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Determination of minimum inhibitory and minimum bactericidal concentrations of Brazilian strains of Leptospira spp. for streptomycin sulphate

Published online by Cambridge University Press:  25 September 2020

Bruno Ribeiro Rocha ,
Gabriel Mendes de Souza Martins ,
Breno Cayeiro Cruz  and
Walter Lilenbaum Open the ORCID record for Walter Lilenbaum [Opens in a new window]
Bruno Ribeiro Rocha
Affiliation:
Universidade Federal Fluminense, Niterói, RJ, Brazil
Gabriel Mendes de Souza Martins
Affiliation:
Universidade Federal Fluminense, Niterói, RJ, Brazil Faculdade de Medicina de Petrópolis/Faculdade Arthur Sá Earp Neto, Petrópolis, RJ, Brazil
Breno Cayeiro Cruz
Affiliation:
Ourofino Saúde Animal Ltda, Ribeirão Preto, Brazil
Walter Lilenbaum*
Affiliation:
Universidade Federal Fluminense, Niterói, RJ, Brazil
*
Author for correspondence: Walter Lilenbaum, E-mail: wlilenbaum@id.uff.br
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Type
Commentary
Information
Epidemiology & Infection , Volume 148 , 2020 , e235
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Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

To the Editor,

In 2018, our group published a paper reporting on the reduced antimicrobial susceptibility of Leptospira strains of animal origin in this prestigious journal [Reference Correia, Loureiro and Lilenbaum1]. Meanwhile, new strains were characterised, enlarging the epidemiological profile of leptospirosis in our scenario. We followed the same methodology of the previous paper and tested them against streptomycin sulphate. In this context, the goal of this letter is to enlarge and update the knowledge about the susceptibility of leptospiral strains against streptomycin sulphate.

The current study determined the minimum inhibitory and bactericidal concentrations (MIC and MBC) of streptomycin sulphate, in comparison with six local strains of serogroups Sejroe, Icterohaemorrhagiae, Grippotyphosa and Pomona, belonging to species Leptospira interrogans, L. santarosai and L. kirschneri (Table 1). Strains were maintained in liquid nitrogen, belonging to the Bacteria Collection of Veterinary Interest of the Universidade Federal Fluminense, Rio de Janeiro, Brazil (http://labv.uff.br/ccbvet/#).

Table 1.

Minimum inhibitory and bactericidal concentrations (MIC and MBC) of streptomycin sulphate in six selected local strains of Leptospira

In this study, MIC values of streptomycin across the different Leptospira strains ranged from 0.39 to 3.13 μg/ml, with MBCs ranging from 1.56 to 25 μg/ml (Table 1). These results showed a wide variation in the susceptibility of the strains against streptomycin, not only regarding MIC, but also MBC. The obtained outcomes are consistent with the values observed in other studies such as Correia et al. [Reference Correia, Loureiro and Lilenbaum1] and Liegeon et al. [Reference Liegeon, Delory and Picardeau2], and could define all studied strains as sensitive to streptomycin. These findings validate the usage of streptomycin in field conditions, a fact that can be considered positive, since this is the most common antibiotic used for the treatment of leptospirosis in livestock, particularly ruminants.

Data availability statement

Data are available on request from the authors.

References

Correia, L, Loureiro, AP and Lilenbaum, W (2018) Reduced susceptibility in leptospiral strains of bovine origin might impair antibiotic therapy. Epidemiology and Infection 147, 1–6.Google Scholar
Liegeon, G, Delory, and Picardeau, M (2018) Antibiotic susceptibilities of livestock isolates of Leptospira. International Journal of Antimicrobial Agents 51, 693–699.CrossRefGoogle ScholarPubMed
Figure 0

Table 1. Minimum inhibitory and bactericidal concentrations (MIC and MBC) of streptomycin sulphate in six selected local strains of Leptospira