The species diversity of snails and other hosts for trematodes in Iceland

As mentioned above, the diversity of free-living animals acting as potential hosts for trematodes is much lower in Iceland than in mainland Europe, and most of the groups seem to have colonised Iceland during the Holocene (Cortés-Guzmán et al. Reference Cortés-Guzmán, Sinclair, Hof, Kalusche and Haase2024; Panagiotakopulu Reference Panagiotakopulu2014). There are fewer species of all respective groups, and members of some classes are missing completely (i.e., amphibians and reptiles) (Biological Diversity in Iceland 2001).

In the trematode life cycles, molluscs, particularly snails (Gastropoda), are considered the most important group because with some exceptions, they are always used as the first intermediate hosts (Cribb et al. Reference Cribb, Bray, Olson and Littlewood2003). In Iceland, few species of aquatic snails were reported by Mandahl-Barth (Reference Mandahl-Barth1938) and Einarsson (Reference Einarsson1977), and summarised by, e.g. Glöer (Reference Glöer2019). Of lymnaeid snails, Ampullaceana balthica (L.) (originally Radix balthica (L.)) and Galba truncatula (O. F. Müller) were recorded, and from planorbid snails, Anisus spirorbis (L.), Gyraulus laevis (Alder), G. parvus (Say) and Bathyomphalus contortus (L.) were found in few localities (Glöer Reference Glöer2019; own observations). In a pond at Nordic House, Vatnsmýri in Reykjavík, Physa acuta Draparnaud was found (Skírnisson and Schleich, unpublished, Pantoja et al. Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021). Of bivalves, Pisidium spp. are common in Iceland, and so far, 11 species were recorded (Hallgrímsson Reference Hallgrímsson1990; Kuiper et al. Reference Kuiper, Økland, Knudsen, Koli, von Proschwitz and Valovirta1989).

Ampullaceana balthica is the most common and widespread snail in Iceland, occurring in all types of water bodies and tolerating elevated temperature in hot springs (Bolotov et al. Reference Bolotov, Aksenova, Bespalaya, Gofarov, Kondakov, Paltser, Stefansson, Travina and Vinarski2017). Phylogeographic analyses by Bolotov et al. (Reference Bolotov, Aksenova, Bespalaya, Gofarov, Kondakov, Paltser, Stefansson, Travina and Vinarski2017) suggest that A. balthica colonised Iceland after the last glaciation rather than surviving in glacial refugia. Identification of A. balthica largely benefited from molecular genetic methods, as this snail species is variable in its shell characters and has been frequently confused with related species in mainland Europe (Ampullaceana lagotis Schrank, Peregriana labiata (Rossmässler), P. peregra (O. F. Müller)) and it was previously reported under different names, such as Radix (Lymnaea) peregra (O. F. Müller) or R. ovata (Draparnaud) (Huňová et al. Reference Huňová, Kašný, Hampl, Leontovyč, Kuběna, Mikeš and Horák2012; Schniebs et al. Reference Schniebs, Glöer, Vinarski and Hundsdoerfer2011). Precise identification of the snail hosts has a substantial practical impact on parasitology, as different snail species might vary in susceptibility (Huňová et al. Reference Huňová, Kašný, Hampl, Leontovyč, Kuběna, Mikeš and Horák2012).

Gyraulus parvus is widely distributed in North America. It is present up to south Greenland and is invasive in Europe (Glöer Reference Glöer2019). However, Lorencová et al. (Reference Lorencová, Beran, Nováková, Horsáková, Rowson, Hlaváč, Nekola and Horsák2021) in their phylogenetic analyses based on DNA sequence data have revealed that G. parvus and G. laevis, the latter considered native in Europe, are in fact part of the same species-level clade and should be recognised as G. parvus. The Icelandic population of Gyraulus (or race; for details, see Lorencová et al. Reference Lorencová, Beran, Nováková, Horsáková, Rowson, Hlaváč, Nekola and Horsák2021) belongs to the one originally assumed to be G. laevis. This would imply that G. parvus expanded to Iceland via mainland Europe, indicating a possibly recent time of colonisation in the early to mid-Holocene (Lorencová et al. Reference Lorencová, Beran, Nováková, Horsáková, Rowson, Hlaváč, Nekola and Horsák2021). This might have further implications for the trematode species spectrum, as one of the trematode parasites (Neopetasiger islandicus) found in G. parvus in Iceland was recorded in several bird species in North America (Tkach et al. Reference Tkach, Kudlai and Kostadinova2016).

Physa acuta is of American origin, and it is highly invasive. It can efficiently disperse via water, aquatic birds, and mammals, and it is present in almost all continents (Lorencová et al. Reference Lorencová, Beran, Nováková, Horsáková, Rowson, Hlaváč, Nekola and Horsák2021; van Leeuwen et al. Reference van Leeuwen, Huig, van der Velde, van Alen, Wagemaker, Sherman, Klaassen and Figuerola2013). It was described from France as a new species in 1805, and it further spread to Great Britain probably in the second half of the 19th century, either via human activity or bird transport (Anderson Reference Anderson2003; Vinarski Reference Vinarski2017). Physa acuta was introduced to Iceland more than 40 years ago by human activity, and since then, there is a stable population in Nordic House, Vatnsmýri in Reykjavík (Skírnisson and Schleich, unpublished).

There are five native fish species in Iceland which live wholly or partly in freshwater: Atlantic salmon (Salmo salar L.), which is known to enter Icelandic rivers and lakes, Arctic charr (Salvelinus alpinus (L.)), brown trout (Salmo trutta L.), European eel (Anguilla anguilla (L.)), and three-spined stickleback (Gasterosteus aculeatus L.) (Biological Diversity in Iceland 2001). There are two non-native species: rainbow trout (Oncorhynchus mykiss (Walbaum)) which was introduced to Iceland with fish farming and escaped to the wild, and the Pacific pink salmon (Oncorhynchus gorbuscha (Walbaum)), which has been spreading since 1960 (Biological Diversity in Iceland 2001; Finnsson et al. Reference Finnsson, Chambers and Guðbergsson2025). Most of the fish species occur all over Iceland in rivers and lakes; only European eel is more common in the western and southwestern part of the island. The distribution of fishes in northern latitudes has been substantially influenced by glaciation events in the Pleistocene, and the current view is that fishes have rapidly recolonised Iceland after the last glaciation – for example, Arctic charr most probably diverging from one North Atlantic lineage (Bernatchez and Wilson Reference Bernatchez and Wilson1998; Brunner et al. Reference Brunner, Douglas, Osinov, Wilson and Bernatchez2001; McKeown et al. Reference McKeown, Hynes, Duguid, Ferguson and Prodöhl2010; Wilson et al. Reference Wilson, Gíslason, Skúlason, Snorasson, Adams, Alexander, Danzmann and Ferguson2004). Currently, the native Arctic charr, brown trout, and Atlantic salmon are affected by climate warming, while the cold-adapted Arctic charr is the most vulnerable, and its populations have declined within the last 30 years (Jeppessen et al. 2010; Svenning et al. Reference Svenning, Falkegård, Dempson, Power, Bårdsen, Guðbergsson and Fauchald2022).

Birds comprise the greatest faunal diversity in Iceland, with 88 species nesting regularly and about 424 species being recorded in total (Einarsson Reference Einarsson, Heath and Evans2000; Lepage et al. Reference Lepage, Vaidya and Guralnick2014). They are assumed to be predominantly of European origin, breeding around the northern hemisphere or particularly in the North Atlantic region (Lepage et al. Reference Lepage, Vaidya and Guralnick2014). In Iceland, the most abundant are waterfowl, seabirds, and waders (Einarsson Reference Einarsson, Heath and Evans2000). One of the most important nesting sites is Lake Mývatn (a designated Ramsar site), with all the waterfowl species known from Iceland breeding there (Biological Diversity in Iceland 2001; Einarsson Reference Einarsson, Heath and Evans2000). Iceland is not only a major breeding region, but it is also an important stopover for migratory birds nesting in the Arctic (Canada, Greenland) and wintering further south (Einarsson Reference Einarsson, Heath and Evans2000).

