Hostname: page-component-8448b6f56d-tj2md Total loading time: 0 Render date: 2024-04-18T12:40:27.969Z Has data issue: false hasContentIssue false
  • English
  • Français

Regeneration of the tympanic membrane using fibroblast growth factor-2

Published online by Cambridge University Press:  18 July 2018

Z-C Lou ,
Z-H Lou  and
J Xiao
Z-C Lou*
Affiliation:
Department of Otorhinolaryngology, Affiliated YiWu Hospital of Wenzhou Medical University, Zhejiang, China
Z-H Lou
Affiliation:
Department of Clinical Medicine, Xinxiang Medical University, Xinxiang City, China
J Xiao
Affiliation:
Molecular Pharmacology Research Center, School of Pharmacy, Zhejiang Provincial Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Zhejiang, China
*
Author for correspondence: Dr Zheng-Cai Lou, Department of Otorhinolaryngology, Affiliated YiWu Hospital of Wenzhou Medical University, 699 Jiangdong Road, YiWu, Zhejiang 322000, China Fax: +86 0579 520 9678 E-mail: louzhengcai@163.com.
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

A systematic review was conducted to investigate the effectiveness of fibroblast growth factor-2 on the regeneration of tympanic membrane perforation.

Methods

The PubMed database was searched for relevant studies. Experimental studies, human randomised controlled trials, prospective single-arm studies and retrospective studies reporting acute and chronic tympanic membrane perforations in relation to two healing outcomes (success rate and closure time), were selected.

Results

All 11 clinical studies investigating the effect of fibroblast growth factor-2 on traumatic tympanic membrane perforations in humans reported a success rate of 89.3–100 per cent, with a closure time of around 2 weeks. Three studies of fibroblast growth factor-2 combined with Gelfoam showed that the success rate of chronic tympanic membrane perforation was 83–98.1 per cent in the fibroblast growth factor-2 group, but 10 per cent in the gelatine sponge groups.

Conclusion

Fibroblast growth factor-2 with or without biological material patching promotes regeneration in cases of acute and chronic tympanic membrane perforation, and is safe and efficient. However, the best dosage, application time and administration pathway of fibroblast growth factor-2 are still to be elucidated.

Keywords

Tympanic MembraneRegenerationFibroblast Growth Factor 2
Type
Review Article
Information
The Journal of Laryngology & Otology , Volume 132 , Issue 6 , June 2018 , pp. 470 - 478
Copyright
Copyright © JLO (1984) Limited, 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Dr Z-C Lou takes responsibility for the integrity of the content of the paper

