Introduction
On farms with intensive livestock production, land application of manure remains a dominant source of nitrogen (N) for crop production. In Denmark, the manure application rate to a given crop is usually defined by its content of ammoniacal-N, assuming that ammonium in manure is as effective as N applied in mineral fertilizers. In other parts of the world, a fraction of the organic N in manure is assumed to be available for the first crop after mineralization (Karimi et al., Reference Karimi, Akinremi and Flaten2018). However, animal manures vary in chemical composition and other characteristics that may affect their N fertilizer value (Webb et al., Reference Webb, Sørensen, Velthof, Amon, Pinto, Rodhe, Salomon, Hutchings, Burczyk, Reid and Sparks2013). Besides ammonium, animal manures contain organically bound N, the amount of which depends on animal species, feeding, housing system (including beddings) and storage conditions before land application (Sørensen et al., Reference Sørensen, Bechini, Jensen and Köpke2019). Upon mineralization, the organic N in manure may contribute mineral N for subsequent crop uptake but also for potential N loss via nitrate leaching or denitrification. Loss of ammonia by volatilization remains critical to the fertilizer value of manure N for the first crop. However, direct injection of liquid manure or immediate incorporation of solid manure can reduce ammonia volatilization to very low levels (Sommer and Hutchings, Reference Sommer and Hutchings2001). To maximize the N use efficiency (NUE) and minimize negative environmental impacts, the effect derived from mineralization of manure N in the years that follow application has to be accounted for.
In the year of amendment, immobilization of ammoniacal-N and mineralization of labile organic N occur simultaneously and affect the N fertilizer value of the manure. The balance between these processes, termed net N mineralization, often relates to quality parameters such as the C/N ratio (Chadwick et al., Reference Chadwick, John, Pain, Chambers and Williams2000; Peters and Jensen, Reference Peters and Jensen2011), which in turn links to the organic matter composition of the manure. In the years following a single manure application, the residual N effect is typically small and therefore difficult to quantify. For land exposed to frequent annual manure applications, however, the cumulated residual N effects may become significant and should be quantified to allow for adjustment of fresh N inputs. Residual effects of manure application vary as illustrated by Chalk et al. (Reference Chalk, Inácio and Chen2020) in a review of studies based on 15N-labelled manures. Of the total 15N applied, crop recovery (also termed NUE) from various pig, cattle and sheep manures ranged from 1.8 to 15% in the first residual year; in the second residual year the recovery ranged from 1 to 3.7%.
Some studies on the value of manure N in crop production have relied on determination of NUE. To be more readily applicable in practical farming, the NUE of animal manure can be converted into the mineral N fertilizer replacement value (NFRV). The NFRV represents the amount of mineral N fertilizer that can be substituted by a given quantity of N in animal manure (Webb et al., Reference Webb, Sørensen, Velthof, Amon, Pinto, Rodhe, Salomon, Hutchings, Burczyk, Reid and Sparks2013). Compared with NUE, however, the determination of NFRV is experimentally more demanding as one or more reference treatments with mineral N fertilizer using the same crop under comparable soil and atmospheric conditions are required.
Results reported by Jensen et al. (Reference Jensen, Sørensen, Thomsen, Jensen and Christensen1999) indicated that the residual effects of manures are mainly determined by the input of total N with manures, whereas other studies have indicated that other factors influence the residual effect (e.g. Sørensen et al., Reference Sørensen, Thomsen and Schröder2017 and Chalk et al., Reference Chalk, Inácio and Chen2020). The objectives of the present study were: (a) to examine the effect of solid material content and thus C/N ratio, in cattle and pig slurries on manure N fertilizer value in the application year and the following 2 years, and (b) to quantify the mineralization rate of residual organic manure N in soil following application of different manure qualities in relation to climatic conditions (growing degree days (GDD)).
Material and methods
Site and soil
Field micro-plots were established in 2017 at Research Centre Foulum (56°30′N, 09°35′E, elevation 41 m above sea level), Aarhus University, Denmark, by inserting polyvinyl chloride (PVC) cylinders (diameter, 30 cm; length 30 cm) into the soil to a depth of 25 cm. The soil is a sandy loam with 80 g clay/kg (<2 μm), 125 g silt/kg (2–20 μm), 760 g sand/kg (20–2000 μm), 35 g organic matter/kg and pH (H2O) 6.8 (Sørensen et al., Reference Sørensen, Weisbjerg and Lund2003). Altogether, 60 cylinders were installed in two rows with 0.5 m between rows and between cylinders within a row. The field received manures regularly until 2015.
