Introduction
The Golden-cheeked Warbler Dendroica chrysoparia is a ‘Critically Endangered’ Nearctic-Neotropical migratory bird that breeds in Texas and winters from Chiapas, Mexico, to north-west Nicaragua. Past work has indicated that wintering Golden-cheeked Warblers are associated with pine-oak forests (Vidal et al. Reference Vidal, Macias-Caballero and Duncan1994, Rappole et al. Reference Rappole, King and Leimgruber2000, Rappole et al. Reference Rappole, King and Diez2003). The pine-oak forests that are inhabited by wintering Golden-cheeked Warblers are considered critically endangered due to their restricted geographic distribution and threats from expanding human activity (Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008). An understanding of the association between Golden-cheeked Warbler abundance and habitat characteristics is important for developing strategies for the conservation of this species (Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008).
Studies of wintering Golden-cheeked Warblers have shown that warblers forage preferentially on the leaves of oaks such as Quercus sapotifolia, Q. eliptica and Q. elongata, which have shiny, narrow, elliptical, or oblong leaves, and are collectively referred to as ‘encino’ oaks in our Honduran study areas (Rappole et al. Reference Rappole, King and Barrow1999). In contrast, Golden-cheeked Warblers forage less than expected in ‘roble’ oaks such as Q. segoviensis, Q. purulhana and Q. rugosa, which have large, lobed leaves. Data collected on transect counts from 1996 to 1998 indicated that points where Golden-cheeked Warblers were detected had more encino oaks than corresponding random locations (Rappole et al. Reference Rappole, King and Barrow1999). Findings such as these have led to the identification of encino as a “critical habitat feature” (sensu Villard and Jonsson Reference Villard, Jonsson, Villard and Jonsson2009a) for non-breeding wintering Golden-cheeked Warbler habitat (Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008).
Although the identification of critical habitat features is valuable for making coarse-filter assessments, modelling variation in abundance over a range of habitat conditions yields information useful for the development of quantitative targets for conservation (Guenette and Villard Reference Guenette and Villard2005). Quantitative targets are important in the context of sustainable forest management for defining desired future conditions needed to support specific biodiversity values (Villard and Jonsson Reference Villard and Jonsson2009b). In the case of the Golden-cheeked Warbler, managers are aware that encino oaks represent a critical habitat feature; however forestry operations are carried out under management plans that prescribe desired future conditions, and in the absence of quantitative targets for the retention of encino oaks for Golden-cheeked Warblers, the success and defensibility of forestry operations in terms of meeting their statutory obligations to conserve endangered species is in doubt. In this study, we analyse abundance of Golden-cheeked Warblers in relation to habitat conditions to aid in the identification of quantitative habitat targets for the management of non-breeding Golden-cheeked Warblers.
Methods
To establish quantitative habitat targets for Golden-cheeked Warblers, we used data collected by Rappole et al. (Reference Rappole, King and Diez2003) from a 30,000 km2 region in the central and western highlands of Honduras. Golden-cheeked Warblers were surveyed on 44 transects located throughout this region in potential Golden-cheeked Warbler habitat, which consists of montane pine-oak forest > 1,000 m above sea level (Rappole et al. Reference Rappole, King and Leimgruber2000). The area surveyed was 74.8% forested, with the remainder consisting of savannah, scrubland and agricultural and developed land (Rappole et al. Reference Rappole, King and Leimgruber2000). Transects averaged 1 km in length and, where possible, were located in a stratified-random manner by choosing a random distance 0–100 m as a starting point within a randomly selected 1-km2 block located on or near a point of ready access (Rappole et al. Reference Rappole, King and Diez2003). Fifteen transects were surveyed in 1996, 25 in 1997, and 4 in 1998. Transects were surveyed by walking slowly (≈ 1 km h–1) and watching and listening for Golden-cheeked Warblers or vocal members of mixed-species flocks with which they associate. When a flock was located, the observer stayed with it until either a Golden-cheeked Warbler had been sighted or the observer determined that it was unlikely that a Golden-cheeked Warbler accompanied the flock. Afterwards, observers measured the distance from the point on the transect from which the flock was located to the point where the first bird in the flock was detected, as well as the angle between the transect and the point where the first bird in the flock was detected in degrees. The average radius of flocks in the study area, 25 m, was added to the distance measurement for analyses (Rappole et al. Reference Rappole, King and Diez2003).
