Serotype composition of vaccines

PCV-13 contains 13 serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F). Serotype 6C is also covered through cross-protection provided by the 6A component [Reference Feemster11]. PCV15 adds two serotypes (22F and 33F) and PCV-20 adds five non-PCV15 serotypes (8, 10A, 11A, 12F, and 15C). Serotypes 15B and 15C should be considered as a single entity (15B/C) and cross-protective antibodies are generated by each of the two components [Reference Venkateswaran12, Reference Hao13]. PCV-21 was designed to target frequent serotypes in adults in a context of PCV use in children: 11 serotypes also in PCV-20 (3, 6A, 7F, 8, 10A, 11A, 12F, 15C, 19A, 22F, and 33F) and 10 specific serotypes (9 N, 15A, 16F, 17F, 20, 23A, 23B, 24F, 31, and 35B). Together, PCV-20 and PCV-21 cover 31 serotypes (30 vaccine-serotypes + 6C). These two vaccines are highly complementary and their combined use in a specific population would provide optimal broad protection.

Coverage of invasive pneumococcal disease strains

In Canada, the National Microbiology Laboratory (NML) in Winnipeg, Manitoba, affiliated with the Public Health Agency of Canada has the mandate of IPD surveillance [Reference Golden14, Reference Griffith15]. Invasive Sp isolates and biological specimens are transmitted from provincial and territorial public health laboratories for confirmation of bacteriological diagnosis, serotyping, and analysis of the antimicrobial resistance profile. For the province of Quebec, selected culture-negative biological specimens and Sp isolates are transmitted along with aggregated data on all IPD cases reported to the Laboratoire de Santé publique du Québec (LSPQ) affiliated with the Institut national de Santé publique du Québec (INSPQ).

During the period 2018–2022, an important variation in the total number of culture-positive IPD cases was observed (3340 cases in 2018, 3690 in 2019, 2122 in 2020, 2008 in 2021, and 3846 in 2022), a consequence of the many disturbances associated with the COVID-19 pandemic and a surge in respiratory viral infections starting in the fall of 2021 when COVID-19 control measures were lifted [Reference Ouldali16].

The serotype distribution of IPD cases with a culture originating from a normally sterile site or liquid reported in the years 2018–2022 and with information on age is shown in Table 1. The proportion of IPD cases attributable to PCV-13-serotypes was higher in the 5–64 years age group (42%) than in younger and older age groups (24% and 30%, respectively). The two additional serotypes included in PCV-15 accounted for about 11% of cases in all age groups. The fraction of IPD cases attributable to the five additional serotypes included in PCV-20 decreased with age: 25% in children <5 years, 20% in 5 to 64-year-olds, and 15% in elderly adults. In contrast, the proportion of cases caused by the 10 serotypes specific to PCV-21 was 24% in children and middle-aged adults and 33% in elderly adults. During the study years, non-vaccine types (NVT) represented about 7% of all IPD cases.

Table 1.

The proportion of IPD cases reported in Canada in 2018–2022 belonging to Streptococcus pneumoniae serotypes included in 13-valent, 15-valent, 20-valent, and 21-valent pneumococcal conjugate vaccines and the proportion of cases showing resistance to at least one or three antimicrobials

^a Serotypes 15B and 15C are combined into one category.

^b Number of isolates with completed antimicrobial resistance testing is lower than the total submitted to the laboratory.

^c Including ST-6C. NVT = Non-Vaccine-Type.

Trends in the distribution of serotypes were observed in recent years, with an increase in the proportion of serotypes covered by PCV-20 and a decrease in the proportion covered by PCV-21, these two opposite trends being more pronounced in the 5–64 years age groups than in elderly adults and children (not shown in the table). Currently, serotype distribution across age groups suggests that PCV-20 is particularly suited for children, while PCV-21 is the best choice for elderly adults, especially if PCV-20 is used in children, generating a herd effect and serotype replacement, thereby increasing the incidence of PCV-21-specific serotypes in adults.

Coverage of strains in asymptomatic carriers

Asymptomatic carriers play a key role in the transmission of Sp and the epidemiology of IPD [Reference Simell17]. The prevalence of Sp carriage in the population is influenced by age, with children of kindergarten age (2–5 years) having the highest rates [Reference Wang18, Reference Lozada19]. Carriage density of is a key factor in the transmission and is highest in young children [Reference Roca20]. The immunization of children with PCVs has been associated with a reduction in the proportion of carriers of vaccine serotypes and an increase in the proportion of non-vaccine serotypes, the replacement being generally complete or sometimes partial [Reference Fleming-Dutta21].