Animal manures
Two animal manures, a dairy cattle slurry stored outdoors in a covered slurry tank for several months before sampling, and a pig slurry retrieved directly from underneath a stable facility with 7–30 kg piglets, were available for this study. The manures were separated into two fractions by centrifugation at an acceleration of 4600 g for 4 and 2 min for pig and cattle slurry, respectively. The supernatant (liquid fraction, L) was isolated from the solid fraction (S) by decantation. Table 1 shows the chemical composition of the pig and cattle slurry fractions and the proportion between solid and liquid fractions after centrifugation.
Chemical composition of the solid and liquid fractions of cattle and pig slurry
Numbers in parentheses are contents of ammonium N in slurry fractions measured after 2 months of storage at 2°C. Proportion indicates the % of the unseparated slurry isolated as liquid and solid fraction after separation by centrifugation and decantation.
Subsequently, liquid and solid fractions were recombined to create five cattle and five pig slurry mixtures, targeting mixtures for which 0, 25, 50, 75 and 100% of the NH4+ originated from the solid fraction. Table 2 shows the proportion of solid and liquid fraction in each mixture and the resulting properties of the slurry mixtures. With this approach, the treatments also differed in amounts of slurry dry matter, fibrous organic matter and organic N.
Properties of the cattle and pig liquid−solid slurry mixtures and field amendment rates
L = liquid fraction and S = solid fraction.
Experimental setup
Besides the ten slurry treatments, the experiment included five reference treatments dressed with increasing rates of mineral N fertilizer. The treatments were organized in a randomized design with four blocks. The experiment lasted 3 years (2017, 2018 and 2019) with slurry mixtures applied in spring 2017 only. The application rate corresponded to 150 kg NH4+-N/ha for cattle slurries and 142 kg NH4+-N/ha for pig slurries (Table 2), based on preliminary analyses.
The slurry treatments received no mineral N fertilizer throughout the experiment. The five reference treatments with mineral N fertilizer received 0, 100, 150, 200 and 250 kg N/ha in the spring 2017 as ammonium-nitrate solution. These treatments received another 0, 50, 75, 100 and 150 kg N/ha in the spring 2018 and 2019 and the same rates after each of the first three cuts in 2018 and two cuts in 2019. Total N fertilizer applications were thus 0, 200, 300, 400 and 600 kg N/ha in 2018 and 0, 150, 225, 300 and 450 kg N/ha in 2019.
The slurry mixtures were applied on 2 May 2017 by simulating injection to 10 cm depth. The 0–5 cm of soil was removed from the cylinder, the slurry placed in a 5 cm deep furrow and the slurry band then covered with soil immediately after. A similar procedure was used for mineral N fertilizer treatments. The following day, spring barley (Hordeum vulgare L., cv. KWS Irina) was planted at 3 cm soil depth with a row spacing of 12 cm (368 seeds/m2) and undersown with perennial ryegrass (Lolium perenne L., cv. Calvano). The grass seeds were surface-applied (8 kg seeds/ha) and lightly incorporated into the soil. Subsequently, a nutrient solution containing P, K, S, Ca and Mg at rates corresponding to 50, 198, 17, 100 and 50 kg/ha was added. Another nutrient solution including micronutrients (Mn, Zn, Mo, Cu, B and Co) was added, and similar nutrient solutions were added again in spring 2018 and 2019. The area surrounding the plots received standard rates of mineral fertilizers and was seeded to spring barley.
The spring barley was harvested as a whole-crop on 18–19 July 2017 and one cut of grass taken on 23 October 2017. In 2018, the grass crop was harvested four times (31 May, 11 July, 6 September and 24 October). In 2019, three cuts were taken (12 June, 7 July and 9 September), leaving a stubble of approximately 5 cm after each cut.
Climate
The mean air temperature during the growing season (April–October) was 12.3°C in 2017, 14.2°C in 2018 and 12.5°C in 2019 (1961–1990 mean; 11.6°C). Precipitation during this period was 564, 340 and 562 mm in 2017, 2018 and 2019, respectively (1961–1990 mean; 402 mm). In the warm and dry spring and summer of 2018, irrigation water was added to the cylinders three times (140 mm in total). Figure 1 shows monthly precipitation and mean temperatures.
