Introduction
The public has expressed growing concern for how humans treat animals of other species, including fishes. The first research on the welfare of fishes was stimulated by public concern over the conditions faced by food fishes on fish farms in Europe (Kristiansen & Bracke Reference Kristiansen and Bracke2020). Concern has since spread to address fish welfare in other contexts such as biomedical research and commercial and recreational fishing (for reviews, see Huntingford et al. Reference Huntingford, Adams, Braithwaite, Kadri, Pottingers, Sandoe and Turnbull2006; Branson Reference Branson2008; Kristiansen et al. Reference Kristiansen, Fernö, Pavlidis and van de Vis2020). Concern over animal welfare goes beyond physical health and includes consideration of an animal’s psychological and social well-being (Veasey Reference Veasey2017). Studies on well-being of fishes often investigate aspects of confinement such as space limitation, environmental complexity, and social group composition (Oldfield & Bonano Reference Oldfield and Bonano2023), and they are typically conducted by observing and quantifying behaviour (Martins et al. Reference Martins, Galhardo, Noble, Damsgård, Spedicato, Zupa, Beauchaud, Kulczykowska, Massabuau, Carter, Planellas and Kristiansen2012; Watters et al. Reference Watters, Krebs and Eschmann2021). They seek not only to eliminate negative conditions and experiences, but also to elicit positive experiences (Boissy et al. Reference Boissy, Manteuffel, Jensen, Moe, Spruijt, Keeling and Bakken2007; Balcombe Reference Balcombe2009). Despite the burgeoning of this new research on the welfare of fishes, ornamental fishes kept in home aquaria have been largely overlooked. In an aquarium, a fish may encounter conditions which may have harmful negative effects, or positive beneficial effects, on its well-being (Fife-Cook & Franks Reference Fife-Cook and Franks2019). Several recent reviews have discussed the welfare of aquarium fishes (Walster et al. Reference Walster, Rasidi, Saint-Erne and Loh2015; Stevens et al. Reference Stevens, Croft, Paull and Tyler2017; Torgersen Reference Torgersen2020; Brandao et al. Reference Brandao, Dorigão‐Guimarães, Bolognesi, Gauy, Pereira, Vian and Gonçalves‐de‐Freitas2021), but few studies have analysed their behaviour to assess their well-being (Saxby et al. Reference Saxby, Adams, Snellgrove, Wilson and Sloman2010; Oldfield Reference Oldfield2011; Sloman et al. Reference Sloman, Baldwin, McMahon and Snellgrove2011; Smith & Gray Reference Smith and Gray2011).
One ornamental fish species, the Siamese fighting fish (Betta splendens), is aggressive to conspecifics so pet stores keep individual males isolated in small fishbowls around 0.5 L in volume (Figure 1). Producers keep individuals in volumes even smaller, with researchers recommending 150 mL for permanent holding (Saekhow et al. Reference Saekhow, Thongprajukaew, Phromkunthong and Sae-Khoo2018) and 80 mL for transport (Thongprajukaew et al. Reference Thongprajukaew, Takaeh, Esor, Saekhow, Malawa, Nuntapong and Choodum2023). Organisations such as People for the Ethical Treatment of Animals (PETA) consider this practice to be cruel, and have campaigned to end the practice of keeping Bettas in small bowls (The Herald 2009; PETA 2021). Betta splendens is a tropical freshwater fish native to Thailand. Their natural habitat consists of shallow ponds and pools with thick plant cover and stagnant water, a condition they can tolerate due to their labyrinth organ, which allows them to breathe atmospheric air at the water’s surface. In the wild, male Bettas coexist by forming territories at a density of 1.7 individuals m–2 (Pleeging & Moons Reference Pleeging and Moons2017). The fishbowls in which Bettas are held would seem likely to negatively affect a Betta’s well-being by preventing it from moving about. Most Betta care guides suggest a minimum volume of at least 9.5 to 11 L (e.g. Betta Fish Care Guide 2021; Stanton Reference Stanton2021). However, those recommendations are based purely on personal opinion — there is yet to be any research carried out on the well-being of Bettas. The effect of available space on locomotory behaviour in Bettas has not been investigated.
