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Identification of alleles and genotypes of beta-casein with DNA sequencing analysis in Chinese Holstein cow

Published online by Cambridge University Press:  07 September 2016

Ronghua Dai ,
Yu Fang ,
Wenjing Zhao ,
Shuyun Liu ,
Jinmei Ding ,
Ke Xu ,
Lingyu Yang ,
Chuan He ,
Fangmei Ding  and
He Meng
Ronghua Dai
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Yu Fang
Affiliation:
Shanghai Personal Biotechnology Co., Ltd., Shanghai 200240, P. R. China
Wenjing Zhao
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Shuyun Liu
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Jinmei Ding
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Ke Xu
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Lingyu Yang
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Chuan He
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Fangmei Ding
Affiliation:
Shanghai Personal Biotechnology Co., Ltd., Shanghai 200240, P. R. China
He Meng*
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
*
*For correspondence; e-mail: menghe@sjtu.edu.cn
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Abstract

The study reported in this Regional Research Communication aimed to analyse the genetic polymorphisms of β-casein in Chinese Holstein cows. β-casein has received considerable research interest in the dairy industry and animal breeding in recent years as a source not only of high quality protein, but also of bioactive peptides that may be linked to health effects. Morever, the polymorphic nature of β-casein and its association with milk production traits, composition, and quality also attracted several efforts in evaluating the allelic distribution of β-casein locus as a potential dairy trait marker. However, few data on beta-casein variants are available for the Chinese Holstein cow. In the present paper, one hundred and thirty three Holstein cows were included in the analysis. Results revealed the presence of 5 variants (A1, A2, A3, B and I), preponderance of the genotype A1A2 (0·353) and superiorities of A1/A2 alleles (0·432 and 0·459, respectively) in the population. Sequence analysis of β-casein gene in the cows showed four nucleotide changes in exon 7. Our study can provide reference and guidance for selection for superior milk for industrial applications and crossbreeding and genetic improvement programmes.

Keywords

Holstein cowβ-caseinAllele frequencyDNA sequencing
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 312 - 316
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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