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Effect of vancomycin-coated tympanostomy tubes on methicillin-resistant Staphylococcus aureus biofilm formation: in vitro study

Published online by Cambridge University Press:  08 January 2010

C H Jang ,
H Park ,
Y B Cho  and
C H Choi
C H Jang*
Affiliation:
Department of Otolaryngology, Chonnam National University Medical School, South Korea Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
H Park
Affiliation:
Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
Y B Cho
Affiliation:
Department of Otolaryngology, Chonnam National University Medical School, South Korea
C H Choi
Affiliation:
Department of Otolaryngology, Research Center for Resistant Cells, Chosun University, Gwangju, South Korea
*
Address for correspondence: Dr Chul Ho Jang, Department of Otolaryngology, Chonnam National University Medical School, Hakdong 8, Dongku, South Korea. Fax: +82 62 2206776 E-mail: chulsavio@hanmail.net
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Abstract

Background and objective:

Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. It has been suggested that the tube material may be an important factor in the persistence of such otorrhoea. Development of methicillin-resistant Staphylococcus aureus otorrhoea after tympanostomy tube placement is a growing concern. We evaluated the effect of using vancomycin and chitosan coated tympanostomy tubes on the incidence of methicillin-resistant Staphylococcus aureus biofilm formation in vitro.

Materials and methods:

Three sets each of vancomycin-coated silicone tubes (n = 5), commercial silver oxide coated silicone tubes (n = 5) and uncoated tympanostomy tubes (as controls; n = 5) were compared as regards resistance to methicillin-resistant Staphylococcus aureus biofilm formation after in vitro incubation.

Results:

Scanning electron microscopy showed that the surfaces of the silver oxide coated tubes supported the formation of thick biofilms with crusts, comparable to the appearance of the uncoated tubes. In contrast, the surface of the vancomycin-coated tympanostomy tubes was virtually devoid of methicillin-resistant Staphylococcus aureus biofilm.

Conclusion:

Vancomycin-coated tympanostomy tubes resist methicillin-resistant Staphylococcus aureus biofilm formation. Pending further study, such tubes show promise in assisting the control of methicillin-resistant Staphylococcus aureus biofilm formation.

Keywords

Middle EarVentilation TubesOtorrheaMethicillin-Resistant Staphylococcus Aureus

Information

Type
Review Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 6 , June 2010 , pp. 594 - 598
Copyright
Copyright © JLO (1984) Limited 2010

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