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Variation in bacterial endosymbionts associated with the date palm hopper, Ommatissus lybicus populations

Published online by Cambridge University Press:  15 August 2017

S. Karimi ,
H. Izadi ,
M. Askari Seyahooei ,
A. Bagheri  and
P. Khodaygan
S. Karimi*
Affiliation:
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran
H. Izadi
Affiliation:
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran
M. Askari Seyahooei
Affiliation:
Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran
A. Bagheri
Affiliation:
Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran
P. Khodaygan
Affiliation:
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran
*
*Author for correspondence Phone: +98 34 31312156 Fax: +98 34 31312155 E-mail: S.karimi@stu.vru.ac.ir
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Abstract

The date palm hopper, Ommatissus lybicus, is a key pest of the date palm, which is expected to be comprised of many allopatric populations. The current study was carried out to determine bacterial endosymbiont diversity in the different populations of this pest. Ten date palm hopper populations were collected from the main date palm growing regions in Iran and an additional four samples from Pakistan, Oman, Egypt and Tunisia for detection of primary and secondary endosymbionts using polymerase chain reaction (PCR) assay with their specific primers. The PCR products were directly sequenced and edited using SeqMan software. The consensus sequences were subjected to a BLAST similarity search. The results revealed the presence of ‘Candidatus Sulcia muelleri’ (primary endosymbiont) and Wolbachia, Arsenophonus and Enterobacter (secondary endosymbionts) in all populations. This assay failed to detect ‘Candidatus Nasuia deltocephalinicola’ and Serratia in these populations. ‘Ca. S. muelleri’ exhibited a 100% infection frequency in populations and Wolbachia, Arsenophonus and Enterobacter demonstrated 100, 93.04 and 97.39% infection frequencies, respectively. The infection rate of Arsenophonus and Enterobacter ranged from 75 to 100% and 62.5 to 100%, respectively, in different populations of the insect. The results demonstrated multiple infections by ‘Ca. Sulcia muelleri’, Wolbachia, Arsenophonus and Enterobacter in the populations and may suggest significant roles for these endosymbionts on date palm hopper population fitness. This study provides an insight to endosymbiont variation in the date palm hopper populations; however, further investigation is needed to examine how these endosymbionts may affect host fitness.

Keywords

Arsenophonusbacterial endosymbiontEnterobacterOmmatissus lybicusSulciaWolbachia

Information

Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 2 , April 2018 , pp. 271 - 281
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Copyright © Cambridge University Press 2017 

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