Information on the dynamic of carriage in Canada is limited [Reference Ricketson22]. In the United Kingdom, two large surveys were conducted in the context of a mature PCV-13 programme based on a 2 + 1 schedule as in Canada [Reference Southern23, Reference Tiley24]. In children 1–4 years of age, the proportion of strains identified in carriers belonging to PCV-15-specific serotypes (22F and 33], were respectively 7% and 6% in the two surveys, the proportion being respectively 36% and 27% for PCV-20-specific serotypes (8, 10A, 11A, 12F, 15B/C, 22F, and 33F) [Reference Southern23, Reference Tiley24]. These observations suggest that the magnitude of replacement could be higher if PCV-20 replaces PCV-13 than if PCV-15 replaces PCV-13. The consequence of this replacement would, however, be determined by the invasiveness and virulence of the serotypes that will occupy an empty ecological niche.

Correlates of protection for invasive pneumococcal disease

PCV-13 serotype-specific correlates of protection for infants have been estimated by linking serotype-specific IgG concentration distributions after two priming doses in randomized clinical trials (RCTs) and serotype-specific vaccine effectiveness (VE) estimates using the indirect cohort method in the UK [Reference Andrews25]. Three serotypes have a correlate of protection well above the 0.35 μg/mL benchmark value: ST-3 (2.83 μg/mL), ST-19F (1.17 μg/mL), and ST-19A (1.00 μg/mL). These three serotypes have been imperfectly controlled by PCV-13 childhood programmes with high uptake rates as shown in Canada [Reference Griffith15]. Correlates of protection against nasopharyngeal infection are also much higher than against IPD: 5 μg/mL on average in a study in the USA and there is also a variation according to the serotype [Reference Voysey26, Reference Pham and Pichichero27]. An increase in the incidence of antibody-resistant serotypes in the overall population cannot thus be excluded if a less immunogenic and effective vaccine replaces PCV-13 in children.

Antimicrobial resistance

Antimicrobial resistance of Sp strains seems to be more associated with clonal genotypes than serotypes [Reference Song28]. For this reason, the proportion of resistant IPD strains within a specific serotype varies with time and geography. At MNL, IPD strains isolated from cultures are tested for resistance to seven drug classes including β-lactams, macrolides, tetracyclines, fluoroquinolones, clindamycin, chloramphenicol, and trimethoprim/sulfamethoxazole. For β-lactams, resistance is tested for penicillin (Clinical and Laboratory Standards Institute oral breakpoint) and ceftriaxone (Clinical and Laboratory Standards Institute meningitis breakpoint). Methods to assess resistance are described in a previous publication [Reference Demczuk29]. The antimicrobial profile of strains belonging to different serotype groups is shown in Table 1. Note that not all strains submitted to NML are tested. Those originating from LSPQ are not included in the Table as they represent a biased sample of isolates from Quebec.

Among the strains covered by PCV-13, 13% are multiresistant, defined as a resistance to three or more antimicrobial classes. Multiresistant strains in this PCV-13 group mostly belong to serotypes 19A and 9 V. Serotypes 22F and 33F, which are covered by PCV-15, PCV-20, and PCV-21 have a high frequency of monoresistance (59%) but a low frequency of multiresistance (3%). PCV-20 covers 5 serotypes not in PCV-15. Among these, serotypes 11A, 12F, and 15B/C have a high frequency of monoresistance, although multiresistance is less common in this group. Among PCV-21-specific serotypes, three are particularly problematic: 15A (53% monoresistance and 43% multiresistance), 23A (37% monoresistance and 24% multiresistance), and 24F (78% monoresistance and 44% multiresistance).

Antimicrobial resistance is one of the plausible mechanisms that favour the emergence of a particular serotype following the introduction of a new PCV in a childhood programme [Reference Weinberger30]. Antibiotic resistance may be problematic at the clinical level for the treatment of otitis media mainly [Reference Xu31]. Using the vaccine with the broadest coverage of resistant serotypes for the childhood programme would be preferable, and PCV-20 has an advantage over PCV-15 in this regard.