Photograph showing a male Betta splendens in a small (0.5-L) fishbowl at a retail pet store. Photograph courtesy of Ron Oldfield.
It is also not known whether captive male Bettas would peacefully coexist if provided with space and physical cover approximating their natural territories. Some studies have investigated social behaviour of Bettas held in groups in large aquaria. Goldstein (Reference Goldstein1975) observed social behaviour in Bettas held in a large L-shaped aquarium with 2-m long arms and a total volume > 850 L. In addition to the large volume of water available, the aquarium included rooted vegetation for physical enrichment. Under those conditions, Goldstein established a stable community of Bettas that included both males and females, but aggression resulted in the death of several fish prior to establishment of a stable community. Cain et al. (Reference Cain, Jessen and Flanagan1980) specifically tested the effect of space on aggressive behaviour in male Bettas. They placed pairs of males into three differently sized aquaria: small (3.8 L), medium (28.5 L) and large (75.7 L), and found that neither latency to first display, number of displays, nor duration of displays differed as a function of aquarium size, but the number of attacks was significantly lower in larger aquaria. They concluded that attacks are energetically more expensive to perform and not economically advantageous in larger aquaria where there is an increased probability of escape. Haller and Wittenberger (Reference Haller and Wittenberger1988) and Haller (Reference Haller1994) kept multiple male Bettas together for up to seven days. They observed the formation of stable dominance hierarchies and quantified differences in metabolic costs between dominant and subordinate individuals. None of these studies provided fish with territorial densities approximating that found in nature.
In the current study, we set out to determine the minimum amount of space and environmental enrichment required for a lone male Betta to express unreduced rates of swimming behaviour and the amount of space and enrichment required for two male Bettas to coexist peacefully. To this end, two experiments were conducted to determine the effects of space and enrichment on locomotory behaviour and social behaviour. In Experiment 1, we quantified swimming behaviour performed by lone Bettas in small fishbowls and aquaria of various sizes, with and without environmental enrichment, with the expectation that individuals would perform less swimming behaviour in small, unenriched containers. In Experiment 2, we quantified aggressive behaviour in pairs of male Bettas placed in unenriched and enriched aquaria of various sizes. We expected aggression to be lowest in large, enriched aquaria.
Materials and methods
Study animals
Male Bettas were purchased from a retail pet store in Cleveland, Ohio and maintained at Case Western Reserve University in Cleveland, Ohio. The fish were exposed to natural sunlight and the photoperiod of Cleveland, supplemented with standard fluorescent ceiling lights. The amount of time exposed to artificial lighting varied by day. The temperature was kept between 21 and 22°C and fish were fed once daily with either commercial flake food or frozen food (Daphnia or Cyclops spp.).
Experiment 1
In Experiment 1, eight male Bettas were each placed individually in a container of one of four sizes: a round, glass 0.5-L fish bowl (5-cm radius), a 10-L glass aquarium (31 × 21 × 16 cm; length × width × height), a 38-L glass aquarium (51 × 20 × 31 cm), or a 208-L glass aquarium (122 × 33 × 53 cm). Each container size was presented as both an unenriched and an enriched treatment, i.e. there were eight containers set up at any given time, and each trial involved one fish in each of the eight treatments. Unenriched treatments contained only water while enriched treatments included a layer of natural gravel, live unrooted plants (Ceratophyllum demersum), and one large rock that was approximately three-quarters the height of the container and placed in the middle of the container. Water filters were not used. Prior to the commencement of the trials, the fish were moved among the eight containers multiple times with each fish experiencing both an unenriched and an enriched environment at least once before data were collected.
To begin each trial, the water in a particular container was first replaced with aged tap water and then a fish was placed into that container, where it was habituated to the treatment overnight, visually isolated from other fish. The order that each fish experienced each treatment was random. Data collection then began at 1600h the following day. After all data had been collected on any given day, we either immediately moved all the fish to the next treatments (containing new aged tap water) so they could begin habituating for data collection the following day, or we left them in the experimental containers for 2–4 days and then moved them the day preceding the next scheduled day of data collection. This resulted in a habituation period that was consistently 22–26 h across all replicates. Each of the eight fish was observed in each treatment condition one time, once in each of the four unenriched treatments and once in each of the four enriched treatments, for a total of eight observations per fish. Fish were fed only after data recording was finished for the day.
