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Transcriptional identification of differentially expressed genes during the prepupal–pupal transition in the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  22 March 2021

Peirong Li ,
Xinru Li ,
Wei Wang ,
Xiaoling Tan ,
Xiaoqi Wang  and
Xueqing Yang Open the ORCID record for Xueqing Yang [Opens in a new window]
Peirong Li
Affiliation:
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China
Xinru Li
Affiliation:
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China
Wei Wang
Affiliation:
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China
Xiaoling Tan*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Xiaoqi Wang
Affiliation:
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China
Xueqing Yang*
Affiliation:
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China
*
Author for correspondence: Xueqing Yang, Email: sling233@hotmail.com; Xiaoling Tan, Email: tanxiaoling2010@163.com
Author for correspondence: Xueqing Yang, Email: sling233@hotmail.com; Xiaoling Tan, Email: tanxiaoling2010@163.com
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Abstract

The oriental armyworm, Mythimna separata (Walker) is a serious pest of agriculture that does particular damage to Gramineae crops in Asia, Europe, and Oceania. Metamorphosis is a key developmental stage in insects, although the genes underlying the metamorphic transition in M. separata remain largely unknown. Here, we sequenced the transcriptomes of five stages; mature larvae (ML), wandering (W), and pupation (1, 5, and 10 days after pupation, designated P1, P5, and P10) to identify transition-associated genes. Four libraries were generated, with 22,884, 23,534, 26,643, and 33,238 differentially expressed genes (DEGs) for the ML-vs-W, W-vs-P1, P1-vs-P5, and P5-vs-P10, respectively. Gene ontology enrichment analysis of DEGs showed that genes regulating the biosynthesis of the membrane and integral components of the membrane, which includes the cuticular protein (CP), 20-hydroxyecdysone (20E), and juvenile hormone (JH) biosynthesis, were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that DEGs were enriched in the metabolic pathways. Of these DEGs, thirty CP, seventeen 20E, and seven JH genes were differentially expressed across the developmental stages. For transcriptome validation, ten CP, 20E, and JH-related genes were selected and verified by real-time PCR quantitative. Collectively, our results provided a basis for further studies of the molecular mechanism of metamorphosis in M. separata.

Keywords

Gene expressionmetamorphosisMythimna separatapupationtranscriptome analysis

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Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 4 , August 2021 , pp. 485 - 498
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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