The mammalian fauna living in the wild in Iceland is considered to consist of four species. The Arctic fox (Vulpes lagopus (L.)) is the only native mammal species in Iceland, living in inland and coastal areas (Hersteinsson and MacDonald Reference Hersteinsson and MacDonald1996). The Arctic fox colonised Iceland during the Little Ice Age, before humans arrived (Mellows et al. Reference Mellows, Barnett, Dalén, Sandoval-Castellanos, Linderholm, McGovern, Church and Larson2012). All other mammal species were introduced by humans – the wood mouse (Apodemus sylvaticus (L.)), reindeer (Rangifer tarandus (L.)), and mink (Neogale vison (Schreber)); completely bound to human settlements are Mus musculus L., Rattus rattus (L.), and R. norvegicus (Berkenhout) (Biological Diversity in Iceland 2001).

Icelandic literature collection and parasite-host records

To compile a comprehensive overview, we searched for published literature, locally published sources, and master/doctoral theses via Scopus, Science Direct, and Google Scholar. The key words we used for the search were ‘Iceland AND Trematoda OR Digenea’. Further, we searched our personal library collections, particularly that of K. Skírnisson in Iceland.

We compiled a database of species-level distribution data consisting of a total of 48 relevant papers, published between 1956 and 2024, including articles published in Icelandic, conference abstracts, and two theses. The database comprises host-parasite locality records for trematode parasites of snails (three species), fishes (four species), birds (12 species), and mammals (one species) in freshwater habitats in Iceland. The database contains 222 records of 41 trematode species of 19 genera and 9 families (Table 1). The most frequently recorded families were Schistosomatidae, Diplostomidae, Strigeidae, Allocreadiidae, and Echinostomatidae. The most recorded species were Diplostomum spathaceum, Crepidostomum farionis, and Trichobilharzia franki “peregra”. The snail species Ampullaceana balthica was recorded as the most commonly used first intermediate host by 21 trematode species of six families. Of the fishes, four species were found to be hosts for 13 trematode species of five families. The majority of fish records were as second intermediate hosts infected by metacercariae of Diplostomum and Apatemon. A total of 16 trematode species of six families were recorded in 12 species of birds (definitive hosts). Out of the 41 trematode species recorded, for five species (Neopetasiger islandicus, Trichobilharzia anseri, T. franki “peregra”, T. mergi, and T. regenti), the whole life cycle was documented in Iceland. For Apatemon gracilis and Diplostomum spp., larval stages from both snails (cercariae) and fishes (metacercariae) were documented. For Cotylurus cornutus, Echinoparyphium recurvatum and Echinostoma revolutum, both larval stages and adults, were recorded; however, those were not linked using DNA sequence data.

Table 1.

List of freshwater trematode species recorded in Iceland between 1956 and 2024 (order of families follows that of Olson et al. 2003)

* abbreviations for hosts: 1. – first intermediate host; 2. – second intermediate host; D – definitive host

^** none of the records were identified to species level, and we assume there were more species involved

^† empty shells with adolescariae (metacercariae encysted in the environment) were used for experimental obtaining of adults

^†† Fulica atra does not breed in Iceland; via drift migration, non-indigenous birds enter Iceland and might carry parasites with no connections to Iceland

Of the seven freshwater gastropod species known from Iceland, three species were found infected with trematodes: Ampullaceana balthica, Gyraulus parvus, and Physa acuta. Galba truncatula, an intermediate host for Fasciola hepatica Linnaeus, 1758, was recorded only at a few sites and was never found infected. Ampullaceana balthica was found as the first intermediate host of 21 trematode species, which by far exceeds all other snail species recorded and is comparable to Lymnaea stagnalis (L.) hosting 24 species in central Europe (Faltýnková et al. Reference Faltýnková, Našincová and Kablásková2007). An explanation of this phenomenon might lie in the fact that A. balthica is one of the most widespread radicine snail species in mainland Europe, its distributional range reaching from north to south and extending eastwards to Siberia and Central Asia (Aksenova et al. Reference Aksenova, Bolotov, Gofarov, Kondalov, Vinarski, Bespalaya, Kolosova, Palatov, Sokolova, Spitsyn, Tomilova, Travina and Vikhrev2018; Bolotov et al. Reference Bolotov, Aksenova, Bespalaya, Gofarov, Kondakov, Paltser, Stefansson, Travina and Vinarski2017; Schniebs et al. Reference Schniebs, Glöer, Vinarski and Hundsdoerfer2011; Vinarski et al. Reference Vinarski, Bolotov, Aksenova, Babushkin, Bespalaya, Makhrov, Nekhaev and Vikhrev2021). Since it has been found to host a wide variety of trematode species in Europe (Kundid et al. Reference Kundid, Pantoja, Janovcová and Soldánová2024), we can infer that it is highly probable that trematodes using A. balthica in mainland Europe are able to complete their life cycles in Iceland as well, when they can find their susceptible snail host.

Of the planorbid snails, G. parvus was found infected with two trematode species, N. islandicus and Apatemon sp. 6. It is peculiar that both species possess cercariae with a large tail, mimicking prey to attract fish hosts (Faltýnková et al. Reference Faltýnková, Kudlai, Pantoja, Jouet and Skírnisson2023).

In P. acuta, one single species, Echinoparyphium sp. 2, was recorded by Pantoja et al. (Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021), which is conspecific with material of Gordy and Hanington (Reference Gordy and Hanington2019) from Canada. The American origin of P. acuta might explain the presence of this American trematode species. There is a stable population of P. acuta in Nordic House, Vatnsmýri in Reykjavík, where the first echinostome and strigeid metacercariae were found in 2007 (Skírnisson and Schleich, unpublished), and cercariae of Echinoparyphium sp. 2 were recorded 14 years later by Pantoja et al. (Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021).

Pisidium spp. so far have not been found infected, although they are known as intermediate hosts of allocreadiids and gorgoderids from, e.g. mainland Europe (Kristmundsson and Richter Reference Kristmundsson and Richter2009).

Fish play an important role particularly as second intermediate hosts in trematode life cycles in Iceland. A total of 73% of the fish trematode records concern metacercariae of Diplostomum spp. or Apatemon spp., comprising 11 trematode species. Of trematodes using fishes as definitive hosts, four species were found from two genera (Crepidostomum and Phyllodistomum) in Salmo trutta and Salvelinus alpinus. Anguilla anguilla and Gasterosteus aculeatus were found to host only metacercariae. The highest number of trematode species was recorded in G. aculeatus (i.e., nine species (see Table 1)), which indicates that this fish species plays an important role in transmission of Diplostomum and Apatemon in Iceland and probably in other sub-Arctic areas (Blasco-Costa et al. Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014). In S. trutta, five trematode species were recorded, and in S. alpinus, four. The populations of the mentioned salmonids can be truly landlocked, resident, or anadromous (Brunner et al. Reference Brunner, Douglas, Osinov, Wilson and Bernatchez2001), and it is likely that migration of the fish might be responsible for spreading of the parasites (i.e., Crepidostomum and Phyllodistomum). Also, in Hafravatn, it was recorded that Salmo salar is entering this lake (Kristmundsson and Richter Reference Kristmundsson and Richter2009), which might further contribute to dispersion of trematodes between Iceland and mainland Europe.