Each fish was observed for 10 min in each treatment. During each 10-min period, each bout of swimming behaviour was recorded. A bout of swimming was defined as locomotory behaviour separated from other locomotory movements by at least one second. To sample all occurrences of swimming behaviour, we used the behaviour sampling rule and the continuous recording rule — each individual was observed continuously, and each observation was uninterrupted (Martin & Bateson Reference Martin and Bateson2007). To calculate time budget, we recorded the behaviour of each individual using the scan sampling rule and the instantaneous point sampling recording rule at 15-s intervals (Martin & Bateson Reference Martin and Bateson2007). These data were used to infer the amount of time each individual spent engaged in swimming behaviour.
To compare the number of swimming bouts performed across individuals, we constructed a Generalized Linear Mixed Model (GLMM) in SPSS 28 using Poisson distribution with log-link function (which fits count data in the form of positive integers). This procedure assumes that multiple records for a single subject represent repeated measurements. The data structure was set with ‘fish’ as subjects and ‘day’ as repeated measures. ‘Container size’, ‘enrichment’, and ‘container size × enrichment’ interaction were set as fixed factors. ‘Container size’ was set as a continuous, scale variable. ‘Subject’ was also included as a fixed factor to control for variation due to individual differences in behaviour. Model fit was assessed by size of Akaike Corrected Information Criterion and Bayesian Information Criterion values. The GLMM function in SPSS is not able to assess simple effects, so to determine which treatments significantly differed from one another, we performed two-way factorial analyses of variance using R version 4.0.3, with ‘swimming’ as the dependent variable, ‘container size’, ‘enrichment’, and ‘container size × enrichment’ interaction as independent variables, and Tukey’s Honest Significant Difference test to identify significant differences between treatments. We performed a similar procedure for time budget data, except in the GLMM we used a normal probability distribution with an identity link function because each data-point was a proportion of total time observed.
Experiment 2
In Experiment 2, we used 16 male Bettas. These individuals differed from those used in Experiment 1, i.e. no fish was used in both Experiment 1 and 2. Each were housed alone in a 38-L glass aquarium (51 × 20 × 31 cm) that contained gravel and live unrooted plants (Ceratophyllum demersum and Lemna minor). Opaque barriers were placed between aquaria so that each Betta was visually isolated from other Bettas > 24 h prior to being subjected to a trial. We held the fish in these tanks for several weeks, and periodically ran preliminary trials in which they were removed and placed in one of the test tanks with another male (see below) to ensure that all fish had prior experience with the treatment conditions by the time trials began.
For each trial, two Bettas were netted from their holding tanks and placed into a glass aquarium of one of four sizes: 10 L (31 × 21 × 16 cm), 38 L (51 × 20 × 31 cm), 208 L (122 × 33 × 53 cm), and 378 L (183 × 46 × 51 cm). The two fish were placed, simultaneously, at opposite ends of the test tank. As in Experiment 1, each aquarium size was presented as both an unenriched and an enriched treatment, i.e. there was a total of eight test aquaria. Unenriched and enriched configurations were the same as in Experiment 1. As in Experiment 1, there were eight different treatments and eight replicates per treatment. However, because each replicate consisted of a pair of fish instead of an individual, fish were specifically chosen for each trial such that no two individuals were paired together more than once. We controlled for the repeated observations of the same individuals by using a repeated measures analysis that included the identity of each individual fish and the day of experimentation as factors in our statistical model (see below). Each pair was then randomly assigned to a particular combination of tank size and enrichment, and this resulted in some individuals experiencing the same treatment more than once. We controlled for prior experience in the same treatment conditions by ensuring that all subjects had prior experience in the various test tanks during preliminary trials (as described above).
