Growth balance (proliferation and apoptosis)

Many studies, using a variety of techniques, have measured the magnitude of proliferation in TDLUs and PBL (Table 1). In TDLUs, proliferation averages about 2% and in premenopausal women, the rate fluctuates with the menstrual cycle. In HULs one preliminary study reported an average rate of about 5%, which is similar to the observed rates in ADH [5]. There is substantial data that proliferation is proportional to differentiation along the histological continuum in DCIS with rates averaging as low as 1% in the low-grade to more than 70% in the high-grade lesions [6]. The average proliferation rate in all DCIS combined is about 15%.

Table 1. Summary of biological features of PBL assessed by IHC.

The data on apoptosis, the other side of the growth equation is very limited (Table 1). There is preliminary report that the apoptosis rate is about 0.6% in TDLUs and 0.3% in ADH [7]. In DCIS, apoptosis seems to vary with histological differentiation in DCIS, being much lower in low-grade (1%) than high-grade lesions (5%) [8]. Hence, it appears that the growth of PBLs is likely due to both increased proliferation and decreased cell death and this equilibrium is probably controlled by a variety of regulatory mechanisms, such as sex hormones, oncogenes, tumour suppressor genes, and many other as yet unknown genetic and epigenetic abnormalities.

Oestrogen receptor

Oestrogen, via the ER, plays a central role in regulating the growth and differentiation of normal breast epithelium. For women of all ages combined, an average of about 30% of cells in normal TDLUs express ERα (Table 1) and in premenopausal women, the rate is somewhat lower, about 20%, and varies with the menstrual cycle [9]. The average proportion of ERα-positive cells in TDLUs in postmenopausal women is about 50–60%. In HULs, preliminary data suggests that a large majority express the receptor in over 90% of cells. In ADH >90% of lesions express very high levels of ERα in nearly all cells. In DCIS, overall 75% of all cases express ERα and its expression varies with histological differentiation in DCIS, being highest in low-grade lesions (90–100%), and lowest in high-grade lesions (0–30%) [10].

The very high levels of ERα observed in nearly all premalignant lesions may contribute to their increased proliferation relative to normal cells by allowing them to respond more effectively to any level of oestrogen, even the low concentrations observed in postmenopausal women. Besides ERα, very little is known about the involvement of other types of hormone receptors in PBL. The results of one recent preliminary report suggest that the level of ERβ is significantly lower in most premalignant lesions compared to normal epithelium, which is opposite to that of ERα, despite the high degree of structural homology between the two receptors [11].

Oncogenes and tumour suppressor genes

erbB-2 plays important role in cell growth, differentiation, adhesion, and motility that could contribute to the ability of tumour cells overexpressing erbB-2 to invade and metastasize. Overexpression and amplification of erbB-2 has not been observed in TDLUs or UDH (Table 1). It has been detected only rarely in ADH, although a recent study found amplification of the gene (up to sixfold), in the apparent absence of protein overexpression, in 50% of ADH from breasts containing IBC [12]. In DCIS, many studies have shown that erbB-2 overexpression is rare in low-grade DCIS but seen in 30–40% of high-grade DCIS [13]. Just how alterations of erbB-2 lead to the development and progression of premalignant breast disease is not entirely clear.

Alterations of p53 also appear to play an important role in the evolution of PBL. With the exception of morphologically ‘normal’ breast epithelium in Li–Fraumeni patients with inherited mutations, abnormalities of p53 have not been reported in TDLUs. There are no formal studies of p53 in HULs. A single study looking at p53 in UDH found slightly elevated levels of protein in a small subset of lesions. p53 also appears to be normal in nearly all ADH. p53 in DCIS is quite rare (about 5%) in low-grade lesions, and relatively common (about 40%) in high-grade lesions (Table 1). Mutations of p53 may contribute to the development and progression of premalignant breast disease by several mechanisms including interference with DNA repair through loss of an important G1 cell-cycle checkpoint, leading to replication of a damaged DNA template and genetic instability, and also perhaps by clonal expansion through inhibition of programmed cell death.

