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middle ear mucosal regeneration by nasal mucosal epithelial cell sheets transplantation

Presenting Author: Yuichiro Yaguchi

Published online by Cambridge University Press:  03 June 2016

Yuichiro Yaguchi ,
Kazuhisa Yamamoto ,
Tsunetaro Morino  and
Hiromi Kojima
Yuichiro Yaguchi
Affiliation:
St.Marianna University School of Medicine
Kazuhisa Yamamoto
Affiliation:
Jikei University School of Medicine
Tsunetaro Morino
Affiliation:
Jikei University School of Medicine
Hiromi Kojima
Affiliation:
Jikei University School of Medicine
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Abstract

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Learning Objectives:

Postoperative regeneration of the middle ear mucosa and pneumatization of the middle ear cavity are of great importance after middle ear surgery. This study developed a new method to transplant autologous nasal mucosal epithelial cell-sheets into the damaged middle ear cavity. The aim of this study was to evaluate postoperative healing after the transplantation of the cell sheets in rabbits. Rabbit nasal mucosal epithelial cell-sheets were fabricated from a temperature-responsive culture dish and transplanted into the damaged middle ear of rabbit, which was surgically created. The healing of middle ears was evaluated with histological methods and computed tomography findings at 8 weeks after transplantation. Functional evaluation was performed by measuring the maximum middle ear total pressure reflecting a trans-mucosal gas exchange function. Two control groups were used: the normal control group and the mucosa-eliminated control group. Transplantation of nasal mucosal epithelial cell-sheets suppressed the bone hyperplasia and the narrowing of pneumatic space in the middle ear cavity more clearly than the mucosa-eliminated control group. The mucosal gas exchange function was also found to be good in the cell sheet-transplanted group. These results suggested that posttransplanted middle ear cavity was not only morphologically but also functionally similar to the normal middle ear cavity. Nasal mucosal epithelial cell-sheet was confirmed to be useful as an effective graft material after middle ear surgery and hopefully become a novel therapy in the future.