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Spastin interacts with CRMP5 to promote spindle organization in mouse oocytes by severing microtubules

Published online by Cambridge University Press:  26 May 2021

Zhen Jin ,
Hua-Feng Shou ,
Jin-Wei Liu ,
Shan-Shan Jiang ,
Yan Shen ,
Wei-Ye Cheng  and
Lei-Lei Gao Open the ORCID record for Lei-Lei Gao [Opens in a new window]
Zhen Jin
Affiliation:
Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Hua-Feng Shou
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Jin-Wei Liu
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Shan-Shan Jiang
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Yan Shen
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Wei-Ye Cheng
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
Lei-Lei Gao*
Affiliation:
Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China
*
Author for correspondence: Lei-Lei Gao. Department of Gynaecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China. Email: gaoleilei198802@126.com
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Abstract

Microtubule-severing protein (MTSP) is critical for the survival of both mitotic and postmitotic cells. However, the study of MTSP during meiosis of mammalian oocytes has not been reported. We found that spastin, a member of the MTSP family, was highly expressed in oocytes and aggregated in spindle microtubules. After knocking down spastin by specific siRNA, the spindle microtubule density of meiotic oocytes decreased significantly. When the oocytes were cultured in vitro, the oocytes lacking spastin showed an obvious maturation disorder. Considering the microtubule-severing activity of spastin, we speculate that spastin on spindles may increase the number of microtubule broken ends by severing the microtubules, therefore playing a nucleating role, promoting spindle assembly and ensuring normal meiosis. In addition, we found the colocalization and interaction of collapsin response mediator protein 5 (CRMP5) and spastin in oocytes. CRMP5 can provide structural support and promote microtubule aggregation, creating transportation routes, and can interact with spastin in the microtubule activity of nerve cells (30). Knocking down CRMP5 may lead to spindle abnormalities and developmental disorders in oocytes. Overexpression of spastin may reverse the abnormal phenotype caused by the deletion of CRMP5. In summary, our data support a model in which the interaction between spastin and CRMP5 promotes the assembly of spindle microtubules in oocytes by controlling microtubule dynamics, therefore ensuring normal meiosis.

Keywords

CRMP5MeiosisMicrotubule-severing proteinSpastinSpindle
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 80 - 91
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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