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Mouse oocytes penetrated by sperm at GV or GVBD stage lose the ablity to fuse with additional spermatozoa

Published online by Cambridge University Press:  26 September 2008

Beata Pyrzyńska ,
M. Maleszewski  and
D. Maluchnik
Beata Pyrzyńska
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
M. Maleszewski
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
D. Maluchnik*
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
*
Marek Maleszewski, Department of Embryology, Institute of Zoology, University of Warsaw, 00-927 Warszawa 64, Poland. Fax: (48 22) 26 86 24. e-mail:embrio@plearn.edu.pl.
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Summary

Mouse oocytes penetrated by spermatozoa during germinal vesicle (GV) breakdown undergo maturation and are arrested at metaphase of the second meiotic division despite the presence of sperm nuclei within the ooplasm. When these oocytes were re-inseminated, none was penetrated by newly added spermatozoa. When GV oocytes were inseminated and cultured in the presence of dibutyryl cAMP, the oocytes remained at GV stage, yet they did not permit entry of additional spermatozoa. These observations suggest that the plasma membrane of maturing oocytes is modified by precociously penetrating spermatozoa independently from cortical granule exocytosis. Sperm components incorporated into the oocytes seem to be responsible for the modification of the oocyte's plasma membrane.

Keywords

Mouse oocyteOocte maturationOolemma block to sperm penetrationIn vitro fertilisationRe-insemination

Information

Type
Article
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 123 - 128
Copyright
Copyright © Cambridge University Press 1996

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References

Austin, C.R. & Braden, A.W.H. (1956). Early reactions of the rodent egg to spermatozoon penetration. J. Exp. Biol. 33, 358–65.Google Scholar
Borsuk, E. & Tarkowski, A.K. (1989). Transformation of sperm nuclei into male pronuclei in nucleate and anucleate fragments of parthenogenetic mouse eggs. Gamete Res. 24,471–81.Google Scholar
Cho, W.K., Stern, S. & Biggers, J.D. (1974). Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J. Exp. Zool. 187, 383–6.Google Scholar
Clarke, H.J. & Masui, Y. (1986). Transformation of sperm nuclei to metaphase chromosomes in the cytoplasm of maturing oocytes of the mouse. J. Cell Biol. 102, 1039–46.CrossRefGoogle ScholarPubMed
Ducibella, T. & Buetow, J. (1994). Competence to undergo normal, fertilization-induced cortical activation develops after metaphase I of meiosis in mouse oocytes. Dev. Biol. 165, 95–104.Google Scholar
Fraser, L.R. (1982). Ca2+ is required for mouse sperm capacitation and fertilization in vitro. J. Androl. 3, 412–19.CrossRefGoogle Scholar
Fulton, B.P. & Whittingham, D.G. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle ScholarPubMed
Gaunt, S.J. (1983). Spreading of a sperm surface antigen within the plasma membrane of the egg after fertilization of the rat. J. Embryol. Exp. Morphol. 75, 259–70.Google ScholarPubMed
Horvath, P.M., Kellom, T., Caulfield, J. & Boldt, J. (1993). Mechanistic studies of the plasma membrane block to polyspermy in mouse eggs. Mol. Reprod. Dev. 34, 65–72.CrossRefGoogle ScholarPubMed
Kimura, Y. & Yanagimachi, R. (1995). Intracytoplasmic sperm injection in the mouse. Biol. Reprod. 52, 1–12.Google Scholar
Komar, A. (1982). Fertilization of parthenogenetically activated mouse eggs. Exp. Cell Res. 139, 361–7.Google Scholar
Maleszewski, M. (1990). Decondensation of mouse sperm chromatin in cell-free extracts: a micromethod. Mol. Reprod. Dev. 27, 244–8.CrossRefGoogle ScholarPubMed
Maleszewski, M. (1992). Behaviour of sperm nuclei incorporated into parthenogenetic mouse eggs prior to the first cleavage division. Mol. Reprod. Dev. 33, 215–21.Google Scholar
Maleszewski, M. & Bielak, A. (1993). Sperm penetration in parthenogenetic mouse embryos triggers a plasma membrane block to polyspermy. Zygote 1, 237–42.CrossRefGoogle ScholarPubMed
Maleszewski, M., Kimura, Y. & Yanagimachi, R. (1995). Oolemma block to polyspermy is not induced in mouse oocytes activated by direct sperm injection. Biol. Reprod. 52(S1), abstr. 255.Google Scholar
Maluchnik, D. & Borsuk, E. (1994). Sperm entry into fertilised mouse eggs. Zygote 2, 129–31.CrossRefGoogle ScholarPubMed
Mehlmann, L.M. & Kline, D. (1994). Regulation of intracellular calcium in the mouse egg: calcium release in response to sperm or inositol triphosphate is enhanced after meiotic maturation. Biol. Reprod. 51, 1088–98.CrossRefGoogle ScholarPubMed
Miller, M.A. & Masui, Y. (1982). Changes in the stainability and sulphydryl level in the sperm nucleus during sperm-oocyte interaction in mice. Gamete Res. 5, 167–79.CrossRefGoogle Scholar
Nicolson, G.I., Yanagimachi, R. & Yanagimachi, H. (1975). Ultrastructural localization of lectin-binding Sites on the zonae pellucidae and plasma membranes of mammalian eggs. J. Cell Biol. 66, 263–74.Google Scholar
Sato, K. (1979). Polyspermy-preventing mechanisms in mouse eggs fertilized in vitro. J. Exp. Zool. 210, 353–9.Google Scholar
Szöllösi, D. (1962). Cortical granules: a general feature of mammalian eggs?. J. Reprod. Fertil. 4,223–4.Google Scholar
Szöliösi, D., Szöllösi, M.S., Czolowska, R. & Tarkowski, A.K. (1990). Sperm penetration into immature mouse oocytes and nuclear changes during maturation: an EM study. Biol. Cell 69, 53–64.Google Scholar
Tarkowski, A.K. (1966). An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 5, 394–400.CrossRefGoogle Scholar
Wasserman, P.M. (1990). Profile of a mammalian sperm receptor. Development. 108, 1–17.Google Scholar
Wolf, D.P. (1978). The block to sperm penetration in zonafree mouse ova. Den. Biol. 64, 1–10.CrossRefGoogle Scholar