Fish were observed for 10 min. All occurrences of behaviour and time budget were recorded as described for Experiment 1, except that we used an ethogram to capture a diversity of behaviour instead of focusing only on swimming (Table 1). Also, time budgets were quantified by recording behaviour of both fish at 30-s instead of 15-s intervals. To prevent injury, the two fish were separated if three bites occurred consecutively (three of 64 replicates had observation periods shorter than 10 min). At the end of each trial, fish were returned to their individual holding tanks and held for 1–3 days until their next trial.
Betta splendens ethogram
To compare numbers of bouts of behaviour performed across treatments, we constructed GLMMs in SPSS 28 using Poisson distribution with log-link function as we did in Experiment 1. As before, ‘container size’ (called ‘aquarium size’ in Experiment 2), ‘enrichment’, ‘aquarium size × enrichment’ interaction, and ‘subject’ were set as fixed factors. Unlike in Experiment 1, in Experiment 2 individuals were not evenly distributed across treatments. Furthermore, Bettas tend to attack aggressive opponents more than non-aggressive ones (Cain et al. Reference Cain, Jessen and Flanagan1980). Therefore, ‘opponent’ was also included as a fixed factor in the models, i.e. each replicate pair was included twice in each analysis: once with one fish designated as the subject and the other fish designated as the opponent, and a second time with the roles reversed. To account for variation in the total minutes of each observation period, we used ln minutes as an offset variable in the model. The model was run one time for each behaviour pattern (Table 1). Outright physical attacks (‘bites’, ‘charges’, and ‘chases’) were scarce, so we summed the bouts of those behaviour patterns to form the new variable ‘total attacks’, which we then analysed using the GLMM. Again, we also performed two-way ANOVAs and Tukey’s Honest Significant Difference tests to determine the significant differences between treatments. We repeated these procedures for time budget data using a normal probability distribution with an identity link function and behaviour per minute as the dependent variable.
Finally, we compared across treatments the latency to perform the first bite. To quantify latency to first bite, each trial was observed for an extra 20 min, to result in a 30-min observation period. If a bite was performed within this period, then the identity of the fish performing the bite and the time at which it occurred were recorded. If no bite occurred during the 30-min period, then that trial was omitted from the data set. Out of the 64 total trials, aggression escalated to biting in 36 trials. We compared latency to first bite across treatments using a GLMM in SPSS 28 using a normal distribution with identity link function. Fixed factors were the same as in the analyses described previously, and latency to first bite was the target (dependent) variable.
Ethical approval
Our experiments were approved by the Institutional Animal Care and Use Committee at Case Western Reserve University (# 2018-0092). Experiment 2 involved placing two individuals together and observing agonistic interactions. Before we ran any trials, we proactively established a procedure that required us to stop any interaction prior to it escalating to ongoing physical combat (i.e. a fight). In contrast to previous studies that allowed ongoing, repeated physical attacks between individuals (e.g. Goldstein Reference Goldstein1975; Cain et al. Reference Cain, Jessen and Flanagan1980), we discontinued any trial by separating the two fish if three ‘bites’ occurred consecutively. No animals were harmed. At the end of the study, fish were either preserved to serve as teaching aids for an ichthyology course or were adopted as pets by students and staff.
Results
Experiment 1
The GLMM that analysed numbers of bouts of swimming found that swimming differed significantly across treatments (Corrected model: F 10,53 = 3.672; P < 0.001). It did not find a significant effect of container size on swimming bouts (F 1,53 = 1.337; P = 0.253) but it did find that enriched environments elicited more swimming bouts than did bare environments (F 1,53 = 14.832; P < 0.001). The model found the ‘container size × enrichment’ interaction not to be significant (F 1,53 = 0.003; P = 0.954). Different subjects were found not to exhibit different rates of swimming (F 7,53 = 1.553; P = 0.170). ANOVA also found number of swimming bouts to be significantly higher in enriched containers overall (F 1,5 = 27.707; P < 0.001), and the Tukey tests revealed several differences between unenriched and enriched treatments (Figure 2[a]).
