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Natural resin acid –enriched composition as a modulator of intestinal microbiota and performance enhancer in broiler chicken

Published online by Cambridge University Press:  04 March 2015

H. Kettunen ,
J. Vuorenmaa ,
T. Rinttilä ,
H. Grönberg ,
E. Valkonen  and
J. Apajalahti
H. Kettunen*
Affiliation:
Alimetrics Ltd, Koskelontie 19B, FI-02920 Espoo, Finland
J. Vuorenmaa
Affiliation:
Hankkija Ltd, Peltokuumolantie 4, FI-05800 Hyvinkää, Finland
T. Rinttilä
Affiliation:
Alimetrics Ltd, Koskelontie 19B, FI-02920 Espoo, Finland
H. Grönberg
Affiliation:
Alimetrics Ltd, Koskelontie 19B, FI-02920 Espoo, Finland
E. Valkonen
Affiliation:
Hankkija Ltd, Peltokuumolantie 4, FI-05800 Hyvinkää, Finland
J. Apajalahti
Affiliation:
Alimetrics Ltd, Koskelontie 19B, FI-02920 Espoo, Finland
*
Corresponding author:hakettunen@hotmail.com
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Summary

Resin acids extracted from coniferous trees are known for their antimicrobial and antifungal effects. This trial investigated the effect of a natural resin acid-enriched composition (RAC) on the gastrointestinal microbiota and productive performance of broiler chicken. The results demonstrated that at or above 5 mg/l, RAC prevented the growth of a pure culture of Clostridium perfringens, a causative agent of necrotic enteritis in poultry. Next, the effects of RAC on the microbial community were studied in a fermentation model with both the microbial inoculum and substrate for the microbes isolated from the ileum of broiler chickens. RAC was included at 0, 0.1 and 1 g/kg digesta, and supplementation decreased the relative proportion of lactic acid and increased that of acetic acid produced during the fermentation in a dose-dependent manner. At 1 g/kg inclusion, RAC decreased the density of lactobacilli. The final part of the experiment investigated the influence of RAC on the performance and intestinal microbiota of necrotic enteritis (NE)-challenged broiler chickens. A wheat and soy -based diet was supplemented with RAC at 0, 0.5, 1 and 3 g/kg. The chickens were challenged with Eimeria maxima oocysts on day 11, and a pure culture of C. perfringens on day 14. On day 17, the final day of the trial, RAC inclusion at 1 and 3 g/kg of feed significantly increased body weight. At 3 g/kg RAC numerically decreased the daily mortality seen during the challenge period. In the ileum, RAC at 1 g/kg reduced the NE-associated peak of microbial lactic acid production. Overall, the data suggested that the dietary ingredient RAC has the potential to act as a performance-enhancer and microbial modulator in broiler chickens.

Keywords

poultryresin acidnecrotic enteritismicrobial profileperformance

Information

Type
Original Research
Information
Journal of Applied Animal Nutrition , Volume 3 , 2015 , e2
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2015 