Monthly precipitation and irrigation in mm (grey bars) from April 2017 to October 2019 and monthly average precipitation (1961–1990, black lines). Actual and mean monthly temperatures in 0C (full line with full circles and dotted line with open circles, respectively) for the same period.
Analytical methods
To establish the application rates of slurries, the solid and liquid fractions were subject to preliminary analyses for total N and ammoniacal-N after separation but before mixing. Total N was measured by Kjeldahl digestion (Tecator Kjeltec Auto 1030) and ammoniacal-N was determined by an automated distillation-titration method (Sommer et al., Reference Sommer, Kjellerup and Kristjansen1992). Following 2 months of storage at 2°C, the liquid and solid slurry fractions were re-analysed for total N and ammoniacal-N, and concentrations of P, K and S determined by ICP-OES (Inductively Coupled Plasma – Optical Emission Spectrometry). Total C content was determined by high-temperature dry combustion.
The barley and grass biomasses were chopped and subsamples dried at 60°C for 24 h to determine dry matter content. Total N of plant material was measured by high-temperature dry combustion.
Calculations
Nitrogen uptake efficiency of mineral N fertilizer (NUEMN) was determined by plotting N uptake in the grass (plus barley in 2017) against total N amendment.Nitrogen uptake efficiency of slurry mixtures (NUEslurry) was calculated as:
where NuptSlurry is the N uptake in the slurry treatment and Nupt0N is the N uptake in the unfertilized reference treatment. Napplicationslurry is the N applied in the slurry treatments in 2017.
The NFRV was calculated as:
For calculating NFRV in 2018 and 2019, NUEslurry was calculated based on total N application in 2017, while NUEMN was calculated based on the mineral N amendment in 2018 and 2019, respectively.
Assuming that N mineralized from slurry and N added in mineral fertilizer is taken up by plants with the same efficiency, NFRV provides an estimate of net N mineralized from the slurry in 2018 and 2019 (Sørensen et al., Reference Sørensen, Thomsen and Schröder2017).
Mineralization of slurry N was related to residual manure N left in soil after harvest of the first barley crop estimated as:
By this calculation it is assumed that the proportion of unknown N losses (gaseous or leaching losses) were similar from applied mineral N and N in slurry mixtures.
Based on temperature recordings at Research Centre Foulum, thermal time after application of animal slurries was expressed as GDD based on air temperatures above 5°C where each day is multiplied with temperature minus 5°C (Bhogal et al., Reference Bhogal, Williams, Nicholson, Chadwick, Chambers and Chambers2016).
Statistical analysis
Statistical analysis was carried out in R (R Core Team, 2018). Post hoc analysis was carried out with the emmeans package. A significance level of P < 0.05 was used throughout. Based on visual inspection of quantile-quantile-plots (QQ-plots), data were found to have a normal distribution and the variance homogeneity was evaluated by inspection of residuals.
Mineral N reference curves were established using linear regression with N uptake as response variable and N application as explanatory variable. A linear mixed model was defined for each of the four response variables: dry matter, N uptake, NUE and NFRV for slurry treatments with year, slurry mixture (proportion of NH4-N from solid fraction) and manure type as fixed effects (including interactions) and plot and block as random effects (Bates et al., Reference Bates, Mächler, Bolker and Walker2015). Pairwise comparisons of means within the year and manure type were conducted using the Tukey's honestly significant difference (HSD) test.
Linear models relating NUE to a ratio of ammonium from solid fraction, a ratio of organic N from solid fraction, C/N ratio, C/Organic N ratio and ammonium to total N ratio were compared using the Akaike's information criterion (AIC) within each year. The AIC is a tool used to compare models and describes the relative amount of lost information. Therefore, the lower the AIC the better the model. Nitrogen mineralization as a function of GDD was analysed using linear regression with GDD, slurry mixture (proportion of NH4-N from solid fraction) and manure type as explanatory variables.
Results
Mineral N fertilizer response
Figure 2 shows total dry matter yield and N uptake in the reference treatments with mineral N fertilizers. For all 3 years, dry matter yield and N uptake increased linearly with N application rate. NUEMN was 79% in 2017, declining to 55% in 2018 and 37% in 2019.
Dry matter yield (a) and total N uptake (b) in mineral N reference treatments shown for 2017 (barley + one grass cut), 2018 (four grass cuts) and 2019 (three grass cuts) related to a total annual mineral N application rate. Error bars indicate standard error (n = 4).