Showing (a) the mean number of swimming bouts performed by male Betta splendens in a 10-min period and (b) the proportion of time spent swimming in that same period. Means not sharing letters differ significantly according to Tukey HSD (P ≤ 0.021). Error bars show standard error.
The GLMM that analysed time budget found that amount of time spent swimming differed significantly across treatments (Corrected model: F 10,53 = 7.886; P < 0.001). Different subjects were found to exhibit different proportions of time spent swimming (F 7,53 = 4.744; P < 0.001). Even with this difference in behaviour across subjects, the model nevertheless found that behaviour also differed significantly across different container sizes (F 1,53 = 27.134; P < 0.001), but not in bare vs enriched environments (F 1,53 = 0.243; P = 0.624). The model also found the ‘container size × enrichment’ interaction not to be significant (F 1,53 = 0.000; P = 0.990). Furthermore, the ANOVA found that container size had a significant effect on amount of time spent swimming overall (F 1,56 = 21.392; P < 0.001), and Tukey tests revealed that fish spent significantly less time swimming in 0.5-L bowls than they did in the three larger aquaria (Figure 2[b]).
Experiment 2
The statistical output from the GLMM and the ANOVAs used in Experiment 2 are shown in Table 2. The GLMM found that number of bouts of approach behaviour significantly differed across different subjects. The size of the aquarium also affected the number of approaches performed, as indicated by both the GLMM and the ANOVA, and the Tukey tests found that numbers of approaches were significantly lower in the two largest aquarium sizes compared to the 38-L aquaria (Figure 3[a]). The GLMM that tested time spent approaching other fish found an effect for subject, but no other factors affected time spent engaged in approach behaviour according to either the GLMM or the ANOVA (Figure 3[b]).
Results of statistical analyses used in Experiment 2, which tested behaviour in diads of male B. splendens held in bare or enriched aquaria of various sizes
GLMM corrected model d.f. = 33, 94; individual fish (focal or opponent) d.f. = 15, 94; each additional factor or interaction d.f. = 1, 94. ANOVA aquarium size d.f. = 3, 90; enrichment d.f. = 1, 90; interaction d.f. = 3, 90. P-values < 0.05 are highlighted in bold font.
* The repeated values here are not a mistake. It’s a coincidence.
Showing (a) the mean frequency at which male Betta splendens approached another male held in the same aquarium and (b) the proportion of time male B. splendens spent approaching another male. Means not sharing letters differ significantly according to Tukey HSD (P ≤ 0.002). Error bars show standard error.
The GLMM that analysed bouts of displays found that numbers of bouts differed across subjects. The size of the aquarium also affected the number of displays performed, according to both the GLMM and the ANOVA. Overall, fish performed fewer displays when in larger aquaria (Figure 4[a]). Displays were significantly higher in the 38-L aquaria than they were in the 208- and 378-L aquaria (as well as the 10-L aquaria). The GLMM that analysed time budget found that both the identity of the subject and the identity of the opponent fish affected amount of time that the subject spent displaying. The output also indicated that enrichment affected time spent displaying, but visual inspection of the data suggested that this significance was driven by outlying data in the 38-L enriched treatment (Figure 4[b]). It was not supported by the ANOVA, and we did not consider it further. The GLMM that analysed the combined variable ‘total attacks’ found a significant effect for focal fish, but not for any other factors. Even after combining three different attack patterns, the variable ‘total attack’ still occurred at low rates, and attack behaviour was not considered any further.
Showing (a) the mean frequency at which male Betta splendens aggressively displayed toward another male held in the same aquarium and (b) the proportion of time spent displaying. Means not sharing letters differ significantly according to Tukey HSD (P ≤ 0.045). Error bars show standard error.
