Single-Molecule Cellular Biophysics

Mark C. Leake

doi:10.1017/CBO9780511794421

Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.

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Single-Molecule Cellular Biophysics

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Contents

Frontmatter
pp i-vi

Contents
pp vii-x

Preface
pp xi-xiv

1 - Once upon a (length and) time (scale). . .
pp 1-28

2 - The molecules of life – an idiot’s guide
pp 29-59

3 - Making the invisible visible: part 1 – methods that use visible light
pp 60-101

4 - Making the invisible visible: part 2 – without visible light
pp 102-120

5 - Measuring forces and manipulating single molecules
pp 121-148

6 - Single-molecule biophysics: the case studies that piece together the hidden machinery of the cell
pp 149-158

7 - Molecules from beyond the cell
pp 159-182

8 - Into the membrane
pp 183-219

9 - Inside cells
pp 220-252

10 - Single-molecule biophysics beyond single cells and beyond the single molecule
pp 253-264

Index
pp 265-275

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