For the mineral N fertilizer treatments, NUEMN was calculated for each harvest (Table 3). NUEMN was high for the spring barley grown in 2017 (80%). The grass cut taken in autumn 2017 was not fertilized with N and NUEMN could not be established. In 2018 and 2019, the first grass cut showed the greatest NUEMN and plant N uptake at 0N fertilizer. Plant N uptake for 0N treatments and NUEMN were small for the last two cuts in 2019. Without N fertilization, cumulated plant N uptake was 77 kg N/ha in 2017, 69 kg N/ha in 2018 and 33 kg N/ha in 2019.
Nitrogen uptake efficiency of mineral N fertilizer (NUEMN) for each harvest based on a linear regression between N application rate and crop N uptake
The first grass cut after barley harvest in 2017 was not fertilized with N. Crop N uptake at unfertilized reference plots (0N) is also shown.
Fertilizer value of slurries in the year of application (2017)
Dry matter yields of the barley plus the grass cut in 2017 (Fig. 3(a)) were similar for all cattle slurry mixtures, although the L100 : S0 and L0 : S100 slurries tended to produce slightly smaller yields. Yields were higher for pig than for cattle slurries and appeared to increase with increasing proportions of solid fraction from pigs. An increase in N uptake with increasing proportion of solid fraction was seen for pig but not for cattle slurries (Fig. 3(b)), however, total N application also increased with increasing proportion of solid fraction. The NUEslurry (Fig. 3(c)) related to the total N amendment (Table 2) decreased when the proportion of solid fraction increased. This was true for the pig as well as cattle slurry when excluding the L100 : S0 cattle slurry. For both cattle and pig slurry, the NUEslurry of L75 : S25 was significantly higher than that of L25 : S75 and L0 : S100.
Dry matter yield of barley plus grass (a), crop N uptake (b) and N uptake efficiency (NUEslurry) for total N applied in pig and cattle slurry mixtures (c) in 2017. Explanation of mixtures is given in Table 2. Bars indicate means and error bars show standard errors, and letters indicate significant differences within each slurry type (cattle and pig) analysed with a Tukey test (n = 4).
The linear relationships between NUEslurry and the proportion of ammonium N or organic N derived from solid fraction, the C/N ratio, the ratio C/organic N, and ammonium to total N ratio were compared using AIC. The ratio of ammonium originating from the solid fraction described the relationship best (Table 4). Generally, NUEslurry of cattle slurry mixtures were poorly predicted by all the tested parameters. For the pig slurry mixtures, NUEslurry was fairly well predicted by C/N and ammonium to total N ratio.
Relationship between nitrogen uptake efficiency in the first year barley plus grass crop from cattle and pig slurry mixtures (Y, NUEslurry) and NH4-N proportion from solid fraction (%), C/N ratio, C/organic N ratio, organic N proportion from solid fraction (%) and NH4-N/total N ratio
In the first barley crop, NFRV related to total N application similarly decreased with increasing proportion of solid fraction (Table 5) with a few exceptions such as L100 : S0 v. L75 : S25 from cattle. The largest difference between cattle and pig slurries was observed for mixtures containing only liquid fractions. With no solid fraction present, NFRV was 58% for cattle and 70% for pig slurry. Overall NFRV of the pig slurries was significantly higher than the cattle slurries.
Nitrogen fertilizer replacement value (NFRV) of slurry mixtures measured over three growing seasons related to total N applied in 2017 and to residual manure N in soil after barley harvest
No significant interactions between mixture and slurry type (P < 0.05).
Explanation of mixtures is given in Table 2. Small letters indicate significant differences within slurry type (cattle and pig) and year, and different capital letters indicate a significant difference between averages of cattle and pig slurries within each year analysed with a Tukey test (n = 4).
Fertilizer value of slurries in the residual years (2018 and 2019)
Figures 4(a) and (b) show plant dry matter yields and N uptake in the two residual years. Yields and N uptake were considerably smaller in 2019 than in 2018 and this was also true for the reference treatments with mineral N fertilizer additions (Fig. 2). In 2018, no significant differences in dry matter yields and N uptake were seen for treatments with cattle slurries regardless of mixing ratio between solid and liquid fractions. In contrast, yields and N uptake were greater for pig slurries with more than 50% of the ammonium originating from solid fraction than for pig slurries with less solid fraction. In the second residual year (2019), yields and N uptake were similar for all treatments. When N uptake in 2019 was related to the total N applied in 2017 to calculate NUE, no significant differences were seen between the slurry mixtures from both cattle and pigs (Fig. 4(c)). The NUEslurry for cattle and pig slurries averaged 4% (3–5%) and 10% (6–16%) in the first residual year (2018). For the second residual year (2019), NUEslurry was reduced to 0–5%, with an average of 1% for cattle slurries and 3% for pig slurries.