Rappole et al. (Reference Rappole, King and Diez2003) measured habitat characteristics on five randomly located 0.04-ha plots on each transect (James and Shugart Reference James and Shugart1970). These plots were situated by proceeding to points 100, 300, 500, 700 and 900 m along each transect, and from these points, choosing a direction right or left from the transect with a coin toss and selecting the plot location at a random distance 12–100 m from the transect selected with a random number table. Four 11.3-m transects were established radiating from the center of each plot in the cardinal directions. Canopy and herb cover were measured at five points along each transect (20 of each per plot) with a sighting tube, and canopy and herb cover were calculated as the proportion of the 20 points at which canopy and herb cover were sighted at crosshairs fitted to the distal end of the sighting tube (James and Shugart Reference James and Shugart1970). The diameter of all trees on the plots was measured at breast height, and the number of trees of each species and size class (small 1–9 cm, medium > 9–27 cm, and large > 27 cm) within 11.3 m of the centre point was recorded. Habitat variables were averaged for each transect and the averages used in the analyses as possible predictors of Golden-cheeked Warbler abundance.
These data were originally used to calculate population density of wintering Golden-cheeked Warblers (Rappole et al. Reference Rappole, King and Diez2003) using the program DISTANCE (Buckland et al. Reference Buckland, Anderson, Burnham, Laake, Borchers and Thomas2001), and also to identify habitat variables that were associated with Golden-cheeked Warbler presence (Rappole et al. Reference Rappole, King and Barrow1999, Reference Rappole, King and Leimgruber2000). These researchers were unable to directly relate warbler density to habitat characteristics because DISTANCE estimates the effect of habitat covariates on detectability, not abundance. A recently developed technique, however, allows for modelling of both density and detection probability simultaneously (Royle et al. Reference Royle, Dawson and Bates2004). This model is based on specifying the distance-sampling likelihood at each sample unit in terms of abundance with a regression model parameterised in terms of habitat covariates. Maximum-likelihood estimation of detection and density parameters is based on the integrated likelihood. We applied this methodology to the data collected by Rappole et al. (Reference Rappole, King and Diez2003) with the program R (version 2.9.0, R Development Core Team 2009) and the package “unmarked” (version 2.9-0; Fiske and Chandler 2011).
Independent variables included basal area and density of trees (all sizes combined as well as separately by size class) for tree species comprising > 5% of the average basal area, which included pines (mostly Pinus oocarpa and P. maximinoi), encino oaks and roble oaks. These species comprised 93% of the tree basal area. Other variables included percentage tree canopy cover and percentage ground cover. Variables were log or arcsine transformed as appropriate to improve normality, examined for correlation with other predictor variables, and analysed individually as predictors of Golden-cheeked Warbler abundance. Variables that performed better (i.e. lower AICc values) than the intercept only model were retained and included additively in multivariate models. The appropriate abundance model (Poisson versus negative binomial) and detection function (uniform, hazard, half-normal, exponential) was chosen based upon AICc values (Buckland et al. Reference Buckland, Anderson, Burnham, Laake, Borchers and Thomas2001). We examined univariate scatterplots of all habitat variables and fitted non-linear curves (quadratic and sigmoid) where higher order relationships were indicated. Observer was not included as a covariate in the detectability term because the same observer (DK) conducted all of the surveys at these sites. A total of 18 models were run. Models were ranked using AICc values. Those that performed better than the intercept only model are presented, and those with AICc values within 2 units of the highest ranked model were considered to be supported. We encountered substantial model-selection uncertainty and used model averaging to incorporate this uncertainty into parameter estimates (Burnham and Anderson Reference Buckland, Anderson, Burnham, Laake, Borchers and Thomas2002). Goodness of fit was evaluated using the parametric bootstrap procedure based on a chi-squared fit statistic (Royle et al. Reference Royle, Dawson and Bates2004).
Results
During the course of the study we detected 31 Golden-cheeked Warblers on 20 of the 44 transects surveyed. Three individuals were detected on two transects, two individuals on seven transects, and a single individual was sighted on 11 transects. Four models of Golden-cheeked Warbler abundance as a function of habitat variables had ∆AICc values of ≤ 2, and were considered to be supported (Table 1). Because the model weights were distributed relatively evenly among these four models, model averaging was used to incorporate model selection uncertainty into the parameter estimates. The two most supported models had weights that indicated the probability of their being the best model was > twice that of the two other supported models. These two most supported models both included linear and quadratic terms for encino basal area, and the top model included a linear and quadratic term for roble density as well. The top model indicated that the density of Golden-cheeked Warblers increased with encino basal area to ≈ 5.6 m2 ha–1 and with roble density to ≈ 7 trees ha–1, and then decreased thereafter (Figure 1). Bootstrap estimates for 95% confidence intervals of these estimates for encino basal area and roble density were 3.9–6.8 m2 ha–1 and 2.5–20 trees ha–1, respectively. The other supported models included a single term indicating a linear increase of Golden-cheeked Warbler abundance with increasing density of small (1–9 cm dbh) encino oaks and density of all encino size classes combined, respectively. Parametric bootstrap procedures indicated no evidence of over-dispersion for the top-ranked model (P = 0.66) and examination of the model residuals did not indicate the presence of outliers or points with very strong leverage. Neither first order, quadratic nor sigmoid relationships with any other habitat variables (e.g. density of encino, basal area of roble, density or basal area of pine, canopy or ground cover) were included in supported models (∆AICc values of ≤ 2). There was no support for basal area or density of small, medium or large pines or roble oaks or medium or large Encino oaks. Average values for habitat parameters with standard errors are in Table 2.