References

1Buntrock, P, Buntrock, M, Marx, I, Kranz, D, Jentzsch, KD, Heder, G. Stimulation of wound healing, using brain extract with fibroblast growth factor (FGF) activity. III. Electron microscopy, autoradiography, and ultrastructural autoradiography of granulation tissue. Exp Pathol 1984;26:247–54Google ScholarPubMed
2Davidson, JM, Klagsbrun, M, Hill, KE, Buckley, A, Sullivan, R, Brewer, PS et al. Accelerated wound repair, cell proliferation, and collagen accumulation are produced by a cartilage-derived growth factor. J Cell Biol 1985;100:1219–27CrossRefGoogle ScholarPubMed
3Baird, A, Walicke, PA. Fibroblast growth factors. Br Med Bull 1989;45:438–52Google Scholar
4Mondain, M, Saffiedine, S, Uziel, A. Fibroblast growth factor improves the healing of experimental tympanic membrane perforations. Acta Otolaryngol 1991;111:337–41Google Scholar
5Fina, M, Bresnick, S, Baird, A, Ryan, A. Improved healing of tympanic membrane perforations with basic fibroblast growth factor. Growth Factors 1991;5:265–72CrossRefGoogle ScholarPubMed
6Fina, M, Baird, A, Ryan, A. Direct application of basic fibroblast growth factor improves tympanic membrane perforation healing. Laryngoscope 1993;103:804–9CrossRefGoogle ScholarPubMed
7Vrabec, JT, Schwaber, MK, Davidson, JM, Clymer, MA. Evaluation of basic fibroblast growth factor in tympanic membrane repair. Laryngoscope 1994;104:1059–64Google Scholar
8Chauvin, K, Bratton, C, Parkins, C. Healing large tympanic membrane perforations using hyaluronic acid, basic fibroblast growth factor, and epidermal growth factor. Otolaryngol Head Neck Surg 1999;121:43–7Google Scholar
9Hakuba, N, Tabata, Y, Hato, N, Fujiwara, T, Gyo, K. Gelatin hydrogel with basic fibroblast growth factor for tympanic membrane regeneration. Otol Neurotol 2014;35:540–4Google Scholar
10Zhang, D, Huang, Z, Sun, P, Huang, H, Zhang, Y, Dai, J et al. Acceleration of healing of traumatic tympanic membrane perforation in rats by implanted collagen membrane integrated with collagen-binding basic fibroblast growth factor. Tissue Eng Part A 2017;23:20–9CrossRefGoogle ScholarPubMed
11Kato, M, Jackler, RK. Repair of chronic tympanic membrane perforations with fibroblast growth factor. Otolaryngol Head Neck Surg 1996;115:538–47Google Scholar
12Ozkaptan, Y, Gerek, M, Simşek, S, Deveci, S. Effects of fibroblast growth factor on the healing process of tympanic membrane perforations in an animal model. Eur Arch Otorhinolaryngol 1997;254:S2–5Google Scholar
13Santa Maria, PL, Weierich, K, Kim, S, Yang, YP. Heparin binding epidermal growth factor-like growth factor heals chronic tympanic membrane perforations with advantage over fibroblast growth factor 2 and epidermal growth factor in an animal model. Otol Neurotol 2015;36:1279–83Google Scholar
14Lou, Z. Healing large traumatic eardrum perforations in humans using fibroblast growth factor applied directly or via gelfoam. Otol Neurotol 2012;33:1553–7Google Scholar
15Lou, Z, Tang, Y, Wu, X. Analysis of the effectiveness of basic fibroblast growth factor treatment on traumatic perforation of the tympanic membrane at different time points. Am J Otolaryngol 2012;33:244–9Google Scholar
16Zhang, Q, Lou, Z. Impact of basic fibroblast growth factor on healing of tympanic membrane perforations due to direct penetrating trauma: a prospective non-blinded/controlled study. Clin Otolaryngol 2012;37:446–51Google Scholar
17Lou, ZC, Wang, YB. Healing outcomes of large (>50%) traumatic membrane perforations with inverted edges following no intervention, edge approximation and fibroblast growth factor application; a sequential allocation, three-armed trial. Clin Otolaryngol 2013;38:289–96Google Scholar
18Lou, Z, Wang, Y, Yu, G. Effects of basic fibroblast growth factor dose on traumatic tympanic membrane perforation. Growth Factors 2014;32:150–4Google Scholar
19Lou, Z, Wang, Y. Evaluation of the optimum time for direct application of fibroblast growth factor to human traumatic tympanic membrane perforations. Growth Factors 2015;33:65–70Google Scholar