Retreat behaviour mirrored display behaviour: The GLMM found that both the identity of the subject and aquarium size affected bouts of retreat behaviour (Figure 5[a]). In addition, the identity of the opponent fish also significantly affected the number of bouts of retreat behaviour performed by the subject. Just as individuals performed fewer displays in larger aquaria, they also performed fewer retreats in larger aquaria. The ANOVA did not find an effect of aquarium size on retreat behaviour, so no Tukey tests were performed. However, the significant effect of aquarium size indicated by the GLMM, and the pattern of fewer retreats in larger aquaria that is visible in the data, suggest that the inability of the ANOVA to detect an effect for aquarium size might be due to its inability to control for variation due to the identity of the subjects and the opponent fish. Therefore, we considered this effect to be authentic and we present the graph in Figure 5(a). Our time budget analysis found no significant effect of aquarium size, enrichment, or the identity of the subject (Figure 5[b]). However, identity of the opponent fish had a strong effect on time spent retreating.
Showing (a) the mean frequency at which male Betta splendens retreated from another male held in the same aquarium (GLMM but not ANOVA found that aquarium size affected bouts of retreat behaviour, so no Tukey tests were performed; see text) and (b) the proportion of time spent retreating. Error bars show standard error.
GLMM found a significant effect of aquarium size on bouts of foraging behaviour. The ANOVA was unable to detect this effect, so no Tukey tests were run. However, visual inspection of the data indicates this effect to be authentic: foraging was clearly more frequent in larger aquaria, so we present the graph as Figure 6. No other variable was found to affect foraging behaviour. Foraging behaviour was very rare in the time budget data (out of 128 fish-trials, there were 126 0-values and only two non-zero values), so we did not perform any analyses of time spent foraging. The GLMM found that different individuals performed significantly different rates of ‘gulping air’ behaviour. However, no other factors were found to affect gulping behaviour, so it was not analysed further. The GLMM that analysed latency to first bite found no significant effects.
The mean frequency at which male Betta splendens performed bouts of foraging while another male was held in the same aquarium. GLMM but not ANOVA found that aquarium size affected bouts of foraging behaviour, so no Tukey tests were performed (see text). Error bars show standard error.
Discussion
This study found that confinement in small bowls reduces swimming behaviour in male Betta splendens. In Experiment 1, fish spent significantly less time swimming in small bowls than they did in the larger aquaria. Bettas are not strong swimmers, and they have been reported to spend time sheltering beneath rocks and plants (Pleeging & Moons Reference Pleeging and Moons2017). In contrast, when our Bettas were placed in large aquaria they spent more than half of their time swimming. Swimming rates were similar across the 10-, 38-, and 208-L aquaria, suggesting that 10 L is sufficient to allow the full expression of swimming behaviour in Bettas, which corroborates advice commonly given to aquarium hobbyists (Betta Fish Care Guide 2021; Stanton Reference Stanton2021). However, other common advice is that bigger aquaria are always better. Although we did not formally quantify space use, we observed that fish swam throughout all the space provided to them, even in the largest aquaria.
Swimming has often been interpreted both as an indicator of wellness and as a beneficial, enjoyable experience in animals (e.g. Marshall et al. Reference Marshall, Deere, Little, Snipp, Goulder and Mayer-Clarke2016), including fishes (Martins et al. Reference Martins, Galhardo, Noble, Damsgård, Spedicato, Zupa, Beauchaud, Kulczykowska, Massabuau, Carter, Planellas and Kristiansen2012). In some contexts, locomotion can indicate poor welfare. In large mammalian carnivores, insufficient space may cause repetitive movement that is often interpreted as indicating poor welfare (Kroshko et al. Reference Kroshko, Clubb, Harper, Mellor, Moehrenschlager and Mason2016). In Atlantic halibut (Hippoglossus hippoglossus) grown in food fish aquaculture, swimming has been interpreted as poor welfare when not enough space was available to rest on the floor due to high stocking density (Kristiansen et al. Reference Kristiansen, Fernö, Holm, Privitera, Bakke and Fosseidengen2004). However, in other contexts locomotion has been interpreted as a positive welfare indicator that might be inhibited by limited space or high densities (Oldfield Reference Oldfield2011; Palstra & Planas Reference Palstra and Planas2011; Marshall et al. Reference Marshall, Deere, Little, Snipp, Goulder and Mayer-Clarke2016). Opportunities for animals to roam, to forage, to hunt, to fight, to seek seclusion or the company of others, to take risks, and to make choices are widely acknowledged as necessary for positive well-being (Veasey Reference Veasey2017). The swimming behaviour observed in male Bettas in the current study appeared normal and not stereotypical or stress-related. Even if Bettas kept in small bowls are physically healthy, and they do not seem stressed, they are nevertheless prevented from fulfilling their capability to swim (Nussbaum Reference Nussbaum2006). We cannot know if our Bettas enjoyed the experience of swimming (Oldfield Reference Oldfield2022). Determining what an animal is feeling has been called “the hardest biological problem of all” (Dawkins Reference Dawkins2017). However, our data show that they will swim if given the opportunity to do so, and when confined in bowls their motivation to swim is not realised. Giving Bettas the benefit of the doubt would ensure that any motivation to swim that they might possess is not being frustrated (Birch Reference Birch2017). Allowing Bettas to swim would also allow them to reap the physiological benefits of exercise (Palstra & Planas Reference Palstra and Planas2011), and it would make them more interesting for pet owners to observe.
