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Clonal Diversity of Methicillin-Resistant Staphylococcus aureus in an Acute-Care Institution

Published online by Cambridge University Press:  21 June 2016

L.E. Nicolle ,
H. Bialkowska-Hobrzanska ,
L. Romance ,
VS. Harry  and
S. Parker
L.E. Nicolle*
Affiliation:
Infection Control Unit, Health Sciences Centre, Winnipeg, Manitoba Departments of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba Internal Medicine, University of Manitoba, Winnipeg, Manitoba
H. Bialkowska-Hobrzanska
Affiliation:
St. Joseph's Health Centre, London, Ontario
L. Romance
Affiliation:
Infection Control Unit, Health Sciences Centre, Winnipeg, Manitoba
VS. Harry
Affiliation:
St. Joseph's Health Centre, London, Ontario
S. Parker
Affiliation:
Microbiology Laboratory, Health Sciences Centre, Winnipeg, Manitoba Departments of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba St. Joseph's Health Centre, London, Ontario
*
Health Sciences Centre, MS673-820 Sherbrook St., Winnipeg, Manitoba, R3A 1R9, Canada
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Abstract

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Objective:

To evaluate plasmid and chromosomal typing methods for differentiation of methicillin-resistant Staphylococcus aureus (MRSA).

Design:

Comparison of relatedness of strains using epidemiologic features, phage typing, and antimicrobial susceptibility with blinded assessment by molecular typing methods. Molecular typing methods included AccI and Clal restriction endonuclease fingerprinting of chromosomal DNA and plasmid typing.

Setting:

Tertiary-care teaching hospital.

Methods:

Convenience sample of 10 MRSA strains, including 4 outbreak-associated and 6 sporadic strains of diverse epidemiologic origins without evidence of nosocomial transmission.

Results:

Only 2 strains were phage typeable. The epidemic strain was distinguishable by its susceptibility pattern. The other strains were not consistently separable by phenotyping or plasmid typing methods but were distinct by chromosome typing.

Conclusions:

These observations document the diversity of origins of MRSA strains introduced into an acute-care institution. Chromosomal typing may be the preferred method for the determination of clonal origin of MRSA.

Information

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 13 , Issue 1 , January 1992 , pp. 33 - 37
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1992 

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