20Lou, Z, Lou, Z, Tang, Y, Xiao, J. Utility of basic fibroblast growth factor in the repair of blast-induced total or near-total tympanic membrane perforations: a pilot study. Am J Otolaryngol 2015;36:794–7CrossRefGoogle ScholarPubMed
21Lou, Z, Yang, J, Tang, Y, Xiao, J. Risk factors affecting human traumatic tympanic membrane perforation regeneration therapy using fibroblast growth factor-2. Growth Factors 2015;33:410–18Google Scholar
22Lou, Z, Huang, P, Yang, J, Xiao, J, Chang, J. Direct application of bFGF without edge trimming on human subacute tympanic membrane perforation. Am J Otolaryngol 2016;37:156–61Google Scholar
23Zhengcai-Lou, , Zihan-Lou, , Yongmei-Tang, . Comparative study on the effects of EGF and bFGF on the healing of human large traumatic perforations of the tympanic membrane. Laryngoscope 2016;126:E23–8Google Scholar
24Lou, ZC, Lou, ZH, Liu, YC, Chang, J. Healing human moderate and large traumatic tympanic membrane perforations using basic fibroblast growth factor, 0.3% ofloxacin eardrops, and Gelfoam patching. Otol Neurotol 2016;37:735–41Google Scholar
25Kanemaru, S, Umeda, H, Kitani, Y, Nakamura, T, Hirano, S, Ito, J. Regenerative treatment for tympanic membrane perforation. Otol Neurotol 2011;32:1218–23CrossRefGoogle ScholarPubMed
26Acharya, AN, Coates, H, Tavora-Vièira, D, Rajan, GP. A pilot study investigating basic fibroblast growth factor for the repair of chronic tympanic membrane perforations in pediatric patients. Int J Pediatr Otorhinolaryngol 2015;79:332–5Google Scholar
27Omae, K, Kanemaru, SI, Nakatani, E, Kaneda, H, Nishimura, T, Tona, R et al. Regenerative treatment for tympanic membrane perforation using gelatin sponge with basic fibroblast growth factor. Auris Nasus Larynx 2017;44:664–71Google Scholar
28Hakuba, N, Taniguchi, M, Shimizu, Y, Sugimoto, A, Shinomori, Y, Gyo, K. A new method for closing tympanic membrane perforations using basic fibroblast growth factor. Laryngoscope 2003;113:1352–5Google Scholar
29Hakuba, N, Iwanaga, M, Tanaka, S, Hiratsuka, Y, Kumabe, Y, Konishi, M et al. Basic fibroblast growth factor combined with atelocollagen for closing chronic tympanic membrane perforations in 87 patients. Otol Neurotol 2010;31:118–21Google Scholar
30Hakuba, N, Hato, N, Omotehara, Y, Okada, M, Gyo, K. Epithelial pearl formation following tympanic membrane regeneration therapy using an atelocollagen/silicone membrane and basic fibroblast growth factor: our experience from a retrospective study of one hundred sixteen patients. Clin Otolaryngol 2013;38:394–7Google Scholar
31Hakuba, N, Ikemune, K, Okada, M, Hato, N. Use of ambulatory anesthesia with manually assisted ventilation for tympanic membrane regeneration therapy in children. Am J Otolaryngol 2015;36:153–7Google Scholar
32Hakuba, N, Hato, N, Okada, M, Mise, K, Gyo, K. Preoperative factors affecting tympanic membrane regeneration therapy using an atelocollagen and basic fibroblast growth factor. JAMA Otolaryngol Head Neck Surg 2015;141:60–6Google Scholar
33Burgess, WH, Maciag, T. The heparin-binding (fibroblast) growth factor family of proteins. Annu Rev Biochem 1989;58:575–606Google Scholar
34Bashkin, P, Doctrow, S, Klagsbrun, M, Svahn, CM, Folkman, J, Vlodavsky, I. Basic fibroblast growth factor binds to subendothelial extracellular matrix and is released by heparitinase and heparin-like molecules. Biochemistry 1989;28:1737–43CrossRefGoogle ScholarPubMed
35DeBlois, C, Cote, MF, Doillon, CJ. Heparin-fibroblast growth factor-fibrin complex: in vitro and in vivo applications to collagen-based materials. Biomaterials 1994;15:665–72Google Scholar
36Baird, A. Potential mechanisms regulating the extracellular activities of basic fibroblast growth factor (FGF-2). Mol Reprod Dev 1994;39:43–8Google Scholar
37Mondain, M, Ryan, A. Histological study of the healing of traumatic tympanic membrane perforation after basic fibroblast growth factor application. Laryngoscope 1993;103:312–18Google Scholar