Although birds are the group of organisms probably most contributing to the trematode diversity in Iceland, there are fewest records of them as definitive hosts. This can be due to ethical and practical reasons, as it is more difficult to obtain birds for dissections. In Iceland, birds of the Anatidae, Podicipedidae, Laridae, and Rallidae were examined and were found to harbour 16 trematode species. Most records comprise bird schistosomes (Schistosomatidae) because they occur at high prevalences in Icelandic birds (Skírnisson and Kolářová Reference Skírnisson and Kolářová2008), and researchers focused on this group of trematodes, as they are of medical importance. Judging from the records, the trematode diversity in birds is understudied, and many trematode species found in snails (e.g., Diplostomum, Plagiorchis) surely could be found in birds as well.

Sampling locations in Iceland

We found records of 50 locations in Iceland from where material was obtained; they were predominantly lakes, of which most are of volcanic origin, or ponds; to a lesser extent, rivers or small brooks, and areas near the shore, were included (Figure 1). There was likely bias in the selection of sampling locations, as the central part of Iceland is largely covered with glaciers and is poorly accessible. Among the most sampled locations were those in Reykjavík (Nordic House, Vatnsmýri; Family Park in Laugardalur) and lakes in its proximity (Rauðavatn, Hafravatn, Elliðavatn). In SW Iceland, it was Lake Þingvallavatn, the largest lake in Iceland, from where fishes were obtained for ecological studies. In the North, the most sampled lake was Mývatn, which is eutrophic, with thermal vents and a high density of nesting birds (Einarsson Reference Einarsson2004). Some of the lakes are connected with the sea and can be entered by anadromous salmonid fishes or catadromous eels (Anguilla anguilla), which could be responsible for spreading parasites originating from areas outside Iceland (Faltýnková et al. Reference Faltýnková, Pantoja, Skírnisson and Kudlai2020).

Figure 1.

Localities in Iceland where sampling was done. Numbers in squares indicate how many trematode species were recovered; places with no findings are also included.

In search for causative agents of cercarial dermatitis (bird schistosomes) affecting people bathing in water, a large number of localities were visited to collect snails or birds, the most famous being Landmannalaugar and Botnsvatn (Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009). Those surveys extended the number of locations examined for trematodes; however, mostly only bird schistosomes were recorded there. Anyway, in most of the localities (i.e., 34), single trematode species were recorded. Lake Mývatn was identified as a hotspot for trematode diversity, with 13 species recorded. And a total of 10 species were found in each of Nordic House, Vatnsmýri in Reykjavík, and Hafravatn; the first site is a bird reserve, and both sites have been quite intensively sampled. Only a few sampling localities (7) had no trematodes recorded.

Historical accounts of trematode discovery in Iceland

The very first mention of a trematode from Iceland was that of Odhner (Reference Odhner1902, Reference Odhner1905), which was a marine record (Lepidophyllum steenstrupi Odhner, Reference Odhner1902, from the spotted wolffish, Anarhichas minor Olafsen). The first comprehensive parasite study focused on different host groups from Iceland was that of Brinkmann (Reference Brinkmann1956), who examined mammals (2 species), birds (16 spp.), and fishes (18 spp.) from Reykjavík, Neskaupstaður, Lake Mývatn, and Húsavík. Brinkmann (Reference Brinkmann1956) found 24 species of trematodes, of which most were new records for Iceland. Many of the recorded species were marine, and Brinkmann (Reference Brinkmann1956) assumed some to be introduced by migratory birds. Interestingly, Brinkmann (Reference Brinkmann1956) reported that no zoogeographically Arctic group of fishes could be distinguished, and consequently, no specifically Arctic trematodes were recovered. His conclusion was that the species were rather boreo-Arctic. The same was stated before by Odhner (Reference Odhner1905) for those from the marine environment; both studies revealed a wider distribution of trematodes in the northern regions than previously expected.

The first study on larval trematodes from freshwater snails and fishes in Iceland was conducted by Blair (Reference Blair1973), who, during the Glasgow expedition in 1972, examined Ampullaceana balthica (by that time reported as Lymnaea peregra), Galba truncatula, Oxyloma elegans groenlandica (Møller) (= Succinea groenlandica Møller) (terrestrial snail of the family Succineidae), and Gasterosteus aculeatus from south Iceland. Blair (Reference Blair1973) found a diverse spectrum of strigeid, echinostome, and notocotylid cercariae/metacercariae in A. balthica and diplostomid and strigeid metacercariae in G. aculeatus. The identity of some of those species was verified experimentally by completing their life cycles (see also Blair Reference Blair1976, Reference Blair1977). No digeneans were found in O. elegans groenlandica or in G. truncatula. Blair (Reference Blair1973) was the first who proved that trematode larval stages are present in intermediate hosts in Iceland and that trematode life cycles are likely to be completed there.

In the following years, the attention of Icelandic researchers was mainly focused on ecology of fish trematodes and their health impact on fish populations of Salvelinus alpinus, Salmo trutta, and G. aculeatus (Frey et al. Reference Frey, Barrett, Parkin, Blakeley, Ålund, Byford, Euhus, Tsarnas, Boughman and Stenkamp2022; Karvonen et al. Reference Karvonen, Kristjánsson, Skúlason, Lanki, Rellstab and Jokela2013; Kristmundsson and Richter Reference Kristmundsson and Richter2003, Reference Kristmundsson and Richter2009; Natsopoulou et al. Reference Natsopoulou, Pálsson and Ólafsdóttir2012; Richter Reference Richter1982, Reference Richter2003). Frandsen et al. (Reference Frandsen, Malmquist and Snorrason1989) examined the degree of parasitism in different fish morphs of S. alpinus in Þingvallavatn and found an ecological segregation affecting the parasite transmission pathways. Parasites of European eels were examined by Kristmundsson and Helgason (Reference Kristmundsson and Helgason2007), who discovered a species-poor freshwater parasite fauna compared to other parts of Europe, and Diplostomum spp. were the most prevalent helminth species.

Since the 1990s, most trematode research was directed on bird schistosomes (family Schistosomatidae), which cause cercarial dermatitis, an inflammatory reaction of the skin in humans (Horák et al. Reference Horák, Kolářová and Adema2002). A massive outbreak of this serious rash was recorded in a recreational area in Reykjavík from 1995 to 1997 (Skírnisson et al. Reference Skírnisson, Magnússon, Kristjánsdóttir and Kolářová1999, Skírnisson and Kolářová Reference Skírnisson and Kolářová2002, Reference Skírnisson, Aldhoun and Kolářová2009; Kolářová et al. Reference Kolářová, Skírnisson and Horák1999a). Since then, bird schistosomes have been the best documented group in Iceland in the attempt to identify their species and to study their life cycles, biology, and distribution.

With the advance of molecular genetic methods, since 2006 in Iceland, an unexpected diversity of trematode species was discovered and characterised both genetically and morphologically. This led to descriptions of new species and the discovery of a wealth of previously cryptic species with unexpected connections to other regions in Europe and North America. Mainly species of the groups Diplostomidae, Strigeidae, Schistosomatidae, Echinostomatidae, Plagiorchiidae, Allocreadiidae, and Gorgoderidae were recorded, which are detailed below.