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References

Ali, F., Sangwan, P.L., Koul, S., Pandey, A., Bani, S., Abdullah, S.T., Sharma, P.R., Kitchlu, S., and Khan, I.A. (2012) 4-epi-Pimaric acid: a phytomolecule as a potent antibacterial and anti-biofilm agent for oral cavity pathogens. European Journal of Clinical Microbiology & Infectious Diseases, 31:149–159.Google Scholar
Allen, P.C., Danforth, H.D., and Augustine, P.C. (1998) Dietary modulation of avian coccidiosis. Internatonal Journal of Parasitology, 28:1131–1140.CrossRefGoogle ScholarPubMed
Apajalahti, J. and Kettunen, A. (2006) Rational development of novel microbial modulators, in: Barug, D., de Jong, J., Kies, A.K., & Verstegen, M.W.A. (Eds). Antimicrobial growth promoters. Where do we go from here? pp. 165–181. (Wageningen Academic Publishers, Wageningen).Google Scholar
Apajalahti, J., Rademacher, M., Htoo, J.K., Redshaw, M, and Kettunen, A. (2009) Divergent modulation of swine ileal microbiota by formic acid and methionine hydroxy analogue-free acid. Animal, 3 817–825.Google Scholar
Feighner, S.D., and Dashkevicz, M.P. (1987) Subtherapeutic levels of antibiotics in poultry feeds and their effects on weight gain, feed efficiency, and bacterial cholyltaurine hydrolase activity. Applied and Environmental Microbiology, 53:331–336.Google Scholar
Gong, J., Si, W., Forster, R.J., Huang, R., Yu, H., Yin, Y., Yang, C., and Han, Y. (2007) 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiology Ecology, 59:147–157.Google Scholar
Huyghebaert, G., Ducatelle, R., and Van Immerseel, F. (2011) An update on alternatives to antimicrobial growth promoters for broilers. Veterinary Journal, 187:182–188.CrossRefGoogle ScholarPubMed
Koluman, A., and Dikici, A. (2013) Antimicrobial resistance of emerging foodborne pathogens: status quo and global trends. Critical Reviews in Microbiology, 39:57–69.Google Scholar
Korver, D.R., Wakenell, P., and Klasing, K.C. (1997) Dietary fish oil or lofrin, a 5-lipoxygenase inhibitor, decrease the growth-suppressing effects of coccidiosis in broiler chickens. Poultry Science, 76:1355–1363.Google Scholar
Lee, K.W., Lillehoj, H.S., Jeong, W, Jeoung, H.Y., and An, D.J. (2011) Avian necrotic enteritis: experimental models, host immunity, pathogenesis, risk factors, and vaccine development. Poultry Science, 90:1381–1390.Google Scholar
Lin, J. (2014) Antibiotic growth promoters enhance animal production by targeting intestinal bile salt hydrolase and its producers. Frontiers in Microbiology, 5:33.Google Scholar
Lunedo, R., Fernandez-Alarcon, M.F., Carvalho, F.M., Furlan, L.R., and Macari, M. (2014) Analysis of the intestinal bacterial microbiota in maize- or sorghum-fed broiler chickens using real-time PCR. British Poultry Science, Oct 31. [Epub. ahead of print] DOI: 10.1080/00071668.2014.975781CrossRefGoogle ScholarPubMed
Milligan, H.T., and Koricheva, J. (2013) Effects of tree species richness and composition on moose winter browsing damage and foraging selectivity: an experimental study. Journal of Animal Ecology, 82:739–748.CrossRefGoogle ScholarPubMed
Nadkarni, M.A., Martin, F.E., Jacques, N.A., and Hunter, N. (2002) Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology, 148:257–266.Google Scholar
Rinttilä, T., Kassinen, A., Malinen, E., Krogius, L., and Palva, A. (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in fecal samples by real-time PCR. Journal of Applied Microbiology, 97:1166–1177.CrossRefGoogle ScholarPubMed
Rubio, J., Calderón, J.S., Flores, A., Castroa, C., and Céspedes, C.L. (2005) Trypanocidal activity of oleoresin and terpenoids isolated from Pinus oocarpa. Zeitschrift für Naturforschung C, 60:711–716.Google Scholar
San Feliciano, A., Gordaliza, M., Salinero, M.A., and Miguel del Corral, J.M. (1993) Abietane acids; Sources, Biological Activities, and Therapeutic Uses. Planta Medica, 59:485–490.Google Scholar
Savluchinske-Feio, S., Curto, M.J., Gigante, B., and Roseiro, J.C. (2006) Antimicrobial activity of resin acid derivatives. Applied Microbiology and Biotechnology, 72:430–436.CrossRefGoogle ScholarPubMed
Shojadoost, B., Vince, A.R., and Prescott, J.F. (2012) The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens: a critical review. Veterinary Research, 43:74.Google Scholar
Sipponen, A., Kuokkanen, O., Tiihonen, R., Kauppinen, H., and Jokinen, J.J. (2012) Natural coniferous resin salve used to treat complicated surgical wounds: pilot clinical trial on healing and costs. International Journal of Dermatology, 51:726–732.Google Scholar
Sipponen, A., and Laitinen, L. (2011) Antimicrobial properties of natural coniferous rosin in the European Pharmacopoeia challenge test. APMIS, 119:720–724.Google Scholar
Smith, E., Williamson, E., Zloh, M., and Gibbons, S. (2005) Isopimaric acid from Pinus nigra shows activity against multidrug-resistant and EMRSA strains of Staphylococcus aureus. Phytotherapy Research, 19:538–542.CrossRefGoogle ScholarPubMed
Stanley, D., Keyburn, A.L., Denman, S.E., and Moore, R.J. (2012) Changes in the caecal microflora of chickens following Clostridium perfringens challenge to induce necrotic enteritis. Veterinary Microbiology, 159:155–162.CrossRefGoogle ScholarPubMed
Tansuphasiri, U. (2001) Development of duplex PCR assay for rapid detection of enterotoxigenic isolates of Clostridium perfringens. Southeast Asian Journal of Tropical Medicine and Public Health, 32:105–113.Google ScholarPubMed
Timbermont, L., Haesebrouck, F., Ducatelle, R., and Van Immerseel, F. (2011) Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian Pathology, 40:341–317.Google Scholar
Van der Hoeven Hangoor, E., van der Vossen, J.M., Schuren, F.H., Verstegen, M.W., de Oliveira, J.E., Montijn, R.C., and Hendriks, W.H. (2013) Ileal microbiota composition of broilers fed various commercial diet compositions. Poultry Science, 92:2713–2723.Google Scholar
Van Immerseel, F., De Buck, F.J., Pasmans, F., Huyghebaert, G., Haesebrouck, F., and Ducatelle, R. (2004) Clostridium perfringens in poultry: an emerging threat for animal and public health. Avian Pathology, 33:537–549.Google Scholar
Van Immerseel, F., Rood, J.I., Moore, R.J., and Titball, R.W. (2009) Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens. Trends in Microbiology, 17:32–36.Google Scholar
Wegener, H. (2003) Antibiotics in animal feeds and their role in resistance development. Current Opinions in Microbiology, 6:439–445.Google Scholar
Wienemann, T., Schmitt-Wagner, D., Meuser, K., Segelbacher, G., Schink, B., Brune, A., and Berthold, P. (2011) The bacterial microbiota in the ceca of Capercaillie (Tetrao urogallus) differs between wild and captive birds. Systematic and Applied Microbiology, 34:542–551.Google Scholar
Witte, W., Klare, I., and Werner, G. (1999) Selective pressure by antibiotics as feed additives. Infection, 27, Suppl 2:S35–38.Google Scholar