Our results also found that Bettas performed more instances of swimming behaviour when they were in aquaria containing 3-D objects than when they were in bare bowls or aquaria. Physical structure is generally considered to provide enrichment for diverse vertebrate species (e.g. Scott & LaDue Reference Scott and LaDue2019), and it is often (but not always) beneficial for captive animals (Jones et al. Reference Jones, Webster and Salvanes2021). In addition to providing cognitive stimulation, physical structure may also reduce stress by providing places to shelter (Näslund & Johnsson Reference Näslund and Johnsson2016), and given the choice, fish may prefer environments enriched with rocks and plants (Jones et al. Reference Jones, Webster and Salvanes2021). In Experiment 1, Bettas in enriched treatments performed more swimming bouts than those in unenriched treatments. Since a new swimming bout was recorded every time the fish stopped swimming for at least one second, a higher number of swimming bouts indicates greater variation in behaviour. This suggests that physical enrichment may improve the welfare of Bettas, as behavioural diversity can be an indicator of positive welfare (Miller et al. Reference Miller, Vicino, Sheftel and Lauderdale2020). Physical enrichment did not increase the number of swimming bouts in the 0.5-L bowls. This may be because the bowls were so small that the reduced amount of gravel and plants only negligibly enriched the environment, or because the added objects diminished the already limited space even further.
In Experiment 2, the numbers of times that fish approached and displayed to one another were lower in larger aquaria. This may suggest that greater available space prevented the fish from noticing one another, or that they were unwilling to expend energy to respond to distant stimuli. These results are consistent with those of Bronstein (Reference Bronstein1981), who found that increased inter-individual distance reduced aggression in physically separated male Bettas. The density provided in our largest treatments (2.4 individuals m–2) approached the 1.7 individuals m–2 density observed in territorial wild Bettas (Pleeging & Moons Reference Pleeging and Moons2017), so we expected that our Bettas might cohabitate peacefully, but they did not. No effect of aquarium size on ‘total attack’ behaviour was noted. This opposes the results of Cain et al. (Reference Cain, Jessen and Flanagan1980), who found that neither latency to first display nor number nor duration of displays varied with aquarium size, but that numbers of attacks were significantly lower in larger aquaria. The explanation for this apparent inconsistency is that Cain et al. (Reference Cain, Jessen and Flanagan1980) defined “attack” as “a lunge and open-mouthed contact by the subject”. This behaviour is what we defined as a ‘bite’, and the occurrence of three consecutive bites resulted in us separating the fish and stopping recording data (also note that the range of aquarium sizes in Experiment 2 was much greater than the range used by Cain et al. Reference Cain, Jessen and Flanagan1980). We also found that latency to first bite was not affected by any factor. We expected that fish with more space and physical impediments would escalate to biting more slowly, and we expected more aggressive subjects to escalate faster. The fact that approaches and displays were lower in larger aquaria, but total attacks and latency to first bite were not, suggests that male Bettas might only be motivated to perform a low-intensity behaviour such as an approach when the distance is short and the metabolic cost of swimming is low, whereas Bettas sufficiently motivated to perform a high-intensity behaviour, such as an attack, will do so even if the cost is high.