Prognostic significance of biological markers in PBL

Only a few studies have evaluated the prognostic value of these markers and have either failed to show any significance or early promising results have not been reproducible.

Allelic imbalances

Most of the genetic abnormalities responsible for the development and progression of PBL are still unknown. Studies of loss of heterozygosity (LOH) analysis or comparative genomic hybridization (CGH) and many other methodologies over the past decade provide crude but compelling evidence that IBC evolves from premalignant lesions by highly diverse genetic and epigenetic mechanisms. These studies have assessed allelic imbalance (AI) to identify the general chromosomal locations of tumour suppressor genes (through losses) or amplified oncogenes (through gains) that may be important in the evolution of PBL (Table 2).

Table 2. Summary of AI (gains and losses) in PBL from studies assessing LOH and CGH.

An interesting study noted that histologically normal TDLUs shared LOH with closely adjacent IBC, while TDLUs farther away in the same breast did not, demonstrating that even normal appearing epithelium may have genotypic abnormalities associated with an elevated risk for developing breast cancer [14]. There is only one preliminary study in a subset of HULs that identified losses at 9q, 17p, and 17q [15]. A few studies in UDH found about 30% AIs involving at least 10 loci on 8 chromosomes. Studies in ADH have shown that up to 50% contain one or more AIs at 30 genetic loci distributed over 10 chromosomes. Studies have shown that nearly all DCIS contain at least one AI among more than 100 genetic loci on 17 chromosomes. Not surprisingly, AIs are more common and numerous in DCIS, especially high-grade lesions than in hyperplasias, consistent with the notion that they represent a relatively late stage of evolution [10].

To identify genetic changes that may be important in progression to IBC, several studies have compared genetic profile between pure DCIS and DCIS with synchronous IBC. Following this strategy, the loci that seem to be important favouring progression include 2q (10–40%), 11p (20–70%), 13q (10–40%), and 17q (5–40%), and those that may protect against progression include 13q and 17q.

Preliminary data from gene expression profiling technology

Many of the innovative high-throughput technologies such as gene expression profiling require fresh/frozen tissue, so the fact that fixed-archival tissue is still the primary resource available for normal and most PBLs remains a problem, but a few recent studies relying on small collections of frozen samples have demonstrated the enormous potential of these new tools to advance our understanding of premalignant evolution.

One of the studies used SAGE to compare the expression of 16 000 gene transcripts in normal epithelium, DCIS, and IBC and found dramatic differences in expression profiles between normal and DCIS but noted that profiles in DCIS and IBC are highly similar [16]. For example, only 16 genes were expressed at significantly different levels in DCIS compared to IBC, 5 being relatively upregulated in DCIS and 11 relatively upregulated in IBC. Among these genes, upregulation of S-100A7/psorisin in high-grade DCIS has been confirmed using reverse transcriptase-polymerase chain reaction (RT-PCR) also.

A few recent preliminary studies have used DNA microarrays to study gene expression in the evolution of PBLs. One such study compared nearly 6000 transcripts between 10 cases of low and high-grade DCIS, and identified 69 genes that were expressed at significantly different levels between the two groups [17]. Another study compared 12 000 transcripts in a larger cohort of samples including ADH, DCIS, and IBC and found gene expression profiles were found to be highly similar in DCIS and IBC, but a signature of 85 genes separated DCIS and IBC [18]. The overall patterns of gene expression were more different between histological grades of the same type of lesions (e.g. low-grade vs. high-grade DCIS) than they were between different stages of lesions (e.g. DCIS vs. IBC), although 85 genes involved in many different biological pathways were expressed at significantly different levels in DCIS compared to IBC. A similar study studied 12 000 transcripts, comparing DCIS and IBC and found more than 200 differentially expressed genes between the two groups. Twenty-six genes of the genes showed from four- to tenfold differences between DCIS and IBC (Table 3) [19].

Table 3. List of putative genes (relatively up- or downregulated in DCIS as compared to IBC) identified by high-throughput expression array studies that are differentially expressed and thus may be involved in the progression of DCIS to IBC.