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Transcription and DNA replication of sperm nuclei introduced into blastomeres of 2-cell mouse embryos

Published online by Cambridge University Press:  26 September 2008

Berenika Plusa ,
Maria A. Ciemerych ,
Ewa Borsuk  and
Andrzej K. Tarkowski
Berenika Plusa
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
Ewa Borsuk
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
Andrzej K. Tarkowski
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
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Summary

The aim of this study was to investigate the behaviour of sperm nuclei in the cytoplasm of the 2-cell mouse embryo. To this end, we produced hybrids between anucleate fertilised oocyte fragments and blastomeres of the 2-cell embryos. When sperm nuclei at the stage of decondensation or recondensation were introduced into blastomeres the development of male pronuclei was usually retarded and they never reached the size of the blastomere nuclei. These abortive male pronuclei were unable to initiate transcription but they were capable of synthesising DNA. The majority of sperm nuclei introduced into blastomeres as early male pronuclei developed normally and reached the size of the blastomere nuclei. They synthesised DNA simultaneously with blastomere nuclei and were transcriptionally active. In addition they participated in the cleavage division of hybrid cells. This shows that the very early male pronucleus when transmitted from the oocyte cytoplasm to the blastomere cytoplasm can respond positively to the new cytoplasmic factors, i.e. it undertakes both DNA replication and transcription according to the time schedule characteristic of the second cell cycle.

Keywords

BlastomereMouseReplicationSperm nucleusTranscription
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 289 - 299
Copyright
Copyright © Cambridge University Press 1997

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