Bird schistosomes and cercarial dermatitis (Schistosomatidae)

Bird schistosomes are cosmopolitan trematodes parasitising aquatic birds and using mostly pulmonate snails as intermediate hosts in a two-host life cycle (Horák et al. Reference Horák, Kolářová and Adema2002). In Iceland, the occurrence of cercarial dermatitis in humans caused by bird schistosomes can be traced back to 1925. At that time in Sýkið, a geothermally heated shallow pond in the vicinity of Deildartunguhver, and the largest thermal spring in Iceland, a skin rash was observed on wading children (see Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009). The first scientifically documented record of the causative agent of cercarial dermatitis, the cercaria, is that of Kolářová et al. (Reference Kolářová, Skírnisson and Horák1999a). The cercariae were obtained from snails (Ampullaceana balthica) in a recreation area in Reykjavík (pond in Family Park in Laugardalur), where in summer of 1995–1997, a skin rash was documented in children wading in the pond (Kolářová et al. Reference Kolářová, Skírnisson and Horák1999a, Reference Kolářová, Skírnisson, Horák and Sitkob; Skírnisson and Kolářová Reference Skírnisson and Kolářová2002). The authors described the cercariae morphologically (Cercaria sp.) and showed that they probably belong to the genus Trichobilharzia. However, they were not successful in obtaining adults experimentally, which at that time was the only way to identify the species; and no DNA sequences could be obtained.

Further cases of cercarial dermatitis were recorded in geothermally heated water bodies, which attract people for bathing, and where snails and birds necessary for completion of the life cycle were present. A severe outbreak of cercarial dermatitis was documented in Landmannalaugar (Skírnisson and Kolářová Reference Skírnisson and Kolářová2005; Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009), which is a famous geothermally heated bathing site. Infected A. balthica and mallards (Anas platyrhynchos L.) were recovered at that site even in winter months; after finding the causative agents, the presence of cercariae in snails could be eliminated by preventing the birds from breeding at that site (Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009). Another lake was Botnsvatn, from where two species of the genus Trichobilharzia were documented (see Table 1) and where one of the highest prevalences of bird schistosomes in A. balthica was found (Skírnisson and Kolářová Reference Skírnisson and Kolářová2008; Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009).

Since the first case in Family Park, several studies followed which aimed to identify the causative agents of cercarial dermatitis and to assess its prevalence, therefore, freshwater snails and aquatic birds have been screened intensively (see Kolářová and Skírnisson Reference Kolářová and Skírnisson2000; Kolářová et al. Reference Kolářová, Skírnisson, Horák and Sitko1999b; Kolářová et al. Reference Kolářová, Skírnisson, Rudolfová, Jouet, Léger and Ferté2005; Skírnisson and Kolářová Reference Skírnisson and Kolářová2002, Reference Skírnisson and Kolářová2004). The methodology used to collect and examine snails and aquatic birds has been summarised by Kolářová et al. (Reference Kolářová, Horák and Skírnisson2010). Waterfowl (A. platyrhynchos, Anser anser (L.) and Cygnus cygnus (L.)) were found infected with nasal and visceral species of bird schistosomes, whereas the examined specimens of Anas penelope (L.), Bucephala islandica (Gmelin), Mergus serrator L., and Somateria mollissima (L.) were free of infection (Kolářová et al. Reference Kolářová, Skírnisson, Rudolfová, Jouet, Léger and Ferté2005). In 2006, the first study focused on Icelandic samples was published using integrative taxonomic methods (phylogenetic analyses of DNA sequence data in combination with morphology), and a new species and genus of bird schistosomes, Allobilharzia visceralis, was described by Kolářová et al. (Reference Kolářová, Rudolfová, Hampl and Skírnisson2006) from whooper swans (C. cygnus). To identify the material of bird schistosomes found in 2002–2007 from A. balthica and anseriform birds, Aldhoun et al. (Reference Aldhoun, Kolářová and Horák2009) analysed the samples using molecular genetic methods (based on the entire 18S-ITS1-5.8S-ITS2-28S sequence) and could distinguish five species/lineages of two different genera, of which some were identical to species found in mainland Europe. Skírnisson and Kolářová (Reference Skírnisson and Kolářová2008) examined the morphology and dimensions of eggs of bird schistosomes and could distinguish seven species occurring in Iceland. A considerable number were classified provisionally, and only in recent times, some could be assigned to species via integrative analyses using various molecular markers in combination with morphology. Among them was the nasal species, Trichobilharzia regenti, which was proved as common in Iceland, and Jouet et al. (Reference Jouet, Skírnisson, Kolářová and Ferté2010a) and Skírnisson et al. (Reference Skírnisson, Kolářová, Horák, Ferté and Jouet2012) improved the knowledge of its morphological variation and host use by providing molecular and morphological data obtained from wildlife, as the species was originally only described from limited experimental material of adults in the Czech Republic (Horák et al. Reference Horák, Kolářová and Dvořák1998).

Jouet et al. (Reference Jouet, Skírnisson, Kolářová and Ferté2010b) attempted to clarify the status of T. franki and its haplotypes, which turned out to comprise two clades using two different snail species as intermediate hosts. The outcome was that T. franki using Radix auricularia (L.) is distributed in mainland Europe and corresponds to the original species described by Müller and Kiming (Reference Müller and Kimmig1994), whereas a different species (tentatively named T. franki “peregra” by Jouet et al. Reference Jouet, Skírnisson, Kolářová and Ferté2010b) using A. balthica is present in Europe and Iceland, and its status has yet to be clarified. Kolářová et al. (Reference Kolářová, Skírnisson, Ferté and Jouet2013) described a new species, T. mergi, from Mergus serrator from Botnsvatn corresponding to the material of small eggs (Trichobilharzia sp. V) isolated by Skírnisson and Kolářová (Reference Skírnisson and Kolářová2008) (Table 1); via DNA matching of cercariae, they revealed that the intermediate host was A. balthica. It was shown that the life cycle is being completed in Lake Botnsvatn, as both adults and cercariae originated from host samples collected at this lake. Since M. serrator is a migratory species, it is expected that T. mergi might have a Holarctic distribution; so far, it was reported from France and Belarus (Kolářová et al. Reference Kolářová, Skírnisson, Ferté and Jouet2013). Jouet et al. (Reference Jouet, Kolářová, Patrelle, Ferté and Skírnisson2015) described T. anseri from A. anser in Family Park, Laugardalur in Reykjavík; the species is identical to that first documented by Kolářová et al. (Reference Kolářová, Skírnisson and Horák1999a) as the causative agent of cercarial dermatitis, and to the material of eggs (Trichobilharzia sp. II) isolated from A. anser by Skírnisson and Kolářová (Reference Skírnisson and Kolářová2008). Trichobilharzia anseri was also recorded from France (Jouet et al. Reference Jouet, Kolářová, Patrelle, Ferté and Skírnisson2015) (see Figure 2).

Figure 2.

Selected trematode families and their distribution in Iceland and other countries. Only records confirmed by DNA sequence data are included.

There is a considerable species diversity of bird schistosomes documented in Iceland, despite the fact that only one snail species (A. balthica) was recorded as the first intermediate host (Aldhoun et al. Reference Aldhoun, Kolářová and Horák2009). So far, eight species of three genera (Allobilharzia, Dendritobilharzia, and Trichobilharzia) of bird schistosomes have been reported (see Table 1), with at least one species of Trichobilharzia still to be formally described and one species probably belonging to a new genus (Aldhoun et al. Reference Aldhoun, Kolářová and Horák2009; Skírnisson Reference Skírnisson2010). Anseriform birds serve as definitive hosts, including three species which overwinter in Iceland (A. platyrhynchos, A. anser, and M. serrator), and C. cygnus which leaves Iceland for wintering grounds on the British Isles or western Norway (Skírnisson and Kolářová Reference Skírnisson and Kolářová2008; Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009). This indicates that the life cycles of most of the bird schistosomes are being completed in Iceland, except for A. visceralis, which was only found in adult whooper swans after their arrival from wintering grounds outside Iceland (Skírnisson et al. Reference Skírnisson, Aldhoun and Kolářová2009). Meanwhile, A. visceralis has been reported from North America ex Cygnus columbianus (Ord) and from Japan ex C. cygnus (Brant Reference Brant2007; Hayashi et al. Reference Hayashi, Ichikawa-Seki, Ohari, Mohanta, Aita, Satoh, Ehara, Tokashiki, Shiroma, Azuta, Oka, Watanabe, Harasawa, Inohana, Ichijo and Furuhama2017). Swans have a Holarctic circumpolar distribution and could overlap in their breeding range, which might explain the geographical distribution of A. visceralis (Brant Reference Brant2007) (see Figure 2). However, its intermediate snail host is still unknown; Brant (Reference Brant2007) assumed that it could be a brackish or marine snail from habitats where swans feed.