Dry matter yield in the grass (a), crop N uptake (b) and NUEslurry (c) after application of different pig and cattle slurry mixtures determined for 2018 and 2019 and based on total slurry N applied in 2017. Explanations of mixtures are given in Table 2. Bars indicate means and error bars show standard error. Letters indicate significant differences within slurry type (cattle and pig) and year, analysed with a Tukey test (n = 4).
No significant linear relationships were found in 2018 and 2019 between NUEslurry and proportion of ammonium derived from the solid fraction, organic N from solid fraction, C/N ratio, the ratios C/organic N, or ammonium to total N ratio (data not shown).
In 2018, the NFRV related to total N application averaged 6.5% (6–8%) for cattle slurries and 18% (11–29%) for pig slurries (Table 5). The NFRV in 2019 ranged from 1 to 9% for cattle (mean 3.8%) and from 2 to 13% for pig slurries (mean 7.5%).
Cumulated nitrogen uptake in slurry treatments
Table 6 shows the cumulated plant N uptake throughout the experimental period. The N uptake did not differ significantly among cattle slurry treatments. Generally, total N uptake was higher for the pig than for cattle slurries. Treatments receiving pig slurries with 50% or more solid fraction showed significantly higher plant N uptake than treatments with a less solid fraction. When the N uptake is related to the total N input (NUEslurry), differences between pig and cattle slurry diminished. Most differences in NUEslurry among different ratios were not significant, although NUEslurry tended to be smallest when slurries only contained the solid fraction. Figure 5 shows that most of the differences in cumulated NUEslurry among cattle slurries were established already in 2017 at barley harvest. For the pig slurry mixtures, the differences in cumulated NUE decreased over time if we disregard the exceptional L50 : S50 treatment.
Cumulated N uptake efficiency (NUEslurry) of total N applied in slurry mixtures in 2017 during three cropping seasons. Error bars show standard errors (n = 4).
Cumulated apparent recovery of slurry N (sum of 2017, 2018 and 2019) expressed as kg N/ha (after subtraction of crop N uptake in 0N plots) and nitrogen uptake efficiency (NUEslurry) based on slurry total N applied in 2017
No significant interactions between mixture and slurry type (P < 0.05).
Explanation of mixtures is given in Table 2. Small letters indicate significant differences within slurry type (cattle and pig), and different capital letters indicate a significant difference between averages of cattle and pig slurries by a Tukey test (n = 4).
Availability of residual nitrogen and growing degree days
The residual manure N left after barley harvest was estimated as total N applied minus NFRV in the barley crop.
The net mineralization of residual manure N during the following 2 years was estimated based on the NFRV calculated for each grass cut, assuming no mineral N left at barley harvest. Thus, we ascribe fertilizer effects seen after barley harvest to mineralization of residual organic N, including re-mineralization of previously immobilized ammoniacal N. Mineral N fertilizer was not applied to the first grass cut in autumn 2017, and we assume a NUE of 49% of mineralized N, based on a previous study on the same soil type and location (Li et al., Reference Li, Sørensen, Li, Petersen and Olesen2015), to calculate net N mineralization in the first autumn.
Figure 6 shows the relationship between GDD and cumulated net N mineralization, calculated as a percentage of residual slurry N estimated to remain in soil after barley harvest in 2017. There was a large variation in net N mineralization of residual N estimated for the period until the second grass cut (one in 2017 and one in 2018) both within and between treatments. This variation is reflected in the cumulated numbers and the error bars in Fig. 6. The average mineralization rate for pig slurries was 0.0203%/GDD in the period 0–2000 GDD. From 2000 GDD and throughout the experiment, a nearly linear relationship was observed between GDD and cumulated net N mineralization. In the following, we assumed a linear relationship as a proxy even though a slight decrease in N mineralization rate appeared after 4000 GDD for some of the slurries. During 2018 and 2019, the average mineralization rate of residual N was 0.0059%/GDD for cattle mixtures and 0.0105%/GDD for pig mixtures. Excluding the deviant L50 : S50 treatment, the mineralization rate of organic N from pig slurries became 0.0088%/GDD. Averaged across 2018 and 2019 and all slurry mixtures, the N mineralization rate after 2000 GDD was 0.0084%/GDD.
