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In Vitro Biocompatibility Testing of Collagen-Calcium Phosphate Composites Using Human Bone Derived Osteoblasts

Published online by Cambridge University Press:  15 February 2011

A.C. Lawson ,
M. Oyama ,
M.E. Emerton ,
M.J.O. Francis ,
A.H.R.W. Simpson  and
J.T. Czernuszka
A.C. Lawson
Affiliation:
Department of Materials, University of Oxford, Oxford, U.K.
M. Oyama
Affiliation:
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Oxford, U.K.
M.E. Emerton
Affiliation:
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Oxford, U.K.
M.J.O. Francis
Affiliation:
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Oxford, U.K.
A.H.R.W. Simpson
Affiliation:
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Oxford, U.K.
J.T. Czernuszka
Affiliation:
Department of Materials, University of Oxford, Oxford, U.K.
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Abstract

Human bone derived osteoblasts were cultured on collagen-calcium phosphate composites. The ability of the substrates to support cell attachment, proliferation and bone formation was assessed using histochemical staining for alkaline phosphatase activity and immunolocalisation of transforming growth factor- β1and type 1 collagen. The effect of calcium phosphate phase and crystal size was investigated and the calcified samples compared with uncalcified collagen. Osteoblasts adhere to the collagen-calcium phosphate composites and express a mature osteoblast phenotype in vitro. Cell adhesion was greater on unmineralised collagen than on the mineralised composites, however, these cells were less differentiated. The presence of larger crystals seemed to have a detrimental effect on the cells, reducing proliferation and alkaline phosphatase activity. There was no discernible difference between the effect of hydroxyapatite and octacalcium phosphate on the cells.

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