Trematode fauna of fish (Allocreadiidae, Gorgoderidae)

There is a long tradition of fish monitoring in Iceland (Petersen Reference Petersen2003), and thus, their parasite fauna has been studied to assess their health status since fishes are of substantial importance in ecosystems and are vital natural resources. Records of adult trematodes from freshwater fishes in Iceland range from 1956 to 2020, and four species of the family Allocreadiidae and Gorgoderidae have been discovered in Salvelinus alpinus and Salmo trutta, whereas no adult trematodes were found in Gasterosteus aculeatus and Anguilla anguilla (see also Table 1). Brinkmann (Reference Brinkmann1956) recorded Crepidostomum farionis from S. alpinus and S. trutta and differentiated the species from C. suecicum Nybelin, 1933 (now a synonym of C. metoecus (Braun, 1900)), to which he considered the species most similar and provided a drawing but no thorough description. Richter (Reference Richter1982) briefly disclosed that adults of C. farionis and Phyllodistomum conostomum (Olsson, 1876) (a synonym of P. umblae) occur in Icelandic fishes. Further studies pursued ecological aspects of fish infection, but no taxonomic questions. Frandsen et al. (Reference Frandsen, Malmquist and Snorrason1989) examined the parasite burden in different ecological morphs of S. alpinus in Lake Þingvallavatn and found C. farionis in benthivorous fish morphs, which corresponds to the habitat of its intermediate hosts (clams, amphipods, ephemeropterans, which live in shallow littoral zones). Another brief review by Kristmundsson and Richter (Reference Kristmundsson and Richter2003) reported on the occurrence of Crepidostomum sp. and Phyllodistomum sp. in Arctic charr, however, with no specific identification. A detailed survey of the parasite fauna of the resident Arctic charr and brown trout was undertaken by Kristmundsson and Richter (Reference Kristmundsson and Richter2009) who reported C. farionis as a common parasite in both fish species, while P. umblae was more prevalent in S. alpinus; intermediate hosts for these species have not yet been documented in Iceland.

By the use of integrative taxonomy, Faltýnková et al. (Reference Faltýnková, Pantoja, Skírnisson and Kudlai2020) identified four species of trematodes in freshwater fishes in Iceland, the already known C. farionis and P. umblae, and two more species of Crepidostomum: C. brinkmanni and C. pseudofarionis (see also Table 1). The two latter species are morphologically highly similar to their genetically closest species (C. metoecus and C. oschmarini Zhokhov & Pugacheva, 1998; and C. farionis, respectively). Therefore, the use of integrative taxonomy is required in future studies, as species of both Crepidostomum and Phyllodistomum possess too few distinguishing morphological features and high plasticity of characters. Moreover, for correct assessment of the distributional range and host specificity of these species, more material processed by an integrative taxonomic approach from different geographical areas, including North America, is still needed.

Interestingly, prior to their formal species description, C. brinkmanni and C. pseudofarionis had their life cycles deciphered molecularly by Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017) and Petkevičiūtė et al. (Reference Petkevičiūtė, Stunžėnas, Zhokhov, Poddubnaya and Stanevičiūtė2018) in Norway. Both C. brinkmanni and C. pseudofarionis have a three-host life cycle involving clams of the genera Sphaerium and Pisidium as first intermediate hosts and mayflies (Ephemeroptera) and stoneflies (Plecoptera) as second intermediate hosts (Kristmundsson and Richter Reference Kristmundsson and Richter2009). The life cycle of P. umblae was molecularly characterised by Petkevičiūtė et al. (Reference Petkevičiūtė, Stunžėnas, Stanevičiūtė and Zhokhov2015) using material from Norway (cystocercous cercariae from Euglesa parvula (Westerlund) (=Pisidium hibernicum Westerlund) and Sphaerium corneum (L.); adults from Thymalus thymallus (L.) and Coregonus albula (L.)). From the common occurrence of Crepidostomum spp. and P. umblae in fishes in Iceland, we infer that their life cycles are completed there, as sphaeriid clams (Pisidium, first intermediate hosts) and mayflies, stoneflies, and crustaceans (second intermediate hosts) are present in Icelandic lakes, although their species diversity is much lower than in Europe (Kristmundsson and Richter Reference Kristmundsson and Richter2009; Lindegaard Reference Lindegaard1979).

It is confirmed now by molecular genetic methods that Crepidostomum spp. and P. umblae have been found outside Iceland; that is, C. brinkmanni, C. farionis, and C. pseudofarionis were recorded in Norway by Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017) and Petkevičiūtė et al. (Reference Petkevičiūtė, Stunžėnas, Zhokhov, Poddubnaya and Stanevičiūtė2018), and C. brinkmanni was found by Petkevičiūtė et al. (Reference Petkevičiūtė, Stunžėnas, Zhokhov, Poddubnaya and Stanevičiūtė2018) in Ukraine. Crepidostomum farionis was found in the Russian Far East by Atopkin and Shedko (Reference Atopkin and Shedko2014) (see Figure 2). The connection of Iceland with mainland Europe might be explained by the fact that Lake Hafravatn, from where the Icelandic material was recovered, is connected to the sea, and andromous Atlantic salmon, Salmo salar, which might carry infections of Crepidostomum or Phyllodistomum (Kennedy Reference Kennedy1974), enter the lake (Kristmundsson and Richter Reference Kristmundsson and Richter2009). Another way of dispersion could be via sphaeriid clams which have a high capacity for dispersion by migratory birds (Clewing et al. Reference Clewing, Bössneck, von Oheimb and Albrecht2013; Figuerola and Green Reference Figuerola and Green2002). However, further sampling from a wider geographical scale is necessary to explain the distributional patterns of species of both Crepidostomum and Phyllodistomum.

Diplostomidae

Before the start of the molecular era, in Iceland, trematodes of the family Diplostomidae have been recorded frequently as metacercariae from the eyes of fishes (Anguilla anguilla, Gasterosteus aculeatus, Salmo trutta, and Salvelinus alpinus). At that time, reliable species identification was difficult, as metacercariae exhibit too few features suitable for identification. Therefore, the recovered metacercariae were mostly recorded as Diplostomum spathaceum or Diplostomum sp. by Frandsen et al. (Reference Frandsen, Malmquist and Snorrason1989), Kristmundsson and Richter (Reference Kristmundsson and Richter2003, Reference Kristmundsson and Richter2009), and Richter (Reference Richter2003). Natsopoulou et al. (Reference Natsopoulou, Pálsson and Ólafsdóttir2012) reported D. baeri and D. spathaceum from G. aculeatus. The authors differentiated the site in the eye of the metacercariae; thus, Blair (Reference Blair1973) specified that he found Diplostomum in the eye lens, in the retina, and in the brain of G. aculeatus from different sampling places. Frandsen et al. (Reference Frandsen, Malmquist and Snorrason1989) noted that they found all metacercariae in the vitreous humour of the eyes of Arctic charr, whereas Karvonen et al. (Reference Karvonen, Kristjánsson, Skúlason, Lanki, Rellstab and Jokela2013) identified the species recovered from the eye humour as D. baeri and those from the eye lens as Diplostomum spp.