Cumulated net mineralization of residual organic manure N left in the soil after harvest of the first barley crop in treatments receiving slurry mixtures in 2017 as a function of growing degree days (GDD) since application with a base temperature of 5°C. Mineralization of N taking place before the first grass cut in 2018 is not included in the regressions. Error bars show standard errors (n = 4).
The period from barley harvest to the first grass cut in autumn 2017 showed a decrease in NFRV corresponding to 3.5% of the residual N in treatments with cattle slurries, indicating a slight net N immobilization due to the cattle slurry in this period (Fig. 6). In 2018, the average net mineralization from cattle slurry mixtures was equivalent to 15% of the residual slurry N and in 2019 the mineralization was equivalent to 7% of residual N. For treatments with pig slurries, the average mineralization in autumn 2017 (Fig. 6) was equivalent to 13% of the residual slurry N. In 2018, the average mineralization from pig slurries was equivalent to 35% of the residual pig slurry N in soil and in 2019 equivalent to 13% of the residual N.
Discussion
Fertilizer value of slurries in the year of application
In the year of application (2017), the NFRV ranged from 46 to 66% for cattle slurries and from 36 to 70% for pig slurries (Table 5). Except for the slurry with no solid fraction, the differences between pig and cattle slurries with similar proportions of liquid and solid fractions were small. Our first-year effect of N added with slurry is in accordance with Sørensen et al. (Reference Sørensen, Bechini, Jensen and Köpke2019) who ascribe a potential NFRV of 40–80% to pig slurry and 35–65% to cattle slurry and report the availability of N to be lower for solid than for liquid fractions.
Sørensen and Thomsen (Reference Sørensen and Thomsen2005) did a similar separation of pig slurry after the slurry had been stored anaerobically for a few weeks. Using the same approach as here, they found NFRVs of 50, 98 and 81% for the solid fraction, the liquid fraction and the unseparated slurry, respectively. Thus, their first-year fertilizer values were somewhat higher than in the present study, especially for the liquid fraction. A reason for this could be that the liquid fraction contained more decomposable carbon in our study as the pig slurry was not stored prior to separation, which would cause extra N immobilization by microbial decomposition and a lower N fertilizer value for the first crop. The pig slurry used by Sørensen and Thomsen (Reference Sørensen and Thomsen2005) was 15N-labelled, and when they calculated the NFRV using the 15N recovery in the crop they found slightly lower fertilizer values of 48, 91 and 75% for the solid fraction, the liquid fraction and the unseparated slurry, respectively. These estimates of NFRV for the solid fraction were lower with our pig solid fraction (36%) and with our pig liquid fraction (70%).
Presumably most of the fibrous solid fraction derived from faeces and in the year of application, Jensen et al. (Reference Jensen, Sørensen, Thomsen, Jensen and Christensen1999) found NFRV values of 15% for 15N-labelled sheep faeces that were mixed with straw and urine and applied to soil planted to spring barley and undersown with grass. Using a similar experimental set-up, but using winter wheat as a test crop, Bosshard et al. (Reference Bosshard, Sørensen, Frossard, Dubois, Mäder, Nanzer and Oberson2009) observed recoveries of 15N in wheat of 10% for faeces and 47% for mineral N fertilizer, providing NFRV of 21% for the faecal N. The higher NFRV of 46% for the cattle solid fraction compared to that of cattle faecal N indicates that a significant part of the solid fraction derived from urine with a much higher N availability.
Sørensen and Thomsen (Reference Sørensen and Thomsen2005) observed that NFRV exceeded the ammonium content of pig slurry, indicating a net mineralization of organic pig slurry N during the year of application. We also saw net N mineralization after barley harvest for pig, but not for cattle slurries. However, this difference may derive from the different pre-experiment storage period of the two slurries. The liquid and solid fractions of the slurries were mixed based on the ammonium and total N contents determined in the preliminary analyses shortly after slurry separation. While the cattle slurry had been stored for several months before used in this experiment, the available pig slurry was retrieved freshly from the piglet stable facility and applied in the field after a few days of storage. To get a measure of easily mineralizable organic N in the slurry the cattle and pig slurry fractions were therefore stored anaerobically for 2 months at 2°C and re-analysed. The subsequent analyses showed an increase in ammonium content of the pig solid and liquid fractions compared to the preliminary analyses, but no significant increase for the cattle fractions (Table 1). After the 2-month storage period, the ammoniacal-N content in the pig slurry solid fraction increased from 2.2 to 3.4 g/kg. Previous studies showed that anaerobic storage of slurry during a few months had insignificant influence on net N release following application to soil (Sørensen, Reference Sørensen1998).
