Hostname: page-component-76d6cb85b7-lcgwf Total loading time: 0 Render date: 2026-07-27T19:34:25.138Z Has data issue: false hasContentIssue false
  • English
  • Français

Staining of nasal mucosa to examine remodelling

Published online by Cambridge University Press:  02 September 2008

S K Ahmed ,
J L Williams ,
A Drake-Lee  and
S Egginton
S K Ahmed*
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Birmingham NHS TrustUK
J L Williams
Affiliation:
Angiogenesis Research Group, Centre for Cardiovascular Sciences, University of Birmingham, UK
A Drake-Lee
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Birmingham NHS TrustUK
S Egginton
Affiliation:
Angiogenesis Research Group, Centre for Cardiovascular Sciences, University of Birmingham, UK
*
Address for correspondence: Mr Shahzada K Ahmed, Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Birmingham NHS Trust, Birmingham B15 2TH, UK. Fax: (+44) 121 449 0055, E-mail: s.ahmed@doctors.org.uk
Get access Rights & Permissions [Opens in a new window]

Abstract

Background and objective:

The process of embedding tissue in paraffin degrades many important molecules involved in respiratory epithelial remodelling. We therefore examined alternative methods.

Methods:

Inferior turbinate and nasal polyp biopsies were either placed in formalin or immediately snap-frozen in the operating theatre. Novel protocols for staining remodelling markers were compared with current methods.

Results:

Our method, using a mixture of three lectins, stained a significantly greater proportion of samples, compared with using Ulex europeaus lectin alone (84 vs 62 per cent; p < 0.005). Comparison of different proliferation markers showed that Ki67 was more suitable than proliferating cell nuclear antigen for frozen sections.

Conclusions:

This study indicates that our robust, repeatable methods for examining whole mounts and for staining capillaries, cell proliferation and nuclei on the same section of nasal mucosa are superior to current methods. The use of fresh tissue that has not been paraffin-embedded would allow a greater suite of epitopes to be examined in the future.

Keywords

RemodellingNasal MucosaPolypTurbinatesHistology

Information

Type
Short Communications
Information
The Journal of Laryngology & Otology , Volume 123 , Issue 2 , February 2009 , pp. 234 - 237
Copyright
Copyright © JLO (1984) Limited 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

1Watelet, JB, Van Zele, T, Gjomarkaj, M, Canonica, GW, Dahlen, SE, Fokkens, W et al. Tissue remodelling in upper airways: where is the link with lower airway remodelling? Allergy 2006;61:1249–58CrossRefGoogle ScholarPubMed
2Mumbuc, S, Karakok, M, Baglam, T, Karatas, E, Durucu, C, Kibar, Y. Immunohistochemical analysis of PCNA, Ki67 and p53 in nasal polyposis and sinonasal inverted papillomas. J Int Med Res 2007;35:237–41CrossRefGoogle ScholarPubMed
3Williams, JL, Cartland, D, Hussain, A, Egginton, S. A differential role for nitric oxide in two forms of physiological angiogenesis in mouse. J Physiol 2006;570:445–54CrossRefGoogle Scholar
4Hamid, SA, Daly, C, Campbell, S. Visualization of live endothelial cells ex vivo and in vitro. Microvasc Res 2003;66:159–63CrossRefGoogle ScholarPubMed
5Capaldi, MJ, Dunn, MJ, Sewry, CA, Dubowitz, V. Lectin binding in human skeletal muscle: a comparison of 15 different lectins. Histochem J 1985;17:81–92CrossRefGoogle ScholarPubMed
6Philpott, CM, Wild, D, Guzail, M, Murty, GE. Variability of vascularity in nasal mucosa as demonstrated by CD34 immunohistochemistry. Clin Otolaryngol 2005;30:373–5CrossRefGoogle ScholarPubMed
7Hassid, S, Salmon, I, Brugmans, M, Dawance, S, Kiss, R, Gabius, HJ et al. Histochemical study of the epithelia of nasal polyps by biotinylated lectins and neoglycoprotein. A comparison with the normal human respiratory epithelium. Eur J Morphol 1997;35:79–86CrossRefGoogle ScholarPubMed
8Huang, H, Jing, D, Li, Z, Zhou, S, Xiao, S, Ma, D et al. Analysis of lectin receptors in normal nasal mucosa, nasal polyp, inverted papilloma and papillary adenocarcinoma. J Laryngol Otol 1993;107:600–2CrossRefGoogle ScholarPubMed
9Hiroshima, K, Iyoda, A, Shibuya, K, Hoshino, H, Haga, Y, Toyozaki, T et al. Evidence of neoangiogenesis and an increase in the number of proliferating cells within the bronchial epithelium of smokers. Cancer 2002;95:1539–45CrossRefGoogle Scholar