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Comparing the genetic structure of codling moth Cydia pomonella (L.) from Greece and France: long distance gene-flow in a sedentary pest species

Published online by Cambridge University Press:  28 October 2011

C.Ch. Voudouris ,
P. Franck ,
J. Olivares ,
B. Sauphanor ,
Z. Mamuris ,
J.A. Tsitsipis  and
J.T. Margaritopoulos
C.Ch. Voudouris
Affiliation:
Department of Biochemistry & Biotechnology, University of Thessaly, Ploutonos 26, 41221 Larissa, Greece
P. Franck
Affiliation:
UR 1115, Plantes et Systèmes de culture Horticoles, INRA. Site Agroparc, 84914 Avignon Cedex 9, France
J. Olivares
Affiliation:
UR 1115, Plantes et Systèmes de culture Horticoles, INRA. Site Agroparc, 84914 Avignon Cedex 9, France
B. Sauphanor
Affiliation:
UR 1115, Plantes et Systèmes de culture Horticoles, INRA. Site Agroparc, 84914 Avignon Cedex 9, France
Z. Mamuris
Affiliation:
Department of Biochemistry & Biotechnology, University of Thessaly, Ploutonos 26, 41221 Larissa, Greece
J.A. Tsitsipis
Affiliation:
Laboratory of Entomology and Agricultural Zoology, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou Str., 38446 Nea Ionia, Greece
J.T. Margaritopoulos*
Affiliation:
Department of Biochemistry & Biotechnology, University of Thessaly, Ploutonos 26, 41221 Larissa, Greece
*
*Author for correspondence Fax: 00302410565290 E-mail: johnmargaritopoulos@gmail.com
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Abstract

Codling moth Cydia pomonella L. (Lepidoptera: Tortricidae) is the most important insect pest of apple production in Europe. Despite the economic importance of this pest, there is not information about the genetic structure of its population in Greece and the patterns of gene-flow which might affect the success of control programs. In this study, we analysed nine samples from apple, pear and walnut from various regions of mainland Greece using 11 microsatellite loci. Six samples from the aforementioned hosts from southern France were also examined for comparison. Bayesian clustering and genetic distance analyses separated the codling moth samples in two genetic clusters. The first cluster consisted mainly of the individuals from Greece, and the second of those from France, although admixture and miss-classified individuals were also observed. The low genetic differentiation among samples within each country was also revealed by FST statistics (0.009 among Greek samples and 0.0150 among French samples compared to 0.050 global value among all samples and 0.032 the mean of the pair-wise values between the two countries). These FST values suggest little structuring at large geographical scales in agreement with previous published studies. The host species and local factors (climatic conditions, topography, pest control programs) did not affect the genetic structure of codling moth populations within each country. The results are discussed in relation to human-made activities that promote gene-flow even at large geographic distances. Possible factors for the genetic differentiation between the two genetic clusters are also discussed.

Keywords

codling mothpopulation structuregene-flowappleTorticidaeLepidoptera

Information

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 2 , April 2012 , pp. 185 - 198
Copyright
Copyright © Cambridge University Press 2011

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Table S1. Single and multilocus probability tests for deviations from HW equilibrium and FIS coefficient values.

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