The NFRV was found to be related to the proportion of solid fraction, but the C/N ratio was also ascribed a considerable part of the variation in NFRV (Table 4). Previous studies have found the C/N ratio to be a good indicator of mineralization potentials. Webb et al. (Reference Webb, Sørensen, Velthof, Amon, Pinto, Rodhe, Salomon, Hutchings, Burczyk, Reid and Sparks2013) report a strong negative correlation between NFRV and C/N ratio based on slurries from experiments with pigs and cattle subjected to different diets. For a spring barley crop, NFRV ranged from 90% for slurry with a C/N ratio of 2 to 60% for slurry with a C/N ratio of 13. A clear impact of the C/N ratio was also shown by Reijs et al. (Reference Reijs, Sonneveld, Sørensen, Schils, Groot and Lantinga2007) and Qian and Schoenau (Reference Qian and Schoenau2002) for a range of different animal manures. Differences in C/N ratio could thus be one explanation for differences among slurry mixtures in first year N fertilizer effect. As shown in Table 2, the C/N ratio of cattle as well as pig slurry mixtures increased with increasing proportion of solid fraction.
The fast N mineralization rate in the first autumn from the pig slurry mixtures may have environmental implications. If the soil is kept vegetation-free in autumn, nitrate leaching may increase whereas there may be a fertilizer effect when the soil is covered with a crop with a long growing season. If pig slurry is applied to crops with a short growing season, the establishment of the following cover crop would be recommendable to reduce the risk of nitrate leaching.
Residual fertilizer value of slurries
The N uptake in grass in 2018 due to mineralization of manure N resulted in an average NUEslurry of 4% and 10% of total N applied in 2017 for cattle and pig slurries, respectively. The N effect was smaller in 2019 with corresponding NUEslurry values of 1% and 3% of the total N applied in 2017 (Fig. 4(c)). For 15N-labelled dairy manure applied to maize, Cusick et al. (Reference Cusick, Kelling, Powell and Munoz2006) recovered 4–8% of the 15N in the first residual year and 1–3% in the second residual year. Corresponding recoveries were 3% and 1.4% for sheep faeces and urine using soybeans and maize as test crops, respectively (Bosshard et al., Reference Bosshard, Sørensen, Frossard, Dubois, Mäder, Nanzer and Oberson2009). For sheep manures composed of 15N-labelled faeces, urine and straw components and applied to a spring barley crop followed by grass, Jensen et al. (Reference Jensen, Sørensen, Thomsen, Jensen and Christensen1999) recorded similar N recoveries for all three components (3.7% in the first residual and 1.4% in the second residual year). For physically separated and 15N-labelled pig slurry, Sørensen and Thomsen (Reference Sørensen and Thomsen2005) found recoveries of 3.5% for the dry matter rich fraction and 2.3% for the liquid fraction in a barley crop in the first residual year.
Assuming a 50% recovery of 15N-labelled mineral fertilizers (Chalk et al., Reference Chalk, Inácio and Chen2020), the residual N effects of the 15N-labelled animal manures above convert to NFRV ranging from 6 to 16% in the first residual year and 3−6% in the second residual year. The smallest values were for urine and liquid manure fractions. In accordance with our results, pig slurry tends to provide larger first-year residual effects than manure derived from ruminants. The NFRV for cattle slurry mixtures was on average 7% in 2018 and 4% in 2019 and aligns with Klausner et al. (Reference Klausner, Kanneganti and Bouldin1994) who found NFRV of 9, 3, 3 and 2% for the first, second, third and fourth residual year, respectively, for dairy manure applied to maize. However, we observed considerably higher NFRV for total N added with pig slurries (18% in 2018 and 8% in 2019, Table 5). Sørensen and Amato (Reference Sørensen and Amato2002) similarly found a high NFRV in barley plus a cover crop in the year after application of pig slurry, equivalent to 11% of the total N applied, on the same soil type as the present study. A much lower residual effect, equivalent to 5% of the total N input, was observed on more clayey soil.