Relationship between density of Golden-cheeked Warblers and basal area (m2 ha–1) of (a) encino oaks and (b) density of roble oaks (trees ha-1) from data collected on 44 transects in Honduras, Guatemala and Chiapas, Mexico 1996–1998. Response curves represent model-averaged estimates and standard errors.
Model selection results of relationship between Golden-cheeked Warbler density and habitat variables from 44 l-km transects in 1996, 1997, and 1998 in montane pine forests throughout western and central Honduras. Models that performed better than the intercept only model are presented.
1EBA = basal area of encino oaks (m2 ha–1); RD = density (trees ha–1) roble oaks; ED small = density of encino oaks 1–9 cm dbh; ED = density of encino oaks; RBA = basal area of roble oaks, EBA small = basal area encino oaks 1–9 cm dbh.
Mean (SE) values for habitat variables from 44 1-km transects surveyed for Golden-cheeked Warblers in 1996, 1997 and 1998 in montane pine forests throughout western and central Honduras.
a. m2 ha–1
b. trees ha–1
Discussion
The identification of critical habitat features through comparisons of habitat conditions between occupied and unoccupied sites is thought to aid in conservation efforts by providing general criteria for discriminating between suitable and unsuitable habitat for the purpose of habitat inventory or assessments, but there are concerns that this information falls short of that required for integrating biodiversity values into management plans in montane pine-oak forests because forest conditions and species responses are not necessarily dichotomous (Guenette and Villard Reference Guenette and Villard2005). Previous analyses of these data indicated that Golden-cheeked Warblers were detected more often in pine-oak forest (Rappole et al. Reference Rappole, King and Leimgruber2000), and at points that had greater basal area of encino oak than corresponding random points (Rappole et al. Reference Rappole, King and Barrow1999), results which have also been reported from studies in Mexico (Vidal et al. Reference Vidal, Macias-Caballero and Duncan1994) and Nicaragua (King et al. Reference King, Herrera, Hernandez, Lively, Mehlman, Rappole, Roth, Rich, Arizmendi, Demarest and Thompson2009). Habitats with at least as much basal area as these published values measured at occupied sites are probably suitable, and habitats with no encino oaks are clearly unsuitable. However, by treating habitat suitability as a discrete function, conservationists run the risk of mischaracterising the response of species to habitat conditions with potentially important consequences (Guenette and Villard Reference Guenette and Villard2005).
Our analyses of Golden-cheeked Warbler density along a continuous habitat gradient better reflect the actual response of this species to habitat conditions, and permit the establishment of quantitative conservation targets that will enable the accurate identification of suitable habitat to inform conservation efforts. The montane pine-oak forests preferred by the Golden-cheeked Warbler also have substantial commercial and subsistence value (pines primarily for building supplies and oaks for fuel), and much of the region within the winter range of the Golden-cheeked Warbler is lacking in economic activity that could be supplied by the sustainable management of montane pine-oak forests (Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008). An important aspect of the development of this resource is ensuring the maintenance of biodiversity, and because it is an endangered species and a montane pine-oak obligate, the conservation of the Golden-cheeked Warbler clearly should be a component of this conservation effort. Since the retention of all encino oaks could interfere with the cultivation of pines and the use of oaks as fuel, conservation of this species will require the identification of minimum values for habitat conditions below which this species will be scarce or absent. Our analyses indicate that Golden-cheeked Warbler abundance does not increase at values above ≈ 5.6 m2 ha–1 of encino. Since some of the stands we studied contained as much as 29 m2 ha–1 encino basal area, managers could prescribe the harvest of a considerable amount of encino and still provide suitable habitat for Golden-cheeked Warblers.