Invasiveness

Invasiveness can be defined as the capacity of a particular organism to spread from a primary nasopharyngeal mucosal infection to a systemic or localized secondary infection and can be measured as the number of IPDs per colonization event in a longitudinal study (the attack rate) or as the case-to-carrier ratio combining IPD surveillance and carriage prevalence data [Reference Metcalf32]. Invasiveness is more related to capsular characteristics defining the serotype than clonal genotypes having different capsular expressions [Reference Sleeman33].

Two meta-analyses reporting serotype-specific invasiveness indices have been published [Reference Balsells34, Reference Løchen35]. The first one was based on four studies published between 2000 and 2015, along with 13 unpublished datasets [Reference Balsells34]. Invasiveness was measured as the ratio of IPD incidence to carriage prevalence in children 0–59 months of age in a particular setting using the ST-19A ratio as a reference for the estimation of serotype-specific odds ratios (OR) in a random-effects model. In the other meta-analysis, Bayesian models were applied to 20 datasets in children and five combining data on child carriage and adult IPD [Reference Løchen35]. The advantage of this Bayesian methodology over the traditional OR technique is that it provides absolute rather than relative indices, which are more robust for infrequent serotypes, although there was a good level of concordance between results produced by the two methods [Reference Løchen35]. For our purpose, absolute invasiveness indices in the second meta-analysis were converted into relative indices using the median ST-17F value as a common reference.

As shown in Table 2, ST 22F has a lower than the median invasiveness value and the ST 33F value is close to the median in the first meta-analysis [Reference Balsells34]. The invasiveness ranking of these two serotypes is above average in the second meta-analysis: 18/61 for 22F and 6/61 for 33F [Reference Løchen35]. Among PCV-20-non-PCV-15 serotypes, two are among those having the highest invasiveness indices (ST 8 and ST 12F). These observations are important, as both PCV-15 and PCV-20 are authorized for use in children and may induce a replacement involving with highly invasive serotypes not induced in their composition. The use of PCV-15 could thus open the door to the emergence of ST 8 and/or ST 12F but not if PCV-20 is used. It should be noted that serotypes not included in PCV-20 and having high invasiveness are not many: none in the first meta-analysis, ST-22D (4/61), ST-38 (7/61), and ST-10F (8/61) in the second metanalysis [Reference Balsells34, Reference Løchen35]. This suggests that PCV-20 has the potential to mitigate serotype replacement more effectively than PCV-15, a hypothesis supported by the results of a dynamic model predicting the impact of a shifting from PCV-13 to PCV-15 or PCV-20 for childhood immunization in the United Kingdom [Reference Choi38].

Table 2.

Serotype-specific relative invasiveness and virulence indices

NA: Not available. RI: Relative invasiveness. CFR: Case fatality ratio.

15B/C: ST 15B and ST 15C are considered as a single entity.

It should be stressed that this analysis has several limitations. First, there was inter-study variation in the two meta-analyses. Second, confidence intervals were not considered in our ranking. Finally, the invasiveness indices in the two meta-analyses were based on carriage prevalence data and rather than carriage incidence data, which can only be estimated in a longitudinal study with repeated samplings with short intervals, a rarity. Obviously, there is an association between the acquisition (incidence) rate of carriage and the prevalence rate of carriage, but this relationship is somewhat confounded by carriage duration, which has been shown to vary between serotypes [Reference Sleeman33].

Virulence

The case-fatality ratio (CFR) is the most easily defined and commonly used marker of Sp infection virulence and is a stable serotype-associated property [Reference Weinberger39, Reference Grabenstein and Musey40]. Serotypes with a high CFR tend to exhibit a high carriage prevalence, low invasiveness, and a thicker capsule in vitro [Reference Weinberger39]. Two meta-analyses on serotype-specific CFRs have been published and the most comprehensive and recent one was selected [Reference Demirdal36]. A large case series from Spain covering close to 6000 all-ages IPD cases in 2007–2020 that was not included in the selected meta-analysis was also included in the analysis [Reference De Miguel37].

As shown in Table 2, ST 22F and ST 33F have close to average CFRs. Among the five PCV-20-non-PCV-15 serotypes, ST 11A is particularly virulent, ranking second in both studies. Virulent serotypes are particularly problematic for adults knowing that CFR is increasing with age [Reference Demirdal36]. Interestingly, PCV 21 covers 6 out of the 10 most virulent serotypes identified in the meta-analysis (31, 11A, 35B, 16F, 3, and 17F) and also 6 out of the 10 most virulent serotypes in the Spanish case series (31, 11A, 6C thanks to 6A, 3, 23A, and 9 N) [Reference Demirdal36, Reference De Miguel37]. This means that PCV-21 would be particularly useful for protecting adults against virulent infections.

