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Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™

Published online by Cambridge University Press:  17 September 2018

Rosilane Gomes de Souza de Oliveira ,
Arley Faria José de Oliveira  and
Marle Angélica Villacorta Correa
Rosilane Gomes de Souza de Oliveira*
Affiliation:
Universidade Nilton Lins/Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-graduação em Aquicultura
Arley Faria José de Oliveira
Affiliation:
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000. CEP: 69077-000 – Manaus, Amazonas, Brazil
Marle Angélica Villacorta Correa
Affiliation:
Universidade Federal do Amazonas, Faculdade de Ciências Agrárias, Departamento de Ciências Pesqueiras, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000. CEP: 69077-000 – Manaus, Amazonas, Brazil
*
Author for correspondence: Rosilane Gomes de Souza de Oliveira – Universidade Nilton Lins, Av. Nilton Lins, 3259, Manaus-AM, CEP: 69058-030 – Manaus, Amazonas, Brazil. Tel: +55 92–36432022. Fax: +55 92–36432022. E-mail: rosilanegso@gmail.com
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Summary

The aim of this study was to evaluate if hormonal treatment causes changes in the morphology of matrinxã Brycon amazonicus spermatozoa. Twelve males were randomized into three treatment groups: 1, Ovaprim™ (0.5 ml/kg); 2, carp pituitary extract (CPE; 2.0 mg/kg); and 3, NaCl solution 0.9% – control group (0.5 ml/kg); with four replicates. Morphological sperm analysis was performed using an optical and scanning electron microscope. The percentage of normal spermatozoa (49.6±4.6% to 60.8±2.8%), with primary (26.1±6.4% to 45.3±4.5%) and secondary abnormalities (4.4±2.9% to 13.9±3.5%) did not differ significantly between treatment groups. There were no significant differences between treatments in relation to the primary abnormalities found in the head (10.5±3.8% to 25.5±6.3%), the midpiece (0.1±0.1% to 0.2±0.2%) and in the flagellum (18.9±3.8% to 15.5±3.1%), as well as in the secondary abnormalities, located in the head (0.8±0.4% to 7.8±4.6%) and in the flagellum (2.2±1.1% to 6.1±1.5%). When each abnormality was evaluated individually, only the percentage of degenerated head was higher in the CPE-induced group (24.9±5.9%) than the control group (7.2±3.1%). We concluded that the use of pituitary extract of carp or Ovaprim™ under the conditions of this experiment does not influence the percentage of sperm abnormalities in B. amazonicus.

Keywords

FishInducing hormonesMicroscopeMorphological changesSpermatozoa
Type
Research Article
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 279 - 285
Copyright
© Cambridge University Press 2018 

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