Our finding that the abundance of Golden-cheeked Warblers was closely associated with encino oaks can be explained by their foraging behaviour. Rappole et al. (Reference Rappole, King and Barrow1999) reported that most (94%) foraging manoeuvres by Golden-cheeked Warblers at these sites were directed at encinos. It is not clear why Golden-cheeked Warbler abundance declined beyond basal areas > 5.6 m2 ha–1 of encino. Models of Golden-cheeked Warbler abundance that included a sigmoid relationship for encino received far less support (∆AICc = 11.6) compared to the quadratic relationship (∆AICc = 3.76), indicating the shape of the curve is not an artefact of fitting a quadratic relationship. Similarly, an ad hoc model including encino basal area as a covariate of detectability had a higher AICc value than the top-ranked model (∆AICc = 2.55) and was thus not supported, indicating declines in abundance with increased encino basal area did not reflect a decrease in detectability. Rather it appears that Golden-cheeked Warblers actually are most abundant in stands with intermediate levels of encino oak basal area. Chandler and King (2011) reported that non-breeding Golden-winged Warblers Vermivora chrysoptera were most abundant at intermediate levels of canopy closure, and suggested that increased light penetration resulted in increased food resources. Perhaps a similar mechanism is responsible for the association of Golden-cheeked Warblers with intermediate levels of encino basal area.
Golden-cheeked Warblers were seldom observed foraging in roble oaks in our study (Rappole et al. Reference Rappole, King and Barrow1999), so there is no clear explanation for their positive relationship up to ≈ 7 robles ha–1. Although supported, the model including both encino basal area and roble density had nearly the same model weight as the model including encino only, indicating roble actually added little extra explanatory power to the model. Furthermore, roble density ranged up to 235 trees ha–1, so the relationship between Golden-cheeked Warbler density and roble density was negative over nearly the entire range of roble density.
We did not find any significant relationship between Golden-cheeked Warblers and pines, which generally dominated the stands where we worked. Golden-cheeked Warblers seldom forage in pines (Rappole et al. Reference Rappole, King and Barrow1999), however nearly all Golden-cheeked Warblers we encountered (99%) were associated with mixed-species flocks, and many of these flock associates, such as Hermit Warblers Dendroica occidentalis and Olive Warblers Peucedramus taeniatus, are closely associated with pines (King and Rappole Reference King and Rappole2000). King et al. (Reference King, Herrera, Hernandez, Lively, Mehlman, Rappole, Roth, Rich, Arizmendi, Demarest and Thompson2009) found that generally Golden-cheeked Warbler habitat selection in Nicaragua was similar to that in the core of their range, however a small number of Golden-cheeked Warblers in their study were encountered at sites with encinos but no pines. The lack of association between Golden-cheeked Warblers and pines suggests that Golden-cheeked Warblers are relatively flexible with respect to their habitat requirements as long as the sites are forested and contain ≈ 5.6 m2 ha–1 of encino basal area.
Landscape composition is considered important in influencing habitat quality of breeding Golden-cheeked Warblers (Magness et al. Reference Magness, Wilkins and Hejl2006, Peak Reference Peak2007), but it is not clear whether non-breeding Golden-cheeked Warblers are affected by landscape composition. Although the high elevation areas within our study area were extensively forested and thus probably above the threshold levels for fragmentation effects to be manifested, the relatively unfragmented condition of our study sites is not representative of other portions of the species range, such as south-eastern Guatemala (Rappole et al. Reference Rappole, King and Leimgruber2000, Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008) or Nicaragua (King et al. Reference King, Herrera, Hernandez, Lively, Mehlman, Rappole, Roth, Rich, Arizmendi, Demarest and Thompson2009). Thus we advise caution in extending our results to less forested parts of this species’ range.
Recently managers have turned to integrating wildlife values into management plans in Mesoamerican montane pine-oak forests, and because these forests are occupied by endangered Golden-cheeked Warblers, the quantitative habitat targets we have generated for this species will be an important component of these efforts (Perez et al. Reference Perez, Secaira, Macias, Morales and Amezcua2008). Although focal-species analyses are an important component of biodiversity conservation efforts (Villard and Jonsson Reference Villard and Jonsson2009b), species-specific efforts lack generality and should be complemented by coarse-filter modelling exercises to encompass the habitat needs of more species (Lindenmayer et al. Reference Lindell, Cole, Holl and Zawahi2006, Lindell et al. Reference Lindenmayer, Franklin and Fischer2011). Because its endangered status makes monitoring a priority, investigations of the extent to which Golden-cheeked Warblers could indicate habitat quality for other sensitive species would be valuable.
Acknowledgements
We thank the USDA Forest Service International Program who funded this work through a cooperative agreement with The Nature Conservancy Migratory Bird Program. We also thank Edgard Herrera for his valuable advice and support, Martha Moreno, Marvin Martinez, and Julio Merida for their help with fieldwork 2006–2008, and Scott Schlossberg and Mitch Hartley for his comments on an earlier draft of this manuscript.