Our analysis has several limitations. First precision of CFR estimates was not taken into account for ranking and confidence intervals were large for serotypes rarely seen before the PCV-13 introduction [Reference Demirdal36, Reference De Miguel37]. Second, there was some heterogeneity in the ranking as suggested by high I ² values of several summary estimates in the meta-analysis [Reference Demirdal36]. Although imperfect, the ranking presented gives an idea of the serotypes that should be controlled through direct or indirect protection of adults.

Immunogenicity

Pivotal immunogenicity trials in children comparing PCVs were identified in product monographs [2, 3]. Trials were mostly conducted in North America and Western Europe, with measurements of opsonophagocytotic activity geometric mean titres (OPA GMT) selected. The OPA GMT ratio of common serotypes was calculated and the mean of these ratios was interpreted as a proxy of the relative immunogenicity of the vaccines in the comparison [Reference De Wals49]. Results are presented in Table 3. It can be seen that PCV7 was more immunogenic than PCV-13 (2 trials) [Reference Yeh41, Reference Kieninger42], that PCV-13 was more immunogenic than PCV15 (3 trials) [Reference Lupinacci43–Reference Benfield45] and PCV-20 (3 trials) [Reference Senders46–Reference Korbal48].

Table 3.

Summary results of immunogenicity studies comparing pneumococcal conjugate vaccines in children and adults

^a Correction of an error discovered in the Supplementary Table S10 in Martinon-Torres’ manuscript.

There is no head-to-head comparison between PCV-15 and PCV-20 in children. To compare these two vaccines, an indirect comparison was carried out using PCV-13 as a common comparator [Reference De Wals49]. Results suggested that PCV-20 and PCV-15 have a similar immunogenicity profile in both 2 + 1 and 3 + 1 schedules, with OPA ratio values close to unity. In this review of immunogenicity trials, the Phase 2 trial comparing PCV-13 to PCV-21 in a 3 + 1 schedule was included for the indirect comparison [Reference Senders46]. Recently, results of the Phase 3 trial comparing PCV-20 to PCV-13 in a 3 + 1 schedule were published and the performance of PCV-20 was somewhat better with a mean PCV-13:PCV-20 OPA GMT ratio of 0.93 instead of 0.72 in the Phase 2 trial [Reference Senders46, Reference Senders47]. When results of the Phase 3 trial are used for the indirect comparison instead of those of the Phase 2 trial, the average PCV-15:PCV-20 OPA GMT ratio is 0.84, indicating a slightly better performance of PCV-15 compared to PCV-20. However, this has little relevance for Canada, as a 2 + 1 schedule is used for the vast majority of children.

Pivotal immunogenicity trials in adults comparing PCVs with OPA GMT measurements were identified in product monographs and a presentation to the CDC [2, 3, Reference Platt53]. Trials mostly conducted in North America and Western Europe were selected. As seen in Table 3, the immunogenicity of PCV-15 and PCV-13 for shared serotypes were almost similar with a mean OPA GMT ratio ranging from 0.95 to 1.11 according to studies [Reference Platt50, Reference Simon51]. In another trial, the mean PCV-20:PCV-13 OPA GMT ratio for shared serotypes was 0.84, suggesting a somewhat lower immunogenicity for the vaccine with an extended valency [Reference Essink52]. In pneumococcal vaccine-experienced adults, two trials compared OPA responses to PCV-21 or PCV-15, and the mean PCV21:PCV15 OPA GMT ratio for the common serotypes was, respectively, 0.89 and 1.08 [Reference Platt53, Reference Scott54]. In another trial in pneumococcal vaccine naïve adults, the PCV-21: PCV-20 ratio was 1.09 for the 10 shared serotypes [Reference Platt53]. As for children, we do not have a direct comparison between PCV-15 and PCV-20 in adults. In indirect comparisons using PCV13 OPA GMTs as a common denominator, the PCV-20:PCV15 GMT OPA ratio was 0.94 comparing Essink’s and Platt’s results and 0.84 comparing Essink’s and Simon’s results [Reference Platt50–Reference Essink52]. All these results suggest that, in adults, PCV-13 is more immunogenic than PCV20 and that PCV15 and PCV21 are close to PCV13.