Frandsen et al. (Reference Frandsen, Malmquist and Snorrason1989) and Kristmundsson and Helgason (Reference Kristmundsson and Helgason2007) reported Diplostomum sp. as the most prevalent of the helminths they recovered from fishes. Also, Kristmundsson and Richter (Reference Kristmundsson and Richter2009) reported a high prevalence of Diplostomum sp., with the intensity being higher in Arctic charr than in brown trout. However, since probably more species of Diplostomum were involved in those records (metacercariae tend to accumulate in their second intermediate hosts), the prevalence might have appeared higher than for single species.

More realistic estimates of the species diversity of Diplostomum in Iceland were provided by Blasco-Costa et al. (Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014) and Faltýnková et al. (Reference Faltýnková, Georgieva, Kostadinova, Blasco-Costa, Scholz and Skírnisson2014), who found a surprisingly high species richness within the genus Diplostomum in Ampullaceana balthica, Arctic charr, brown trout, and three-spined stickleback. In their complex analyses of the isolates of cercariae and metacercariae, integrating molecular, morphological, and ecological data, the authors revealed that there were D. spathaceum and five putative new species/lineages of Diplostomum present in Iceland (see Table 1). This diversity even exceeded that in central Europe reported by Georgieva et al. (Reference Georgieva, Soldánová, Pérez-del-Olmo, Dangel, Sitko, Sures and Kostadinova2013a), who found fewer species in corresponding fish hosts (see Blasco-Costa et al. Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014). Except for D. spathaceum, all species/lineages were unknown to science, and despite the thorough molecular and morphological characterisation of their larval stages, they are awaiting formal description by matching with adults via molecular genetic analyses. Recently, Achatz et al. (Reference Achatz, Martens, Kostadinova, Pulis, Orlofske, Bell, Fecchio, Oyarzún-Ruiz, Syrota and Tkach2022) could match the Icelandic Diplostomum sp. Lin2I with D. rauschi from their material of adults from Chroicocephalus genei (Breme) and Hydroprogne caspia (Pallas) in Ukraine. However, so far, no adult diplostomids have been recorded from Iceland. The Icelandic species/lineages of Diplostomum corresponded to those from snails and fish in Finland, Germany, Lithuania, and Norway recorded by Behrmann-Godel (Reference Behrmann-Godel2013), Georgieva et al. (Reference Georgieva, Soldánová, Pérez-del-Olmo, Dangel, Sitko, Sures and Kostadinova2013a), Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017), and Kudlai et al. (Reference Kudlai, Rakauskas, Baker, Pantoja, Lisitsyna and Binkienė2024), and to those from Canada recovered by Galazzo et al. (Reference Galazzo, Dayanandan, Marcogliese and McLaughlin2002) and Locke et al. (Reference Locke, McLaughlin, Dayanandan and Marcogliese2010a, Reference Locke, McLaughlin and Marcoglieseb) (see Figure 2).

Blasco-Costa et al. (Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014) and Faltýnková et al. (Reference Faltýnková, Georgieva, Kostadinova, Blasco-Costa, Scholz and Skírnisson2014) with their analytical approaches have set a solid framework for assessing the diversity and transmission patterns of Diplostomum spp. in high latitudes. They provided well-characterised and genetically distinct lineages linked to their morphological and ecological features (differing in size, microhabitat in eye, and host use), and thus, they aid to some degree identification based on microhabitat in fish eye. This highlights the importance to process diplostomid metacercariae in a live state from fresh fishes and to precisely record their site of infection in the eye (Faltýnková et al. Reference Faltýnková, Kudlai, Pantoja, Yakovleva and Lebedeva2022), a character already noticed by authors in the past (see above).

All of the Diplostomum spp. occurred at high prevalence and infection intensity in fishes, and those found as cercariae were all recovered in a single snail species, A. balthica. From the high prevalence and continuous occurrence in both snails and fishes throughout the years of sampling, it can be inferred that all species are completing their life cycles within Iceland.

Echinostomatidae

The first record of echinostomes was that of Brinkmann (Reference Brinkmann1956), who reported Echinostoma revolutum and Echinoparyphium recurvatum in anatid birds from Lake Mývatn (see Table 1). Later, Blair (Reference Blair1973) recorded E. recurvatum and Hypoderaeum conoideum as metacercariae in Ampullaceana balthica from south Iceland, which he verified by experimental completion of their life cycles; also, he found echinostome cercariae in A. balthica which remained unidentified.

Much later, Neopetasiger islandicus, the first echinostome species described as new from the territory of Iceland by Kostadinova and Skírnisson (Reference Kostadinova and Skírnisson2007), was found in the grebe species, Podiceps auritus (L.), from Lake Mývatn. By that time, it was assumed that the occurrence of N. islandicus was restricted to the Icelandic population of P. auritus which was geographically isolated from those in North America (Boulet et al. Reference Boulet, Potvin, Shaffer and Breault2005). The life cycle of N. islandicus was elucidated by Georgieva et al. (Reference Georgieva, Kostadinova and Skírnisson2012) using integrative taxonomy, and the life-cycle stages were matched via DNA sequence data.

Two cryptic species of the ‘revolutum’ complex, E. revolutum sensu stricto and another species yet to be formally described, Echinostoma sp. IG, have been characterised via integrative taxonomy by Georgieva et al. (Reference Georgieva, Selbach, Faltýnková, Soldánová, Sures, Skírnisson and Kostadinova2013b) based on material from snails in Iceland and Germany. Echinostoma revolutum s. str. is well known from mainland Europe, where it occurs in Lymnaea stagnalis (L.), but also in other lymnaeid snails, thus having the widest first intermediate host range among the ‘revolutum’ complex (Faltýnková et al. Reference Faltýnková, Našincová and Kablásková2007; Georgieva et al. Reference Georgieva, Faltýnková, Brown, Blasco-Costa, Soldánová, Sitko, Scholz and Kostadinova2014). Georgieva et al. (Reference Georgieva, Selbach, Faltýnková, Soldánová, Sures, Skírnisson and Kostadinova2013b) proved that samples from North America, previously identified as E. revolutum by Detwiler et al. (Reference Detwiler, Bos and Minchella2010), represent another cryptic species of the ‘revolutum’ group, thus indicating that the supposed cosmopolitan distribution of ‘E. revolutum’ might be a result of cryptic variation (Georgieva et al. Reference Georgieva, Selbach, Faltýnková, Soldánová, Sures, Skírnisson and Kostadinova2013b).

To further contribute to the resolution of echinostome species diversity, Pantoja et al. (Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021) examined freshwater snails in Iceland and in other northern regions in Europe and North America (Alaska). In Iceland, almost the same species spectrum as in the past was recorded, with one additional species not found before, Echinoparyphium sp. 2. ex Physa acuta, which was identical to an isolate from Physa gyrina Say in Alberta, Canada of Gordy and Hannington (Reference Gordy and Hanington2019). As echinostome cercariae have been found repeatedly in the lakes over the years, it can be assumed that their life cycles are well established in Iceland (Georgieva et al. Reference Georgieva, Kostadinova and Skírnisson2012; Pantoja et al. Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021). Since Iceland lies on the East Atlantic flyway and is a nesting place for birds overwintering in Britain, Ireland, Norway, Netherlands, and other regions, it is unsurprising that the trematode species are shared with mainland Europe. On the other side, N. islandicus and Echinoparyphium sp. 2, using snails introduced from North America, might be of American origin.

