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Survivin is a critical regulator of spindle organization and chromosome segregation during rat oocyte meiotic maturation

Published online by Cambridge University Press:  04 October 2010

Ke Wang ,
Guang-Jian Jiang ,
Liang Wei ,
Xing-Wei Liang ,
De-Qiang Miao ,
Shao-Chen Sun ,
Lei Guo ,
Zhen-Bo Wang  and
Sheng-Sheng Lu
Ke Wang
Affiliation:
Department of Biochemistry and Molecular Biology, North China Coal Medical University, Tangshan 063000, China.
Guang-Jian Jiang*
Affiliation:
Department of Biochemistry and Molecular Biology, North China Coal Medical University, Tangshan 063000, China. Department of Biochemistry and Molecular Biology, North China Coal Medical University, Tangshan 063000, China.
Liang Wei
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Xing-Wei Liang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
De-Qiang Miao
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Shao-Chen Sun
Affiliation:
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, China.
Lei Guo
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Zhen-Bo Wang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Sheng-Sheng Lu
Affiliation:
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, China.
*
All correspondence to: Guang-Jian Jiang, Department of Biochemistry and Molecular Biology, North China Coal Medical University, Tangshan 063000, China. e-mail: guangjianjiang@sina.com
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Summary

Survivin is a novel member of the inhibitor of apoptosis gene family that bear baculoviral IAP repeats (BIRs), whose physiological roles in regulating meiotic cell cycle need to be determined. Confocal microscopy was employed to observe the localization of survivin in rat oocytes. At the germinal vesicle (GV) stage, survivin was mainly concentrated in the GV. At the prometaphase I (pro-MI) and metaphase I (MI) stage, survivin was mainly localized at the kinetochores, with a light staining detected on the chromosomes. After transition to anaphase I or telophase I stage, survivin migrated to the midbody, and signals on the kinetochores and chromosomes disappeared. At metaphase II (MII) stage, survivin became mainly localized at the kinetochores again. Microinjection of oocytes with anti-survivin antibodies at the beginning of the meiosis, thus blocking the normal function of survivin, resulted in abnormal spindle assembly, chromosome segregation and first polar body emission. These results suggest that survivin is involved in regulating the meiotic cell cycle in rat oocytes.

Keywords

MeiosisMicrotubulesOocyteSpindleSurvivin
Type
Research Article
Information
Zygote , Volume 19 , Issue 4 , November 2011 , pp. 307 - 313
Copyright
Copyright © Cambridge University Press 2010

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