It appears that an increase in the number of polysaccharide antigens in pneumococcal conjugate vaccines is associated with a decrease in antibody responses. Our analysis was based on OPA measurements but the same conclusion was drawn in a recent review paper focusing on IgG measurements [Reference Feemster55]. Several biological mechanisms have been proposed to explain this phenomenon, a very plausible one being the “carrier-induced epitotic suppression” resulting from a competition between anti-polysaccharide and anti-peptide-carrier responses [Reference Dagan56, Reference Borrow57]. Results presented in Table 3 suggest that, in children, PCV-7 is slightly more immunogenic than PCV-13, which is slightly more immunogenic than PCV-15 and PCV-21, with the latter two vaccines being equivalent in a 2 + 1 schedule. In adults, PCV-13 and PCV-15 seem to be equivalent, PCV-20 seems to be slightly less immunogenic than the former two vaccines, and PCV-21 seems to lie between PCV-15 and PCV-21. The clinical significance of these differences is not known in terms of direct protection and its duration, as well as the induction of a herd effect. Nevertheless, the induction of higher functional antibody levels is an asset for any vaccine.

Synthesis: Strengths and limitations of vaccines

Ideally, a pneumococcal conjugate vaccine should contain a broad range of serotypes covering a high proportion of IPD cases occurring in the entire population, especially serotypes with high invasiveness, high virulence, and high antimicrobial resistance. In addition, an ideal vaccine should induce a strong immune response towards all pneumococcal polysaccharides in its composition leading to a high level of direct protection against IPD and also against nasopharyngeal infection by invasive serotypes. New-generation PCVs do not meet all these criteria.

For children, the choice in Canada is between replacing PCV-13 with either PCV-15 or PCV-20. PCV-20 seems to be by far the strongest candidate: it contains five serotypes not in PCV-15 currently causing 15% of IPD cases in the group less than 5 years of age. Some of these serotypes are highly invasive (8 and 12F particularly) or of high virulence (11A). PCV-20 does not have a significant advantage over PCV-15 in covering Sp strains resistant to antimicrobials in Canada. In terms of immunogenicity and their capacity to induce functional OPA antibodies, the two new-generation pediatric PCVs have a similar profile. Both PCV-15 and PCV-20 are slightly less immunogenic than PCV-13 and this may have consequences on the duration of protection and also the maintenance of a herd effect against antibody-resistant serotypes such as 19A and 19F, no herd effect being expected against ST 3 [Reference LeBlanc58]. A major advantage of PCV-20 over PCV-15 for children is the low probability of a negative impact on the IPD risk in adults. Results of a dynamic model calibrated on the epidemiological situation in the United Kingdom indicate that PCV-15 use scenarios would be associated with an overall increase in the incidence of IPD, mainly in adults, (estimated at approximately 7% by the end of a 25-year period in the base-case scenario) whereas PCV-20 scenarios would translate into a sustained reduction in the IPD rate (about 12% in the base-case) [Reference Choi38]. These divergent trends are mainly explained by the relative invasiveness of replacing non-vaccine serotypes compared with vaccine serotypes. This signal of a negative effect cannot be ignored.

For elderly adults, PCV-21 is the strongest candidate, especially if PCV-15 or PCV-20 is used in children, as this would likely lead to an increase in the risk of IPD and also non-invasive pneumococcal pneumonia caused by PCV-21-specific serotypes. Another advantage of PCV-21 over PCV-20 for elderly adults is its capacity to prevent IPD caused by virulent serotypes particularly ST 31 and ST 35B.

For adults at very high risk of IPD (i.e. immunocompromised individuals), a sequential PCV-20 + PCV-21 vaccination would be the most effective schedule. Another advantage of a dual vaccination is the boosting of the response to ST-3 and ST19-A, which have elevated correlates of protection.

Finally, a pneumococcal programme in Canada using only two products, PCV-20 and PCV-21, would be easy to implement and manage.

In Europe, PCV-20 was not authorized in a 2 + 1 schedule for children by the European Medicines Agency based on a lower immune response compared to PCV-13 as measured by IgG concentrations whereas responses were more similar when given as a 3 + 1 regimen [59]. In contrast, PCV-15 was authorized by the European Medicine Agency according to 3 + 1 and 2 + 1 schedules for children [60]. However, when OPA titres are considered, responses to PCV-15 and PCV-20 are very similar both in a 3 + 1 and 2 + 1 schedule although lower than PCV-13 responses by about 25% [Reference De Wals49].