38Mondain, M, Ryan, A. Effect of basic fibroblast growth factor on normal tympanic membrane. Am J Otolaryngol 1994;15:344–50Google Scholar
39Ishimoto, S, Ishibashi, T. Induction of growth factor expression is reduced during healing of tympanic membrane perforations in glucocorticoid-treated rats. Ann Otol Rhinol Laryngol 2002;111:947–53CrossRefGoogle ScholarPubMed
40Ishimoto, S, Ishibashi, T, Bottaro, DP, Kaga, K. Direct application of keratinocyte growth factor, basic fibroblast growth factor and transforming growth factor-alpha during healing of tympanic membrane perforation in glucocorticoid-treated rats. Acta Otolaryngol 2002;122:468–73Google Scholar
41Niklasson, A, Tano, K. The Gelfoam® plug: an alternative treatment for small eardrum perforations. Laryngoscope 2011;121:782–4Google Scholar
42Tamae, A, Komune, S. Clinical study of transcanal closure of tympanic membrane perforations using a collagen sponge. J Laryngol Otol 2015;129:S21–6Google Scholar
43Bennett, NT, Schultz, GS. Growth factors and wound healing: part II. Role in normal and chronic wound healing. Am J Surg 1993;166:74–81Google Scholar
44Lynch, SE, Colvin, RB, Antoniades, HN. Growth factors in wound healing. Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest 1989;84:640–6Google Scholar
45Rifkin, DB, Moscatelli, D. Recent developments in the cell biology of basic fibroblast growth factor. J Cell Biol 1989;109:1–6Google Scholar
46Goldman, SA, Siegfried, J, Scoleri, P, Aydogen, LB, Cass, SP. The effect of acidic fibroblast growth factor and live yeast cell derivative on tympanic membrane regeneration in a rat model. Otolaryngol Head Neck Surg 1997;117:616–21Google Scholar
47Kälicke, T, Köller, M, Frangen, TM, Schlegel, U, Sprutacz, O, Printzen, G et al. Local application of basic fibroblast growth factor increases the risk of local infection after trauma: an in vitro and in vivo study in rats. Acta Orthop 2007;78:63–73Google Scholar
48Ishibashi, T, Shinogami, M, Ishimoto, SI, Yoshida, K, Kaga, K. Induction of KGF, basic FGF, and TGFalpha mRNA expression during healing of experimental TM perforations. Acta Otolaryngol 1998;118:701–4Google Scholar
49Santa Maria, PL, Redmond, SL, Atlas, MD, Ghassemifar, R. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope 2010;120:2061–70Google Scholar
50Stenberg, BD, Phillips, LG, Hokanson, JA, Heggers, JP, Robson, MC. Effect of bFGF on the inhibition of contraction caused by bacteria. J Surg Res 1991;50:47–50Google Scholar
51Hayward, P, Hokanson, J, Heggers, J, Fiddes, J, Klingbeil, C, Goeger, M et al. Fibroblast growth factor reserves the bacterial retardation of wound contraction. Am J Surg 1992;163:288–93Google Scholar
52Kase, K, Iwanaga, T, Terakado, M, Tanaka, F, Takasaki, K, Kumagami, H et al. Influence of topical application of basic fibroblast growth factor upon inner ear. Otolaryngol Head Neck Surg 2008;138:523–7Google Scholar
53Zhai, SQ, Wang, DJ, Wang, JL, Han, DY, Yang, WY. Basic fibroblast growth factor protects auditory neurons and hair cells from glutamate neurotoxicity and noise exposure. Acta Otolaryngol 2004;124:124–9Google Scholar
54Low, W, Dazert, S, Baird, A, Ryan, AF. Basic fibroblast growth factor (FGF-2) protects rat cochlear hair cells in organotypical culture from aminoglycoside injury. J Cell Physiol 1996;167:443–50Google Scholar
55Sekiya, T, Shimamura, N, Yagihashi, A, Suzuki, S. Effect of topically applied basic fibroblast growth factor on injured cochlear nerve. Neurosurgery 2003;52:900–7CrossRefGoogle ScholarPubMed
56Friedman, NR, Wright, CG, Pawlowski, KS, Meyerhoff, WL. Effect of basic fibroblast growth factor on perforated chinchilla tympanic membranes. Ear Nose Throat J 1997;76:559–64Google Scholar
57Hennessey, PJ, Nirgiotis, JG, Shinn, MN, Andrassy, RJ. Continuous EGF application impairs long-term collagen accumulation during wound healing in rats. J Pediatr Surg 1991;26:362–5Google Scholar