Still, a more extensive assessment of the snail host-range of echinostomes via integrative taxonomy is needed and will possibly lead to more discoveries of trematode specificity and transmission pathways, further helping to reveal ecological patterns in these host-parasite interactions (Pantoja et al. Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021). Because of substantial interspecific homogeneity of morphological features of the life-cycle stages of the echinostomes, considerable taxonomic expertise is needed to distinguish the species morphologically, therefore, it is necessary to combine morphology with molecular genetic analyses for reliable identification (Faltýnková et al. Reference Faltýnková, Georgieva, Soldánová and Kostadinova2015; Georgieva et al. Reference Georgieva, Faltýnková, Brown, Blasco-Costa, Soldánová, Sitko, Scholz and Kostadinova2014).

Plagiorchiidae

In Iceland, there have been scarce records of the Plagiorchiidae. Brinkmann (Reference Brinkmann1956) found a plagiorchiid in the gull, Chroicocephalus ridibundus (L.), from Lake Mývatn and described it as new under the name Paralepidauchen avium, now a synonym of Plagiorchis avium. However, no further records of this species are known. From gyrfalcons (Falco rusticolus L.), Plagiorchis elegans was reported by Clausen and Gudmundsson (Reference Clausen and Gudmundsson1981), Christensen (Reference Christensen2013) and Christensen et al. (Reference Christensen, Skírnisson and Nielsen2015). The latter authors assumed that the infection with P. elegans could be postcyclic (i.e., that the gyrfalcons got infected by adult worms from their prey). This is more likely, as gyrfalcons prey upon birds and mammals (definitive hosts of Plagiorchis spp.), and plagiorchiids use small aquatic invertebrates as intermediate hosts (Krasnolobova Reference Krasnolobova1987) which are an unlikely diet of gyrfalcons. Skírnisson et al. (Reference Skírnisson, Eydal, Gunnarsson and Hersteinsson1993) recorded P. elegans from the Arctic fox in a coastal area in northern Iceland. They assumed that the foxes could get infected from freshwater sources by ingesting aquatic invertebrates (e.g., snails) hosting plagiorchiid metacercariae; another option could be postcyclic infection as in gyrfalcons. Both options are possible, as Arctic foxes have a varied diet including invertebrates (molluscs, crustaceans, insects, echinoderms), birds, and mammals (Hersteinsson and MacDonald Reference Hersteinsson and MacDonald1996).

A much greater diversity of Plagiorchis was found with the use of molecular genetic methods combined with morphology when examining snail first intermediate hosts. In Ampullaceana balthica, Kudlai et al. (Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021) found five species of Plagiorchis new to Iceland, including one new to science (see Table 1). Interestingly, the recovered species are not unique to Iceland; four of them were reported before from Norway by Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017), and Kudlai et al. (Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021) found that they occur also in Finland and Ireland (see Figure 2). Recently, two of those species were recorded in central Europe by Kundid et al. (Reference Kundid, Pantoja, Janovcová and Soldánová2024). From the quite high prevalence (up to 70% in A. balthica, although variable between sampling sites; see Kudlai et al. Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021) over consecutive years and seasonal occurrence of Plagiorchis spp., it is obvious that their life cycles are being completed in Iceland. All recovered Plagiorchis spp. are using one single snail species, A. balthica, as the first and second intermediate host in Iceland. However, some species showed a wider host spectrum in mainland Europe – for example, Plagiorchis sp. 7 sensu Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017) was found also in Myxas glutinosa (O. F. Müller) in Finland.

No overlap in spatial distribution of species of Plagiorchis was noticed between Europe and North America; the records rather reflect a connection between Iceland, Norway, Ireland, and the British Isles within the East Atlantic bird flyway (Cramp and Simmons Reference Cramp and Simmons1983; Kudlai et al. Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021). For P. elegans, which was reported before from North America (e.g., Rausch et al. Reference Rausch, Fay and Williamson1990), Kudlai et al. (Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021) found no match of their cox1 sequences to those from North America of Moszczynska et al. (Reference Moszczynska, Locke, McLaughlin, Marcogliese and Crease2009), Rudko et al. (Reference Rudko, Reimink, Froelich, Gordy, Blankespoor and Hanington2018), and Gordy and Hannington (Reference Gordy and Hanington2019), indicating that this species is most probably restricted to the Palaearctic. Plagiorchis elegans was not found in snails in Iceland but was recorded in continental Europe in Lymnaea stagnalis (see Kudlai et al. Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021; Kundid et al. Reference Kundid, Pantoja, Janovcová and Soldánová2024). Thus, P. elegans might not complete its life cycle in Iceland, and the foxes and gyrfalcons (see above) could possibly have acquired their infections from migratory birds which got infected in mainland Europe. We cannot exclude that some of the species found by Kudlai et al. (Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021) might represent P. avium, recorded before by Brinkmann (Reference Brinkmann1956) (see above). However, further trematode sampling would be appropriate (including other hosts than snails) to assess the species diversity of Plagiorchis in Iceland at a finer scale.

Plagiorchiids are highly adaptable, with adults of low host specificity and a three-host life cycle involving a wide spectrum of second intermediate hosts (aquatic insects and their larvae, crustaceans, molluscs), thus being able to infect a wide spectrum of definitive hosts (birds, bats, and other insectivore mammals) (Krasnolobova Reference Krasnolobova1987; Tkach et al. Reference Tkach, Pawlowski and Sharpilo2000). The recovered species are highly uniform in their morphology, and despite some variability within each species, identification based on morphology is highly unreliable; therefore, DNA sequence data are required to confirm the species identity.

Kundid et al. (Reference Kundid, Pantoja, Janovcová and Soldánová2024), who investigated the species diversity of Plagiorchis in central Europe, found out that so far, the highest species diversity of this genus was recorded in northern Europe. However, in central and more southern Europe, data on trematodes are missing or are not supported by molecular genetic data. Therefore, sampling in more southern regions of Europe is necessary to assess the trematode diversity in lymnaeid snails to find out if the diversity of Plagiorchis spp. is specific to regions in the sub-Arctic or not.

Strigeidae

Adult strigeids of Cotylurus cornutus were recorded in anatid birds by Brinkmann (Reference Brinkmann1956) from Lake Mývatn. Larval stages (cercariae and metacercariae) were found by Blair (Reference Blair1973) in Ampullaceana balthica and Gasterosteus aculeatus in southern Iceland (see Table 1). Blair (Reference Blair1973) verified the identity of his records by experimental completion of the trematode life cycles (i.e., he obtained adults with characters sufficient for identification, a time-costly task requiring meticulous work). In this way, Blair (Reference Blair1973) could identify cercariae and metacercariae of Apatemon gracilis and C. cornutus.

In surveys of fish parasites and investigations on their impact on fish health, strigeid metacercariae identified either as A. gracilis or Apatemon sp. have been recorded in Anguilla anguilla, Gasterosteus aculeatus, Salmo trutta, and Salvelinus alpinus by Richter (Reference Richter1982, Reference Richter2003), Natsopoulou et al. (Reference Natsopoulou, Pálsson and Ólafsdóttir2012), Kristmundsson and Richter (Reference Kristmundsson and Richter2003, Reference Kristmundsson and Richter2009), Karvonen et al. (Reference Karvonen, Kristjánsson, Skúlason, Lanki, Rellstab and Jokela2013), and Sæmundsdóttir (Reference Sæmundsdóttir2023). Apart from A. gracilis, another species of Apatemon, Apatemon sp. 6 was found in planorbid snails of Gyraulus parvus by Faltýnková et al. (Reference Faltýnková, Kudlai, Pantoja, Jouet and Skírnisson2023). The cercariae of Apatemon sp. 6 exhibited prey-mimetism (i.e., a morphological and behavioural adaptation to enhance transmission to the next intermediate fish host by mimicking its prey). The strong morphological adaptation of the cercariae precluded certain identification in the past, but with molecular genetic methods, it was found with certainty that this species belongs to the strigeid genus Apatemon.