Retreat behaviour mirrored display behaviour. Just as display behaviour was lower in larger aquaria, so was retreat behaviour. Furthermore, the identity of the opponent fish had a strong effect on retreat behaviour in the subject, as indicated by both number of bouts and time spent retreating. This is perhaps not surprising, because retreat behaviour should be expected to be a direct result of aggressive behaviour. In contrast, foraging behaviour was more frequent in larger aquaria. This might be due either to the fish perceiving more areas worth investigating for the presence of food, or due to less time being spent in proximity of the opponent fish so more time available to search for food.
Physical enrichment, on the other hand, did not reduce the amount of approach behaviour or agonistic behaviour performed. Presence of physical structure can decrease aggression through a variety of mechanisms. The most obvious being that opaque objects reduce the rate of visual contact between two individuals. Bronstein (Reference Bronstein1983) found male Bettas to be less aggressive when their view of a conspecific was partially obscured by plants, and that they possess only a brief within-fight memory for agonistic behaviour and the location of opponents (Bronstein Reference Bronstein1989). Furthermore, in some fish species, an object can function as a landmark that the fish use as a territorial boundary, which encourages the formation of separate territories, thereby reducing aggression (Itzkowitz Reference Itzkowitz1977; Breau & Grant Reference Breau and Grant2002; Smith Reference Smith2011). Finally, sufficient space and environmental complexity can make it uneconomical to behave aggressively. In juvenile Midas cichlids (Amphilophus citrinellus), Oldfield (Reference Oldfield2011) observed that when a sufficiently large “super complex” aquarium was provided, individuals seemed to ‘turn off’ aggressive behaviour and roam peacefully throughout the aquarium, often in close proximity to one another. Perhaps we did not include enough physical structure in our enriched treatments to elicit such a behavioural change in Betta splendens. Alternatively, domesticated Bettas might not ever ‘turn off’ aggressive behaviour. Bettas have been artificially selected for 1,000 years (Kwon et al. Reference Kwon, Vranken, Hoge, Lichak, Norovich, Francis and Bendesky2022), and perhaps there is some amount of sign stimuli that will stimulate aggressive behaviour regardless of ambient ecological conditions.
The identity of the subject had a significant effect on nearly every behaviour pattern we assessed, even behaviours that were not affected by any of the other factors we measured, such as gulping air. This is not surprising. Although Bettas have a reputation for behaving aggressively, aggression in Bettas is well known to vary greatly among individuals (Cain et al. Reference Cain, Jessen and Flanagan1980), so much so that they are often tested for aggressiveness before experiments so that non-aggressive individuals can be omitted (e.g. Bronstein Reference Bronstein1983). Fortunately, the GLMMs we used were able to account for variation among individuals, so not only were we able to prevent individual variation from confounding our experiments, we were able to learn more about it.
Animal welfare implications
The small fishbowls typically used in pet stores prevent male Betta splendens from performing swimming behaviour that they otherwise perform when sufficient space is provided. Male Bettas require physically enriched aquaria of least 10 L in volume to ensure full expression of swimming behaviour. Large, enriched spaces, however, are insufficient to ensure that multiple males will cohabitate peacefully. It seems unlikely that breeders, wholesalers, and retailers will follow this recommendation due to the higher costs required to house the fish (Saekhow et al. Reference Saekhow, Thongprajukaew, Phromkunthong and Sae-Khoo2018; Thongprajukaew et al. Reference Thongprajukaew, Takaeh, Esor, Saekhow, Malawa, Nuntapong and Choodum2023), but consumers might do so if their goal as pet owners is not to admire their Bettas as trophies but to nurture them to ensure that they thrive to the greatest extent possible.
Acknowledgements
We thank Felicity A Huntingford for first training RO in the study of the behaviour of Betta splendens. We dedicate this study to Paul M Bronstein (deceased), who was an influential mentor to RO at the University of Michigan. Dr Bronstein’s research on the behaviour of Bettas laid the foundation for the current study. We also thank Owen Lockhart for maintaining the facility used to house the fish.
Competing interest
None.
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