According to the empirical N mineralization model developed by Sørensen et al. (Reference Sørensen, Thomsen and Schröder2017) based on measured NFRV, 17 and 8% of organic N input in cattle slurry becomes mineralized in the first and the second residual year, respectively. This corresponds well to our results when NFRV is related to residual organic N for cattle slurries, showing that 15% of the residual organic N was mineralized in 2018 and further 7% in 2019 (Table 5). For pig slurry, the Sørensen et al. (Reference Sørensen, Thomsen and Schröder2017) model estimates 27% and 10% mineralization of the organic N input in the first and second residual year, respectively. Based on NFRV from the present study, mineralization of residual organic N from pig slurry was somewhat higher at 35% in 2018 and 13% in 2019 (Table 5). The NFRV was similar for all liquid−solid mixtures from cattle slurry, while observed differences between liquid−solid mixtures from pig slurry could not be explained by the proportion of solid fraction.
Nitrogen mineralization and growing degree days
We found that net N mineralization rate of residual organic N from cattle slurries was close to zero during 0–2000 GDD. In the subsequent period (2000–5000 GDD), it averaged 0.0059%/GDD. For the pig slurries, the pattern was quite different with an initial fast mineralization rate for pig slurries at 0.0203%/GDD in the period 0–2000 GDD, followed by a lower rate in the subsequent period (2000–5000 GDD) with net N mineralization at 0.0105%/GDD. Bhogal et al. (Reference Bhogal, Williams, Nicholson, Chadwick, Chambers and Chambers2016) estimated net N mineralization from different manure types that were stripped of their ammonium content before being applied to soil. The mineralization of organic manure N was based on N uptake in grass and N leaching in a 5-year study on two sites. They also found that N mineralization could be divided in two distinct phases, but with a quite different pattern compared to our study. In the first phase until 2300 GDD degrees above 5°C, Bhogal et al. (Reference Bhogal, Williams, Nicholson, Chadwick, Chambers and Chambers2016) found that the cumulated mineralization as a percentage of organic N applied was proportional to thermal time with a mineralization rate of 0.0076%/GDD for cattle farmyard manure, cattle slurry and pig farmyard manure, while we found no significant net mineralization from cattle slurry in the period 0–2000 GDD. Bhogal et al. (Reference Bhogal, Williams, Nicholson, Chadwick, Chambers and Chambers2016) found net mineralization of 0.022%/GDD for pig slurry and layer manure in the first phase until 2300 GDD, which was comparable to the 0.0203%/GDD in the period 0–2000 GDD in our study. In the second phase after 2300 GDD (c. 1.5 years), Bhogal et al. (Reference Bhogal, Williams, Nicholson, Chadwick, Chambers and Chambers2016) found no differences between manure types and they reported a low rate of 0.0011%/GDD in one soil, whereas N mineralization could not be detected in the other soil. In contrast, we found significantly higher mineralization in the period 2000–5000 GDD for both manure types.
Conclusions
In the year of application, N associated with the solid fraction in slurry has the lowest plant availability. In the year after application (first year of residual effects), the NFRV related to total N application was higher from pig slurry mixtures than from cattle slurry mixtures.
In the first and second residual year, the net mineralization of residual organic manure N related to GDD was nearly constant in the period 2000–5000 GDD with an average mineralization rate of 0.0059%/GDD for cattle slurry mixtures and 0.0105%/GDD for pig slurry mixtures. The net N mineralization in the period 0–2000 GDD was more variable depending on the liquid-solid fraction ratio and was negligible from cattle slurry mixtures and high from pig slurry mixtures. When estimating the residual value of manures in the year following application, the different N release pattern, with a significantly faster release rate from fresh pig compared to stored cattle slurries, should be accounted for. However, the proportion of solid fraction from a given slurry type has no clear effect on N release related to total N applied and an estimation of residual N effect can be based on total N application for a given slurry type.
Acknowledgment
We wish to thank the technical staff at the Department of Agroecology, Aarhus University, Denmark, especially Margit Paulsen, for advice and assistance both in the field and lab.
Financial support
The research was funded partly by the Department of Agroecology, Aarhus University and by the Graduate School of Science and Technology at Arhus University.
Conflict of interest
The authors declare there are no conflicts of interest.
Ethical standards
Not applicable.