The attention of Icelandic authors had been turned on the parasite fauna of gyrfalcons (Falco rusticolus), protected birds of prey, to examine their health state, and a trematode-poor fauna was recorded (Clausen and Gudmundsson Reference Clausen and Gudmundsson1981). Christensen (Reference Christensen2013) and Christensen et al. (Reference Christensen, Skírnisson and Nielsen2015) carried out a large-scale survey of Icelandic gyrfalcons and found few marine species, and several specimens of Strigea sp., which by that time could not be further identified.

The identity of Strigea sp. found by Christensen (Reference Christensen2013) and Christensen et al. (Reference Christensen, Skírnisson and Nielsen2015) was solved recently by integrative taxonomic methods when Faltýnková et al. (Reference Faltýnková, Jouet, Nielsen and Skírnisson2024) obtained DNA sequence data linked to morphology and confirmed the species identity as Strigea falconis. Iceland is most probably the northernmost distributional range of S. falconis, which is a common parasite of birds of prey and is widely distributed in the Holarctic (Dubois Reference Dubois1968; Faltýnková et al. Reference Faltýnková, Jouet, Nielsen and Skírnisson2024; Heneberg et al. Reference Heneberg, Sitko, Těšínský, Rząd and Bizos2018). However, the occurrence of S. falconis in Iceland is surprising, as the gyrfalcon population is not leaving the territory of Iceland, and there are no second and third intermediate hosts (tadpoles, frogs, or reptiles) present to ensure completion of the trematode life cycle. The only plausible answer to this paradox is the annual presence of migratory birds potentially carrying metacercariae of S. falconis acquired in more southern regions of Europe (see Faltýnková et al. Reference Faltýnková, Jouet, Nielsen and Skírnisson2024). The record shows the versatility of this trematode with a complex four-host life cycle, with probably paratenic bird hosts included. However, it also points at Iceland as a dead-end locality for S. falconis. Moreover, gyrfalcons do not seem to be suitable hosts for trematodes because of their restricted range (Arctic, sub-Arctic) and their main diet being rock ptarmigan (Lagopus muta (Montin)), which is not a permissive host for trematodes (Christensen et al. Reference Christensen, Skírnisson and Nielsen2015; Faltýnková et al. Reference Faltýnková, Jouet, Nielsen and Skírnisson2024).

So far, of the family Strigeidae, only for S. falconis and Apatemon sp. 6 DNA sequence data were provided which could be linked to morphological descriptions. Although A. gracilis seems to be a common species in Iceland, a precise identification based on integrative taxonomic methods is still pending. An exact delimitation is needed, as A. gracilis exhibits a high morphological variability and has a wide distributional range, which led to the dispute whether it consists of numerous subspecies or it is a cosmopolitan species with a polytypic morphology and a wide host range (Beverley-Burton Reference Beverley-Burton1961; Dubois Reference Dubois1968). Also, for species of the genera Australapatemon and Cotylurus, molecular genetic data are lacking, indicating that the species diversity of strigeids is not yet fully discovered.

Life cycles of trematodes in Iceland

The life cycles of trematodes are complex, typically involving sequential use of several hosts (vertebrate and invertebrate) linked together mostly by trophic interactions; due to this, trematode diversity and abundance observed in an ecosystem are inherently linked to host diversity and abundance and reflect the dynamics of the trophic interactions of the hosts (Hechinger and Lafferty Reference Hechinger and Lafferty2005). From the data obtained in Iceland by, e.g. Kolářová et al. (Reference Kolářová, Skírnisson, Ferté and Jouet2013), Blasco-Costa et al. (Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014), Kudlai et al. (Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021), or Pantoja et al. (Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021), it is obvious that trematodes can complete their life cycles in freshwater ecosystems in the sub-Arctic. From our data, we have evidence that larval stages do overwinter in snails in Iceland, as there are infections found in snails in June, which is the start of the season, and before this, the water is not warm enough to ensure sufficient parasite development within snails.

Trematodes are highly adaptable, and despite the life-cycle bottleneck (i.e., low host diversity as outlined by Blasco-Costa et al. (Reference Blasco-Costa, Faltýnková, Georgieva, Skírnisson, Scholz and Kostadinova2014)), they are abundant in Iceland. It can be inferred that this substantial trematode diversity is sustained by the definitive bird hosts. Iceland is an important nesting place for aquatic birds, which occur at high density during summer (Jóhannesdóttir et al. Reference Jóhannesdóttir, Arnalds, Brink and Gunnarsson2014; Wetlands International 2006), thus enhancing trematode transmission. Many of those birds are migratory, wintering in Norway, British Isles, Ireland, or France (Cramp and Simmons Reference Cramp and Simmons1983), and this is reflected in the occurrence of conspecific trematode species (Diplostomum, Plagiorchis, and Trichobilharzia) in these countries (Jouet et al. Reference Jouet, Skírnisson, Kolářová and Ferté2010b; Kudlai et al. Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021; Pantoja et al. Reference Pantoja, Faltýnková, O’Dwyer, Jouet, Skírnisson and Kudlai2021; Soldánová et al. Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017) (see Figure 2). An interesting phenomenon is the use of predominantly one snail species, Ampullaceana balthica, as the first intermediate host by diverse trematode species. In other sub-Arctic regions in Canada and Norway, the same pattern of host use and higher-than-expected trematode species diversity was discovered by Gordy and Hanington (Reference Gordy and Hanington2019) and Soldánová et al. (Reference Soldánová, Georgieva, Roháčová, Knudsen, Kuhn, Henriksen, Siwertsson, Shaw, Kuris, Amundsen, Scholz, Lafferty and Kostadinova2017).

In the sub-Arctic freshwater ecosystems, there is a high annual cyclicity of processes, and the free-living fauna are adapted to this by high food consumption and rapid conversion to storage of lipids during summer (Wrona and Reist Reference Wrona, Reist and Meltofte2013). We infer that in Iceland, trematodes are following this pattern, which manifests in intense growth and replication of larval trematode stages in snails during the summer season to match the high abundance of bird hosts, which lasts for a short time. We observed a high prevalence of trematode larval stages in A. balthica, reaching up to 70–80% in August; also, many double infections occur (our data, not shown). In Plagiorchis spp. we noted a tendency to abbreviate the life cycle (i.e., cercariae transform to metacercariae directly in sporocysts, while some cercariae still emerge from the snails). This could be an adaptation to the ephemeral presence of migratory birds stopping for a short time in some of the Icelandic lakes, or it could be an adaptation to a lower diversity/availability of invertebrates other than snails used as second intermediate hosts (Galaktionov and Blasco-Costa Reference Galaktionov and Blasco-Costa2018; Kudlai et al. Reference Kudlai, Pantoja, O’Dwyer, Jouet, Skírnisson and Faltýnková2021).

However, there are records of trematodes from migratory birds where it is clear they do not circulate in Iceland as is the case of Allobilharzia visceralis (Kolářová et al. Reference Kolářová, Rudolfová, Hampl and Skírnisson2006). Also, some species, though recorded in Iceland, as is the case of Strigea falconis, cannot complete their life cycles because of the absence of intermediate hosts (see Faltýnková et al. Reference Faltýnková, Jouet, Nielsen and Skírnisson2024).