa Maria. Av Roraima 1000, Prédio 19, Santa Maria-RS 97105900, Brazil. E-mail: ivana.ufsm@gmail.com
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Summary

Previous investigations suggested that elevated cell-free DNA (cfDNA) can indicate non-healthy states. However, the potential association between cfDNA seminal plasma levels and fertility sperm parameters has not yet been determined. Therefore, the present study evaluated the association between seminal cfDNA levels and sperm fertility criteria to determine the use of seminal cfDNA quantification. An in vivo protocol quantified cfDNA levels of semen samples obtained from 163 male patients using fluorescent PicoGreen dye staining. To confirm if semen cfDNA quantification is realistic, an in vitro complementary test was performed using three or four semen samples. The fresh sperm samples were exposed to paraquat that generates high levels of superoxide anion causing oxidative stress and cell mortality. The results showed significant association between dsDNA levels and several sperm fertility parameters, such as low viability and alterations of motility and morphology. The in vitro analysis confirmed the association between dsDNA levels and sperm viability. Together, these results suggest that dsDNA levels could be an important biomarker to test sperm fertility.

Keywords

Cell-freeDNAFertilitySperm
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 111 - 119
Copyright
Copyright © Cambridge University Press 2017 

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